Prominent publications by John Edwin Mole

KOL Index score: 11143

Total cytochrome P-450 levels rise in diabetic rats. Two specific forms of cytochrome P-450 that are elevated have been isolated from liver microsomes of streptozotocin-induced idabetic male rats. One enzyme, termed RLM6, metabolizes aniline and acetol, but not testosterone, in a reconstituted system with NADPH-cytochrome P-450 reductase. RLM6 is isolated as a high spin cytochrome with a minimum molecular weight of 53,500. It has a unique amino-terminal amino acid sequence lacking ...

Also Ranks for: Diabetic Rats |  cytochrome p450 |  liver microsomes |  1 week |  male rat
KOL Index score: 11073

Trypsin inhibitory activity from the hemolymph of the tobacco hornworm (Manduca sexta) was purified by affinity chromatography on immobilized trypsin and resolved into two fractions with molecular weights of 14,000 (M. sexta hemolymph trypsin inhibitor (HLTI) A) and 8,000 (HLTI B) by molecular sieve chromatography on Sephadex G-75. Electrophoresis of these inhibitors under reducing conditions on polyacrylamide gels gave molecular weight estimates of 8,300 for HLTI A and 9,100 for HLTI B, ...

Also Ranks for: Trypsin Inhibitors |  manduca sexta |  polyacrylamide gels |  affinity chromatography |  tobacco hornworm
KOL Index score: 10696

cDNA encoding the precursor of rat liver medium chain acyl-CoA dehydrogenase (EC was cloned and sequenced. The longest cDNA insert isolated was 1866 bases in length. This cDNA encodes the entire protein of 421-amino acids including a 25-amino acid leader peptide and a 396-amino acid mature polypeptide. The identity of the medium chain acyl-CoA dehydrogenase clone was confirmed by matching the amino acid sequence predicted from the cDNA to the NH2-terminal and nine internal ...

Also Ranks for: Chain Acyl |  molecular cloning |  rat liver |  coa dehydrogenase |  leader peptide
KOL Index score: 9624

A protein preparation that specifically binds insulin-like growth factors (IGFs) I and II was purified from medium conditioned by rat liver BRL-3A cells using molecular sieve chromatography in 1 M acetic acid followed by affinity chromatography on IGF-II-agarose. The affinity-purified IGF-binding protein exhibits a single major band with apparent Mr = 36,300 under reducing conditions on sodium dodecyl sulfate-polyacrylamide gels. The IGF-binding protein is efficiently and specifically ...

Also Ranks for: Binding Protein |  affinity chromatography |  rat liver |  brl3a igf |  growth factor
KOL Index score: 9229

Gelsolin is a Ca2+-sensitive 90-kDa protein which regulates actin filament length. A molecular variant of gelsolin is present in plasma as a 93-kDa protein. Functional studies have shown that gelsolin contains two actin-binding sites which are distinct in that after Ca2+-mediated binding, removal of free Ca2+ releases actin from one site but not from the other. We have partially cleaved human plasma gelsolin with alpha-chymotrypsin and identified two distinct actin-binding domains. ...

Also Ranks for: Gelsolin Actin |  binding domains |  free ca2 |  nh2 terminus |  functional studies
KOL Index score: 8837

Addition of tumor-promoting phorbol diesters to [32P]phosphate-labeled A431 human epidermoid carcinoma cells caused an increase in the phosphorylation state of the transferrin receptor. The A431 cell transferrin receptor was also found to be a substrate for protein kinase C in vitro. Tryptic phosphopeptide mapping of the transferrin receptor resolved the same two phosphopeptides (X and Y) after either protein kinase C phosphorylation in vitro or treatment of labeled A431 cells with ...

Also Ranks for: Transferrin Receptor |  protein kinase |  serine 24 |  phosphorylation site |  cytoplasmic domain
KOL Index score: 8542

A new homodimer form of transforming growth factor-beta (TGF-beta), TGF-beta 2, has been identified in porcine blood platelets. TGF-beta 2 is homologous to ordinary TGF-beta (TGF-beta 1), which is also present in platelets. TGF-beta 1.2, a heterodimer containing one TGF-beta 1 chain and one TGF-beta 2 chain, has also been isolated. TGF-beta 1 and TGF-beta 2 interact differently with a family of receptors in target cells. A 280 kd receptor displays high affinity for both TGF-beta 1 and ...

Also Ranks for: Transforming Growth Factor |  beta tgf |  complex pattern |  receptors cell |  tissue growth
KOL Index score: 8379

A new cytochrome P-450 isozyme (RLM2) has been purified to electrophoretic homogeneity from liver microsomes of the untreated rat. It has an apparent minimum molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 49,000. Absolute spectrum of the oxidized form indicates that this isozyme is essentially all in the low spin state. The maximum of the reduced CO complex is at 449 nm. Amino-terminal partial amino acid sequence and amino acid composition are different ...

Also Ranks for: Untreated Rat |  liver microsomes |  cytochrome p450 |  electrophoretic homogeneity |  major metabolite
KOL Index score: 8129

Immunocytochemical methods have been used to compare the cellular and subcellular distribution of nerve growth factor (NGF) and epidermal growth factor (EGF) in mouse submandibular glands. Rabbit antisera raised against purified proteins were characterized by immunoblot methods and were used to stain sections of salivary glands embedded in plastic. For light microscopy, antibodies were visualized by indirect immunofluorescence. For electron microscopy, thin sections were treated ...

Also Ranks for: Nerve Growth Factor |  mouse submandibular glands |  egf ngf |  electron microscopy |  salivary gland
KOL Index score: 7817

Deficiency of pyruvate dehydrogenase [pyruvate:lipoamide 2-oxidoreductase (decarboxylating and acceptor-acetylating), EC], the first component of the pyruvate dehydrogenase complex, is associated with lactic acidosis and central nervous system dysfunction. Using both specific antibodies to pyruvate dehydrogenase and cDNAs coding for its two alpha and beta subunits, we characterized pyruvate dehydrogenase deficiency in 11 patients. Three different patterns were found on ...

Also Ranks for: Pyruvate Dehydrogenase |  alpha subunit |  lactic acidosis |  complex deficiency |  normal amounts
KOL Index score: 6804

Gelsolin is representative of a class of actin-modulating proteins found in lower eukaryotes to mammals, which sever actin filaments1. Gelsolin found in the cytoplasm of cells is functionally similar to a mammalian plasma protein of similar size, originally called ADF or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25-amino-acid residue extension on plasma gelsolin which appears to account for the difference in relative molecular mass (Mr) between the ...

Also Ranks for: Single Gene |  gelsolin plasma gelsolin |  nucleic acid |  human plasma |  binding proteins
KOL Index score: 5786

Gelsolin is an actin-fragmenting cytoplasmic protein. A functionally similar protein has also been identified in plasma. We have compared the structure of the cytoplasmic and plasma forms of gelsolin and examined their biosynthetic relationships. Plasma gelsolin is larger than cytoplasmic gelsolin (Mr 93,000 versus 90,000, respectively) and is more positively charged. Partial amino acid sequencing analyses show that the two gelsolins share a common 29 amino acid sequence which lies at ...

Also Ranks for: Cytoplasmic Gelsolin |  blood platelets |  nh2 terminus |  plasma protein |  93 kda
KOL Index score: 5680

Rat liver mRNA encoding the cytoplasmic precursor of mitochondrial isovaleryl-CoA dehydrogenase was highly enriched by polysome immunopurification using a polyclonal monospecific antibody. The purified mRNA was used to prepare a plasmid cDNA library which was screened with two oligonucleotide mixtures encoding two peptides in the amino-terminal portion of mature rat isovaleryl-CoA dehydrogenase. Thirty-one overlapping cDNA clones, spanning a region of 2.1 kbp, were isolated and ...

Also Ranks for: Coa Dehydrogenase |  cdna clones |  chromosome 15 |  leader peptide |  chromosomal assignment
KOL Index score: 5221

Amino acid sequences deduced from rat complementary DNA clones encoding the insulin-like growth factor II (IGF-II) receptor closely resemble those of the bovine cation-independent mannose-6-phosphate receptor (Man-6-P receptorCI), suggesting they are identical structures. It is also shown that IGF-II receptors are adsorbed by immobilized pentamannosyl-6-phosphate and are specifically eluted with Man-6-P. Furthermore, Man-6-P specifically increases by about two times the apparent affinity ...

Also Ranks for: Insulin Receptors |  growth factor |  receptor igf |  amino acid sequences |  affinity dna

Key People For Amino Acid

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John Edwin Mole:Expert Impact

Concepts for whichJohn Edwin Molehas direct influence:Amino acid,  Transferrin receptor,  Sequence analysis,  Serine proteases,  Cdna clones,  Human factor,  Pyruvate dehydrogenase,  Cyanogen bromide.

John Edwin Mole:KOL impact

Concepts related to the work of other authors for whichfor which John Edwin Mole has influence:Growth factor,  Tgf beta,  Amino acid,  Plasma gelsolin,  Cytochrome p450,  Binding proteins,  Transferrin receptor.



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Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06030, USA and Univ Massachusetts, Sch Med, Dept Biochem, Worcester, MA 01655, USA | Department of Biochemistry, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, M