Prominent publications by Lennart Philipson

KOL Index score: 15236

The Coxsackievirus and adenovirus receptor (CAR) functions as a virus receptor, but its primary biological function is unknown. A yeast two-hybrid screen was used to identify Ligand-of-Numb protein-X (LNX) as a binding partner to the intracellular tail of CAR. LNX harbors several protein-protein interacting domains, including four PDZ domains, and was previously shown to bind to and regulate the expression level of the cell-fate determinant Numb. CAR was able to bind LNX both in vivo and ...

Known for Virus Receptor |  Numb Protein |  Pdz Domain |  Car Binding |  Mammalian Cells
KOL Index score: 12874

The coxsackie- and adenovirus receptor (CAR) is a transmembrane protein belonging to the immunoglobulin superfamily. The function of CAR as a virus receptor has been extensively analyzed, while its physiological role and expression pattern in adult tissues have remained less clear. CAR associates with epithelial tight junctions in vitro and mediates cell-cell adhesion. Using a set of affinity-purified antibodies, we show that CAR is predominantly expressed in epithelial cells lining the ...

Known for Tight Junctions |  Virus Receptor |  Epithelial Cells |  Tissue Homeostasis |  Transgenic Microscopy
KOL Index score: 11566

The coxsackievirus and adenovirus receptor (CAR) is a transmembrane protein important for viral binding to target cells. Using RT-PCR, Western analysis, GST pull-down assay and indirect immunofluorescence, it was shown that CAR is expressed in male germ cells from mice, rats, and humans. CAR was detected in round spermatids in the testis as well as in purified, mature spermatozoa. The two membrane-bound isoforms of CAR occupied different subcellular sites in the acrosomal region of the ...

Known for Adenovirus Receptor |  Mouse Testis |  Germ Cells |  Cultured Coxsackie |  Membrane Protein
KOL Index score: 11383

Anti-RNP sera were used to isolate a cDNA clone for the largest polypeptide of the U1 snRNP, a protein of mol. wt 70 kd designated 70K, from a human liver cDNA library constructed in the expression vector pEX1. The cro-beta-galactosidase-70K fusion protein reacted with various anti-RNP patient sera, a rabbit anti-70K antiserum, as well as with a monoclonal antibody specific for this protein. The sequences of four 70K peptides were determined and they match parts of the deduced amino acid ...

Known for 70k Protein |  U1 Rna |  Human Cdna |  Amino Acid |  70 Kd
KOL Index score: 11317

The mechanisms controlling early adenovirus gene expression in vivo have been studied using inhibitors of protein synthesis. When inhibitors were added shortly before or at the onset of infection, viral mRNA from all early regions was transcribed, spliced and accumulated over a 7 hr period. After longer pretreatment, accumulation of several early mRNAs were suppressed. Addition of inhibitors 1 hr after infection enhanced the accumulation of viral mRNA in the cytoplasm. Translation of ...

Known for Viral Mrna |  Early Gene |  Messenger Rna |  Protein Synthesis |  Infection Accumulation
KOL Index score: 11137

The subgroup C of the adenoviruses (Ad) and the group B coxsackieviruses (CVB) are structurally unrelated viruses that are known to compete for an unidentified cell surface receptor. We now describe the isolation of cDNAs from human and mouse that encode the human CVB and Ad2 and 5 receptor (HCAR) and the mouse CVB Ad2 and 5 receptor (MCAR). Both are 46-kDa glycoproteins whose primary amino acid sequences are highly homologous. Structurally, HCAR and MCAR appear to be transmembrane ...

Known for Amino Acid |  Complementary Enterovirus |  Coxsackieviruses Cvb |  5 Receptor |  Virus Sequence Homology
KOL Index score: 10430

The localization of the gene(s) mediating methicillin (mecr) in Staphylococcus aureus was determined by transformation with deoxyribonucleic acid (DNA) from a natural mecr strain (DU 4916) and transformation obtained with DNA from this strain. Streptomycin resistance genes (strr) and novobiocin resistance genes (novr) were used concurrently as representatives for chromosomal genes; penicillinase (PI254) and tetracycline plasmids were used as examples of medium- and small-size ...

Known for Staphylococcus Aureus |  Bacterial Dna |  Chromosomal Locus |  Methicillin Resistance Mecr |  Resistance Genes
KOL Index score: 10423

A 29.5-kb plasmid, pSX267, from Staphylococcus xylosus DSM 20267 was found to code for arsenate, arsenite, and antimony (III) resistance. The isolated plasmid was transformed into S. aureus, where the same resistances were expressed. It was of special interest to see whether pSX267 showed any DNA sequence homology with the well-studied penicillinase plasmid from S. aureus pI258, also conferring arsenate, arsenite, and antimony III resistance. By the use of the Southern blotting ...

Known for Arsenate Resistance |  Plasmid Psx267 |  Staphylococcus Xylosus |  Replication Region |  Aureus Pi258
KOL Index score: 10098

CELL growth and differentiation are usually mutually exclusive1. Transformation of myoblasts by retroviruses containing the myc oncogene inhibits differentiation, preventing cells from withdrawing from the cell cycle4,5. If cell-cycle withdrawal is a prerequisite for myoblast differentiation, it is probably an early event in terminal cell differentiation, but this has not yet been established6. MyoD1 regulates myogenesis7. It is expressed only in skeletal muscle8, but can convert other ...

Known for Cell Proliferation |  Cultured Cloning |  Dna Synthesis |  Myogenic Differentiation |  Expression Myod1
KOL Index score: 9875

With high resolution SDS-polyacrylamide gel electrophoresis (Maizel, 1971) and a new method to extract the basic proteins, it was ascertained that adenovirus type 2 contains at least 10 distinct polypeptides (II, III, IIIa, IV–X) and possibly more. Five proteins (V, VI, VII, VIII, and X) were purified by selective extraction in urea at high ionic strength, and low pH followed by preparative polyacrylamide electrophoresis toward the cathode at pH 3.4. Antisera, produced against proteins ...

Known for Adenovirus Type |  Structural Proteins |  Molecular Weight |  Polypeptides Vii |  Density Gradient Electrophoresis
KOL Index score: 9639

Incomplete virus particles were isolated from cells infected with adenovirus type 3. More than 30% of the particles recovered at 72 hr after infection were found to be incomplete.Polyacrylamide electrophoresis revealed that incomplete particles were deficient in the two major core polypeptides, but contained enhanced levels of a polypeptide present in complete virions in only minute amounts. The amino acid composition of incomplete particles showed reduced levels of both arginine and ...

Known for Adenovirus Type |  Incomplete Particles |  Viral Electrophoresis |  Density Gradient Centrifugation |  Structural Proteins
KOL Index score: 9499

Messenger ribonucleic acid (mRNA) from cells productively infected with adenovirus type 2 was isolated by affinity chromatography on polyuridylic acid [poly (U)] bound to Sepharose. At least 90% of the polyadenylic acid [poly (A)]-containing polysomal mRNA was retained by the poly (U) Sepharose and thus separated from more than 95% of the ribosomal RNA and transfer RNA. In these experiments, 65% of the early (3 to 5 hr postinfection) and 85% of the late (14 to 16 hr postinfection) ...

Known for Adenovirus Messenger |  Productive Infection |  Nucleic Acid |  Viral Mrna |  Poly Sepharose
KOL Index score: 9446

In NIH3T3 fibroblasts, the ubiquitous helix-loop-helix (HLH) protein E2A (E12/E47) and the myogenic HLH proteins MyoD, MRF4 and myogenin are growth-inhibitory, while two ubiquitous Id proteins lacking the basic region are not. The dimerization domain mediates inhibition. However, in addition to the HLH region, E2A contains two inhibitory regions over-lapping with the main transcriptional activation domains. The growth-suppressive activity of the intact E47 as well as MyoD was ...

Known for G1 Progression |  E2a Proteins |  Cell Cycle |  Differentiation Protein |  Hlh Domain


Lennart Philipson: Influence Statistics

Sample of concepts for which Lennart Philipson is among the top experts in the world.
Concept World rank
virus particle2 #1
car binding region #1
purified hexon rabbits #1
assembly ts19 #1
erythrocytes host cells #1
methicillin mecr #1
hjertén rna #1
coxsackie viruses6 #1
2liter cultures #1
factor infeccioso #1
mecr genes #1
agarose bengtsson #1
medium 14cacetate #1
dna murine gas5 #1
throat faeces #1
heattreated host bacteria #1
granulated gels filtration #1
poliovirions borate buffers #1
rna ligase activity #1
bal echo 7 #1
erythrocytes virus particles #1
subcultivation passage #1
essential role car #1
rna endonucleases vairna #1
replicative structure polymerase #1
ligase activity transfer #1
40 bases rna #1
deae chromatography gradients #1
echo virus particles #1
approaches growth regulation #1
maximal level analgesia #1
γg antibodies implications #1
rna early regions #1
receptorrecognising portion #1
37° haemagglutination #1
≈4 strains #1
guanidine competitors #1
photography viral proteins #1
narrow phrange33 incorporation #1
nucleoli 1–2 weeks #1
intact monkey kidney #1
werner variety #1
26k vivo #1
8 virologic evidence #1
easily transducible cells #1
coxsackievirus hela cells #1
car jamc #1
0 subsequent incubation #1
initial sampling rise #1
rna adenoviridae carbon #1

Key People For Adenovirus Type

Top KOLs in the world
Ulf Pettersson
adenovirus type trypanosoma cruzi messenger rna
messenger rna gene expression simian virus
Frank L Graham
gene transfer adenovirus vectors early region
Lennart Philipson
adenovirus type messenger rna staphylococcus aureus
Alex J van der Eb
transformed cells adenovirus type e1a proteins
Maurice Green
messenger rna adenovirus type viral dna

Lennart Philipson:Expert Impact

Concepts for whichLennart Philipsonhas direct influence:Adenovirus type,  Motor blockade,  Messenger rna,  Staphylococcus aureus,  Poliovirus rna,  Gel filtration,  Viral mrna,  Adenovirus receptor.

Lennart Philipson:KOL impact

Concepts related to the work of other authors for whichfor which Lennart Philipson has influence:Staphylococcus aureus,  Adenovirus type,  Gene expression,  Cell cycle,  Messenger rna,  Escherichia coli,  Viral dna.



Is this your profile? manage_accounts Claim your profile content_copy Copy URL code Embed Link to your profile

Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden | Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden | Department of Cell and Molecular Biology, Karolinska Institute, 17177, Stockholm,

FREE Custom List