• Autophagosome Formation
    • Taki Nishimura
    • Taki Nishimura: Influence Statistics

      Taki Nishimura

      Taki Nishimura

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      PRESTO, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0076, Japan | Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The ...

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      Taki Nishimura:Expert Impact

      Concepts for whichTaki Nishimurahas direct influence:Autophagosome formation,Atg proteins,Lipid droplets,Membrane lipids,Lipid metabolism,Novo mutations,Static encephalopathy,Perinuclear localization.

      Taki Nishimura:KOL impact

      Concepts related to the work of other authors for whichfor which Taki Nishimura has influence:Lipid droplets,Endoplasmic reticulum,Autophagosome biogenesis,Autophagy proteins,Brain iron accumulation,Autophagic flux,Neurodegenerative diseases.

      KOL Resume for Taki Nishimura


      PRESTO, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0076, Japan

      Molecular Cell Biology of Autophagy Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK


      Molecular Cell Biology of Autophagy, The Francis Crick Institute, NW1 1AT, London, UK


      MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK. Electronic address:


      University College London


      Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo, Japan


      Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-8655, Japan.


      Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan


      University of Tokyo Graduate School of Pharmaceutical Sciences Tokyo Japan


      Department of Molecular Immunology, Research Institute for Microbial Diseases, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan;


      Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-Ku, Tokyo 113-0033, Japan


      Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113‐0033, Japan


      Graduate School of Pharmaceutical Sciences, University of Tokyo, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo 113‐0033, Japan

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      Sample of concepts for which Taki Nishimura is among the top experts in the world.
      Concept World rank
      biogenesis atg proteins #2
      cismembrane association #2
      gfp stx17δntd #2
      syntaxin macroautophagy autophagy #2
      dominantnegative stx17 #2
      transport unresolved issues #2
      fulllength gfpstx17 #2
      intimate relationships network #2
      gabarap associate #2
      atg proteins autophagosome #2
      understanding mutual relationships #2
      cytoplasmic materials evidence #2
      lipids autophagosome #2
      atg proteins ability #2
      pisenriched subdomains #2
      subdomains initiation #2
      undegraded autophagosomes expression #2
      characterization nascent autophagosomes #2
      autophagosome simple method #2
      gfpstx17 #2
      autophagosomes atg8 proteins #2
      cis membrane association #2
      lipidated human atg8s #2
      membrane lipids atg #2
      subdomain atg9a #2
      accumulation undegraded autophagosomes #2
      autophagosome membrane lipids #2
      autophagosome membrane expansion #2
      gfpstx17 accumulation #2
      undegraded autophagosomes #2
      atg proteins biogenesis #2
      autophagosomes hela cell #2
      vesicles autophagosome formation #2
      ltps activities #2
      hela optiprep #2
      snares gs28 #2
      large undigested autophagosomes #2
      key findings function #2
      gfp stx17 #2
      pis atg9a #2
      organelles intimate relationships #2
      early autophagy factors #2
      dynamic membrane event #2
      Sign-in to see all concepts, it's free!

      Prominent publications by Taki Nishimura

      KOL-Index: 11380

      Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to better understand the mechanism of autophagosome-lysosome fusion, we searched for STX17-interacting proteins. Immunoprecipitation and mass spectrometry analysis identified ...

      Known for Lysosome Fusion | Syntaxin 17 | Hops Complex | Endocytic Pathway | Vps33a Vps16
      KOL-Index: 8939

      25-hydroxycholesterol (25-HC) is a potent suppressor of cholesterol synthesis gene transcription in cultured cells. A high affinity binding protein for 25-HC, oxysterol-binding protein (OSBP), has been identified from tissue cytosol. OSBP translocates from the cytosol to the Golgi apparatus membranes after addition of 25-HC to cell cultures and is thought to mediate 25-HC action on cholesterol metabolism through association to the Golgi apparatus. However, direct evidence to prove this ...

      Known for Osbp Golgi Apparatus | Cholesterol Biosynthesis | 25 Hc | Small Interfering Receptors | Cultured Cells
      KOL-Index: 8485

      Macroautophagy is an intracellular degradation system by which cytoplasmic materials are enclosed by the autophagosome and delivered to the lysosome. Autophagosome formation is considered to take place on the endoplasmic reticulum and involves functions of autophagy-related (Atg) proteins. Here, we report the identification and characterization of mammalian Atg2 homologues Atg2A and Atg2B. Simultaneous silencing of Atg2A and Atg2B causes a block in autophagic flux and accumulation of ...

      Known for Autophagosome Formation | Lipid Droplets | Autophagic Membrane | Autophagy Proteins | Endoplasmic Reticulum
      KOL-Index: 7565

      The autophagosome, a double-membrane structure mediating degradation of cytoplasmic materials by macroautophagy, is formed in close proximity to the endoplasmic reticulum (ER). However, how the ER membrane is involved in autophagy initiation and to which membrane structures the autophagy-initiation complex is localized have not been fully characterized. Here, we were able to biochemically analyze autophagic intermediate membranes and show that the autophagy-initiation complex containing ...

      Known for Autophagosome Formation | Autophagy Initiation | Endoplasmic Reticulum Membrane | Phosphatidylinositol Synthase | Proteins Mice
      KOL-Index: 6996

      Intra-Golgi retrograde transport is assumed to maintain Golgi function by recycling Golgi-resident proteins to younger cisternae in the progression of entire Golgi stack from cis to trans. GS28 (Golgi SNARE of 28 kDa, also known as GOS28) is a Golgi-localized SNARE protein and has been implicated in intra-Golgi retrograde transport. However, the in vivo functions of GS28, and consequently, the roles of the intra-Golgi retrograde transport in animal development are largely unknown. In ...

      Known for Golgi Snare | Caenorhabditis Elegans | Proteins Cell | 28 Kda | Rna Interference
      KOL-Index: 6805

      Hirotomo Saitsu, Noboru Mizushima, Naomichi Matsumoto and colleagues report the identification of de novo mutations in WDR45 that cause static encephalopathy of childhood with neurodegeneration in adulthood. WDR45 encodes a homolog of the yeast autophagy protein Atg18.

      Known for Novo Mutations | Static Encephalopathy | Autophagy Gene | Wdr45 Encodes | Intellectual Disability
      KOL-Index: 6629

      ATG2 is one of the autophagy-related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N-terminal amino acids 1-198 and the C-terminal amino acids 1830-1938 are required for the localization to isolation membranes and lipid droplets, respectively. The C-terminal region is not required for the ...

      Known for Lipid Droplets | Isolation Membranes | Autophagy Proteins | Autophagosome Formation | Amphipathic Helix
      KOL-Index: 5922

      Oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) have been implicated in the distribution of sterols among intracellular organelles. OSBP regulates the Golgi cholesterol level, but how it relates to Golgi function is elusive. Here we report that OSBP is essential for the localization of intra-Golgi soluble vesicle N-ethylmaleimide-sensitive fusion attachment protein receptors (v-SNAREs). Depletion of OSBP by small interfering RNA causes mislocalization of intra-Golgi ...

      Known for Binding Protein | Golgi Localization | Endoplasmic Reticulum | Cholesterol Depletion | Receptors Steroid
      KOL-Index: 5761

      The cytoplasmic lipid droplet (LD) is one of organelles that has a neutral lipid core with a single phospholipid layer. LDs are believed to be generated between the two leaflets of the endoplasmic reticulum (ER) membrane and to play various roles, such as high effective energy storage. However, it remains largely unknown how LDs are generated and grow in the cytoplasm. We have previously shown that the Atg conjugation system that is essential for autophagosome formation is involved in LD ...

      Known for Ld Formation | Lipid Droplet | Control Cells | Endoplasmic Reticulum | Cellular Homeostasis
      KOL-Index: 4916

      Autophagosome formation is a dynamic process that is strictly controlled by autophagy-related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14- and phosphatidylinositol 3-kinase-independent manner. Atg16L1 deletion mutants, which lack the ...

      Known for Isolation Membrane | Autophagosome Formation | Atg Proteins | Interaction Domains | Protein Transport
      KOL-Index: 4837

      Lysophosphatidylcholine (LPC) and oxysterols which are major components in oxidized low-density lipoprotein have been shown to possess an opposite effect on the expression of sterol regulatory element-binding protein-2 (SREBP-2) target genes in endothelial cells. In this study, we aimed at elucidating the mechanisms of activation of SREBP-2 by LPC and evaluating the effects of LPC and 25-hydroxycholesterol (25-HC) on the release of inflammatory cytokines. Human umbilical vein endothelial ...

      Known for Endothelial Cells | Lysophosphatidylcholine Lpc | Inflammatory Cytokines | Oxidized Lowdensity Lipoprotein | Genes Involved
      KOL-Index: 4429

      Germinal centers (GCs) are specialized microenvironments in secondary lymphoid organs where high-affinity antibody-producing B cells are selected based on B-cell antigen receptor (BCR) signal strength. BCR signaling required for normal GC selection is uncertain. We have found that protein kinase N1 (PKN1, also known as PRK1) negatively regulates Akt kinase downstream of the BCR and that this regulation is necessary for normal GC development. PKN1 interacted with and inhibited Akt1 kinase ...

      Known for Protein Kinase N1 | Germinal Center | Inbred C57bl Mice | Autoantibody Production | Gc Cell
      KOL-Index: 4423

      Lysosomes have an important role in cellular protein and organelle quality control, metabolism, and signaling. On the surface of lysosomes, the PIKfyve/Fab1 complex generates phosphatidylinositol 3,5-bisphosphate, PI-3,5-P2, which is critical for lysosomal membrane homeostasis during acute osmotic stress and for lysosomal signaling. Here, we identify the inverted BAR protein Ivy1 as an inhibitor of the Fab1 complex with a direct influence on PI-3,5-P2 levels and vacuole homeostasis. Ivy1 ...

      Known for Vacuole Membrane | Alcohol Acceptor | Fab1 Complex | Saccharomyces Cerevisiae | Osmotic Stress
      KOL-Index: 4184

      Macroautophagy/autophagy, which is one of the main degradation systems in the cell, is mediated by a specialized organelle, the autophagosome. Purification of autophagosomes before fusion with lysosomes is important for both mechanistic and physiological studies of the autophagosome. Here, we report a simple method to accumulate undigested autophagosomes. Overexpression of the autophagosomal Qa-SNARE STX17 (syntaxin 17) lacking the N-terminal domain (NTD) or N-terminally tagged GFP-STX17 ...

      Known for Syntaxin 17 | Hek293 Cells | Macroautophagy Autophagy | Snare Proteins | Fusion Lysosomes
      KOL-Index: 4026

      The plasma membrane (PM) is composed of a complex lipid mixture that forms heterogeneous membrane environments. Yet, how small-scale lipid organization controls physiological events at the PM remains largely unknown. Here, we show that ORP-related Osh lipid exchange proteins are critical for the synthesis of phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2], a key regulator of dynamic events at the PM. In real-time assays, we find that unsaturated phosphatidylserine (PS) and sterols, ...

      Known for Osh Proteins | Synthesis Phosphatidylinositol | Membrane Lipid | Saccharomyces Cerevisiae | 45 Diphosphate

      Key People For Autophagosome Formation

      Top KOLs in the world
      Noboru N Mizushima
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      Daniel J Klionsky
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      Takeshi Noda
      endothelial cells autophagosome formation endoplasmic reticulum
      Yoshinori Ohsumi
      saccharomyces cerevisiae autophagosome formation vacuolar membrane
      Beth Levine
      beclin 1 cell death sindbis virus

      PRESTO, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0076, Japan | Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan | Molecular Cell Biology of Aut

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