Prominent publications by Helen Lu Yin

KOL Index score: 14431

Phosphatidylinositol 4 phosphate (PI4P) is highly enriched in the trans-Golgi network (TGN). Here we establish that PI4P is a key regulator of the recruitment of the GGA clathrin adaptor proteins to the TGN and that PI4P has a novel role in promoting their recognition of the ubiquitin (Ub) sorting signal. Knockdown of PI4KIIalpha by RNA interference (RNAi), which depletes the TGN's PI4P, impaired the recruitment of the GGAs to the TGN. GGAs bind PI4P primarily through their GAT domain, ...

Also Ranks for: Adaptor Proteins |  pi4p tgn |  sorting signal |  transgolgi network |  secondary protein structure
KOL Index score: 14089

Gelsolin is a calcium binding protein that shortens actin filaments. This effect occurs in the presence but not in the absence of micromolar calcium ion concentrations and is partially reversed following removal of calcium ions. Once two actin molecules have bound to gelsolin in solutions containing Ca2+, one of the actins remains bound following chelation of calcium, so that the reversal of gelsolin's effect cannot be accounted for simply by its dissociation from the ends of the ...

Also Ranks for: Actin Gelsolin |  filament severing |  binding proteins |  calcium ions |  nntetraacetic acid
KOL Index score: 13228

BACKGROUND: Phosphatidylinositol 4,5-bisphosphate (PIP(2)) has been implicated in the regulation of the actin cytoskeleton and vesicle trafficking. It stimulates de novo actin polymerization by activating the pathway involving the Wiskott-Aldrich syndrome protein (WASP) and the actin-related protein complex Arp2/3. Other studies show that actin polymerizes from cholesterol-sphingolipid-rich membrane microdomains called 'rafts', in a manner dependent on tyrosine phosphorylation. Although ...

Also Ranks for: Tyrosine Phosphorylation |  actin protein |  vesicle trafficking |  alcohol acceptor |  arp2 3
KOL Index score: 12934

BACKGROUND: Previously, proteomic methods were applied to characterise differentially expressed proteins in microdissected pancreatic ductal adenocarcinoma cells.

AIMS: To report that CapG and a related protein, gelsolin, which have established roles in cell motility, are overexpressed in metastatic pancreatic cancer; and to describe their pattern of expression in pancreatic cancer tissue and their effect on cell motility in pancreatic cancer cell lines.

METHODS: CapG was identified by ...

Also Ranks for: Cell Motility |  pancreatic cancer |  gelsolin expression |  immunohistochemical analysis |  nuclear proteins
KOL Index score: 12676

Gelsolin participates in the reorganization of the actin cytoskeleton that is required during such phenomena as cell movement, cytokinesis, and apoptosis. It consists of six structurally similar domains, G1-G6, which are arranged at resting intracellular levels of calcium ion so as to obscure the three actin-binding surfaces. Elevation of Ca(2+) concentrations releases latches within the constrained structure and produces large shifts in the relative positioning of the domains, ...

Also Ranks for: Calcium Activation |  chemical models |  gelsolin actin |  protein binding |  cell movement
KOL Index score: 12420

Gelsolin complexes with calcium (gelsolin-Ca2+) binds to the ends of actin filaments to which monomers add preferentially during elongation. It forms a stable complex with actin in a low ionic strength solution which does not normally favor the polymerization of actin. Gelsolin-Ca2+ increases the rate of nucleation of actin which precedes polymerization, but decreases the rate of elongation of the filaments. The final average length of filaments formed in the presence of gelsolin-Ca2+ is ...

Also Ranks for: Gelsolin Actin |  polymerization presence |  rate elongation |  calcium binding |  filament length
KOL Index score: 11952

To gain insight into the physiological function of phosphoinositide 3-kinase (PI 3-kinase) lipid products, this study examines the interactions of the D-3 phosphoinositides with profilin and the consequent effects on actin dynamics and phosphoinositide turnover. Profilin, a ubiquitous actin-regulating protein, plays a putative role in regulating actin assembly and PLC-gamma 1 signaling in light of its unique interactions with actin and phosphatidylinositol 4,5-bisphosphate ...

Also Ranks for: Lipid Products |  phosphoinositide 3 |  alcohol acceptor |  actin assembly |  fluorescence profilin
KOL Index score: 11345

gCap39 is an actin filament end-capping protein which has a threefold repeated domain structure similar to the N-terminal half of gelsolin. However, unlike gelsolin, gCap39 does not sever actin filaments and dissociates completely from filament ends after calcium removal. We have capitalized on these differences to explore the structural basis for actin filament capping, severing, and their regulation. Using truncated gCap39, generated by limited proteolysis or deletion mutagenesis, we ...

Also Ranks for: Actin Filament |  gelsolin gcap39 |  binding proteins |  structural basis |  severing capping
KOL Index score: 11246

Flightless-I (fliI) is a novel member of the gelsolin family that is important for actin organization during Drosophila embryogenesis and myogenesis. Drosophila fliI and the human homolog FLI both contain the classic gelsolin 6-fold segmental repeats and an amino-terminal extension of 16 tandem leucine-rich repeats (LRR). LRR repeats form amphipathic beta-alpha structural units that mediate protein-protein interactions. Although there are close to 100 known LRR domain-containing ...

Also Ranks for: Fli Lrr |  binding proteins |  gelsolinlike domain |  gelsolin actin |  rich repeat
KOL Index score: 11228

The beta-thymosins are a family of related peptides. Recently, thymosin beta 4 was identified as a significant actin monomer sequestering protein in cells. To determine if other beta-thymosins also bind actin, and how they may participate in the regulation of actin polymerization, we expressed thymosin beta 4 and its major homolog, thymosin beta 10, in bacteria and characterized their interactions with actin. Equilibrium sedimentation studies showed that thymosin beta 4 behaved as a ...

Also Ranks for: Thymosin Beta |  actin monomer |  proteins molecular |  sequestering protein |  animals base
KOL Index score: 11187

Gelsolin is a Ca2+- and polyphosphoinositide-modulated actin-binding protein which severs actin filaments, nucleates actin assembly, and caps the "barbed" end of actin filaments. Proteolytic cleavage analysis of human plasma gelsolin has shown that the NH2-terminal half of the molecule severs actin filaments almost as effectively as native gelsolin in a Ca2+-insensitive but polyphosphoinositide-inhibited manner. Further proteolysis of the NH2-terminal half generates two unique fragments ...

Also Ranks for: Actin Filaments |  protein binding |  native gelsolin |  phosphatidylinositol 45 |  peptide fragments
KOL Index score: 11081

Phosphoinositides such as phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate promote cell survival and protect against apoptosis by activating Akt/PKB, which phosphorylates components of the apoptotic machinery. We now report that another phosphoinositide, phosphatidylinositol 4,5-bisphosphate (PIP2) is a direct inhibitor of initiator caspases 8 and 9, and their common effector caspase 3. PIP2 inhibited procaspase 9 processing in cell extracts and in a ...

Also Ranks for: Phosphatidylinositol Phosphate |  alcohol acceptor |  apoptosis caspase |  pip5kialpha pip2 |  akt pkb
KOL Index score: 10946

The Ca2+-activated actin-binding protein gelsolin regulates actin filament length by severing preformed filaments and by binding actin monomers, stabilizing nuclei for their assembly into filaments. Gelsolin binds to phosphatidylinositol 4,5-bisphosphate (PIP2), with consequent inhibition of its filament severing activity and dissociation of EGTA-resistant complexes made with rabbit macrophage or human plasma gelsolin and rabbit muscle actin. This study provides evidence for an ...

Also Ranks for: Actin Filaments |  pip pip2 |  binding proteins |  endogenous gelsolin |  physical state
KOL Index score: 10762

Phosphatidylinositol 4 phosphate [PI(4)P] is essential for secretion in yeast, but its role in mammalian cells is unclear. Current paradigms propose that PI(4)P acts primarily as a precursor to phosphatidylinositol 4,5 bisphosphate (PIP2), an important plasma membrane regulator. We found that PI(4)P is enriched in the mammalian Golgi, and used RNA interference (RNAi) of PI4KIIalpha, a Golgi resident phosphatidylinositol 4 kinase, to determine whether PI(4)P directly regulates the Golgi. ...

Also Ranks for: Golgi Phosphatidylinositol |  clathrin adaptor |  alcohol acceptor |  4 phosphate |  rna interference
KOL Index score: 10389

The Atg8 autophagy proteins are essential for autophagosome biogenesis and maturation. The γ-aminobutyric acid receptor-associated protein (GABARAP) Atg8 family is much less understood than the LC3 Atg8 family, and the relationship between the GABARAPs' previously identified roles as modulators of transmembrane protein trafficking and autophagy is not known. Here we report that GABARAPs recruit palmitoylated PI4KIIα, a lipid kinase that generates phosphatidylinositol 4-phosphate (PI4P) ...

Also Ranks for: Lysosome Fusion |  confocal microscopy |  autophagy proteins |  autophagosome biogenesis |  lipid kinase

Key People For Actin Filaments

Top KOLs in the world
Thomas **** *******
actin filaments fission yeast arp2 3 complex
John * ******
capping protein actin assembly saccharomyces cerevisiae
Marie‐France *******
actin filaments atp hydrolysis barbed ends
James * *******
dictyostelium myosin actin filaments molecular motors
Mark * ********
brush border actin filaments unconventional myosins
Thomas ***** *******
actin filaments plasma gelsolin phagocytic vesicles

Helen Lu Yin:Expert Impact

Concepts for whichHelen Lu Yinhas direct influence:Actin filaments,  Plasma gelsolin,  Actin cytoskeleton,  Ameboid leukocytes,  Alcohol acceptor,  Gelsolin actin,  Gelsolin ca2,  Collagen phagocytosis.

Helen Lu Yin:KOL impact

Concepts related to the work of other authors for whichfor which Helen Lu Yin has influence:Actin filaments,  Plasma membrane,  Binding protein,  Cell migration,  Signal transduction,  Alcohol acceptor,  Thymosin β4.



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c Department of Physiology ; University of Texas Southwestern Medical Center ; Dallas , TX USA. | Department of Physiology, | Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX; | Departments of Physiology, Universi