• Disease
  • Interstitial
  • Interstitial Cells
  • Gianrico F Farrugia

    Prominent publications by Gianrico F Farrugia

    KOL Index score: 19358

    BACKGROUND & AIMS: In gastrointestinal muscles, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) is predominantly expressed by interstitial cells of Cajal (ICC) and platelet-derived growth factor receptor-α (PDGFRA) polypeptide is expressed by so-called fibroblast-like cells. KIT and PDGFRA have been reported to be coexpressed in ICC precursors and gastrointestinal stromal tumors (GISTs), which originate from the ICC lineage. PDGFRA signaling has been proposed to ...

    Also Ranks for: Kit Pdgfra |  growth factor |  tumor cell |  gastrointestinal stromal |  platelet derived
    KOL Index score: 17942

    Myenteric plexus interstitial cells of Cajal (ICC-MY) in the small intestine are Kit+ electrical pacemakers that express the Ano1/TMEM16A Ca2+-activated Cl- channel, whose functions in the gastrointestinal tract remain incompletely understood. In this study, an inducible Cre-LoxP-based approach was used to advance the understanding of Ano1 in ICC-MY of adult mouse small intestine. KitCreERT2/+;Ano1Fl/Fl mice were treated with tamoxifen or vehicle, and small intestines (mucosa free) were ...

    Also Ranks for: Slow Waves |  small intestine |  interstitial cells |  cajal icc |  ca2 transients
    KOL Index score: 14201

    KEY POINTS: The gastrointestinal epithelial enterochromaffin (EC) cell synthesizes the vast majority of the body's serotonin. As a specialized mechanosensor, the EC cell releases this serotonin in response to mechanical forces. However, the molecular mechanism of EC cell mechanotransduction is unknown. In the present study, we show, for the first time, that the mechanosensitive ion channel Piezo2 is specifically expressed by the human and mouse EC cells. Activation of Piezo2 by ...

    Also Ranks for: Mechanical Forces |  mechanosensitive ion channel |  enterochromaffin cell |  5ht release |  piezo2 currents
    KOL Index score: 14165

    BACKGROUND: Ion channels play important roles in regulation of cellular proliferation. Ano1 (TMEM16A) is a Ca(2+)-activated Cl(-) channel expressed in several tumors and cell types. In the muscle layers of the gastrointestinal tract Ano1 is selectively expressed in interstitial cells of Cajal (ICC) and appears to be required for normal gastrointestinal slow wave electrical activity. However, Ano1 is expressed in all classes of ICC, including those that do not generate slow waves ...

    Also Ranks for: Cell Proliferation |  cl− channel |  icc ano1 |  small mice mice |  smooth muscle
    KOL Index score: 13981

    BACKGROUND & AIMS: Interstitial cells of Cajal (ICC) are required for normal gastrointestinal motility. Loss of ICC is associated with several motility disorders. The mechanisms modulating ICC survival and proliferation are poorly understood. This study aimed to establish whether 5-hydroxytryptamine (5-HT) plays a role in regulating ICC proliferation.

    METHODS: Expression of 5-HT receptor mRNA was investigated in muscle strips, in purified populations of ICC, and in identified single ...

    Also Ranks for: Ht2b Receptor |  interstitial cells |  proliferation icc |  serotonin 5 |  motility disorders
    KOL Index score: 13957

    Populations of interstitial cells of Cajal (ICC) are altered in several gastrointestinal neuromuscular disorders. ICC are identified typically by ultrastructure and expression of Kit (CD117), a protein that is also expressed on mast cells. No other molecular marker currently exists to independently identify ICC. The expression of ANO1 (DOG1, TMEM16A), a Ca(2+)-activated Cl(-) channel, in gastrointestinal stromal tumors suggests it may be useful as an ICC marker. The aims of this study ...

    Also Ranks for: Kit Ano1 |  interstitial cells |  gastrointestinal tract |  cajal icc |  muscularis propria
    KOL Index score: 13918

    BACKGROUND & AIMS: Gastrointestinal stromal tumors (GIST) are related to interstitial cells of Cajal (ICC) and often contain activating stem cell factor receptor (Kit) or platelet-derived growth factor receptor alpha (Pdgfra) mutations. Kit/Pdgfra inhibitors such as imatinib mesylate have increased progression-free survival in metastatic GIST but are not curative. In mouse models we investigated whether Kit(low) ICC progenitors could represent an inherently Kit/Pdgfra inhibitor-resistant ...

    Also Ranks for: Stem Cells |  gastrointestinal stromal |  growth factor |  nude mice |  cajal icc
    KOL Index score: 13661

    Mutations in 11 genes that encode ion channels or their associated proteins cause inherited long QT syndrome (LQTS) and account for approximately 75-80% of cases (LQT1-11). Direct sequencing of SNTA1, the gene encoding alpha1-syntrophin, was performed in a cohort of LQTS patients that were negative for mutations in the 11 known LQTS-susceptibility genes. A missense mutation (A390V-SNTA1) was found in a patient with recurrent syncope and markedly prolonged QT interval (QTc, 530 ms). SNTA1 ...

    Also Ranks for: Macromolecular Complex |  long qt |  sodium channel |  membrane proteins |  disease genotype humans
    KOL Index score: 13080

    BACKGROUND: Normal gastrointestinal motility requires intact networks of interstitial cells of Cajal (ICC). Interstitial cells of Cajal numbers are maintained by a balance between cell loss factors and survival/trophic/growth factors. Activation of 5-HT(2B) receptors expressed on ICC increases ICC proliferation in vitro. It is not known whether 5-HT(2B) receptors on ICC are activated in vivo. The aims of this study were to investigate if adult ICC proliferate, whether the proliferation ...

    Also Ranks for: Interstitial Cells |  ht2b receptor |  icc networks |  serotonin 5 |  myenteric plexus
    KOL Index score: 12872

    In eukaryotic cells, DNA replication proceeds with continuous synthesis of leading-strand DNA and discontinuous synthesis of lagging-strand DNA. Here we describe a method, eSPAN (enrichment and sequencing of protein-associated nascent DNA), which reveals the genome-wide association of proteins with leading and lagging strands of DNA replication forks. Using this approach in budding yeast, we confirm the strand specificities of DNA polymerases delta and epsilon and show that the PCNA ...

    Also Ranks for: Replication Forks |  lagging strands |  protein binding |  pcna unloading |  strand dna
    KOL Index score: 12608

    Interstitial cells of Cajal (ICC) are pacemaker cells that generate electrical activity to drive contractility in the gastrointestinal tract via ion channels. Ano1 (Tmem16a), a Ca(2+)-activated Cl(-) channel, is an ion channel expressed in ICC. Genetic deletion of Ano1 in mice resulted in loss of slow waves in smooth muscle of small intestine. In this study, we show that Ano1 is required to maintain coordinated Ca(2+) transients between myenteric ICC (ICC-MY) of small intestine. First, ...

    Also Ranks for: Interstitial Cells |  ca2 transients |  cajal icc |  small intestine |  ano1 loss
    KOL Index score: 12297

    Interstitial cells of Cajal play a central role in the control of gastrointestinal motility. The mechanisms of communication between interstitial cells of Cajal and smooth muscle cells are to a large extent unknown. This article reviews the potential role of carbon monoxide as a messenger molecule between interstitial cells of Cajal and gastrointestinal smooth muscle cells. The machinery required for the formation of carbon monoxide is present in interstitial cells of Cajal and ...

    Also Ranks for: Interstitial Cells |  carbon monoxide |  heme oxygenase |  smooth muscle |  gastrointestinal motility
    KOL Index score: 12277

    BACKGROUND: Depletion of interstitial cells of Cajal (ICC) is associated with several gastrointestinal (GI) motility disorders. Changes in ICC networks are usually detected by immunolabeling for the receptor tyrosine kinase Kit. Ano1 (DOG1 or TMEM16A) was recently described as a marker of ICC in GI tract. Our aim was to determine whether Ano1 immunoreactivity can be used as a reliable marker for ICC in tissues from patients with motility disorders.

    METHODS: Four tissues from patients ...

    Also Ranks for: Slow Transit Constipation |  interstitial cells |  cajal icc |  kit ano1 |  motility disorders
    KOL Index score: 11781

    Gut microbiota alterations have been described in several diseases with altered gastrointestinal (GI) motility, and awareness is increasing regarding the role of the gut microbiome in modulating GI function. Serotonin [5-hydroxytryptamine (5-HT)] is a key regulator of GI motility and secretion. To determine the relationship among gut microbes, colonic contractility, and host serotonergic gene expression, we evaluated mice that were germ-free (GF) or humanized (HM; ex-GF colonized with ...

    Also Ranks for: Fatty Acids |  gut microbes |  enterochromaffin cells |  colonic serotonin |  hm mice
    KOL Index score: 11762

    BACKGROUND & AIMS: SCN5A encodes the α-subunit of the voltage-gated sodium channel NaV1.5. Many patients with cardiac arrhythmias caused by mutations in SCN5A also have symptoms of irritable bowel syndrome (IBS). We investigated whether patients with IBS have SCN5A variants that affect the function of NaV1.5.

    METHODS: We performed genotype analysis of SCN5A in 584 persons with IBS and 1380 without IBS (controls). Mutant forms of SCN5A were expressed in human embryonic kidney-293 cells, ...

    Also Ranks for: Sodium Channel |  bowel syndrome |  patients ibs |  nav15 function |  voltage gated


    Gianrico F Farrugia: Influence Statistics

    Sample of concepts for which Gianrico F Farrugia is among the top experts in the world.
    Concept World rank
    cajal loss #1
    function interstitial cells #1
    gastric emptying icc #1
    effects potassium current #1
    ano1 positivity #1
    ranolazine constipation #1
    canine jejunum effects #1
    decyclizing intestines #1
    piibs cohort #1
    v1 2a #1
    sfproducing fibroblasts icc #1
    mechanosensory epithelial #1
    mechanosensitive cells mechanoreceptors #1
    cajal protooncogene proteins #1
    icc gastric #1
    kit‐positive interstitial cells #1
    demb remb procedure #1
    remb procedure demb #1
    piezo2 gsmtx4 #1
    immunoreactivity reliable marker #1
    source canine jejunum #1
    tmem16a sites #1
    transmembrane movement iccs #1
    hco3 electrical #1
    factors muscularis macrophage #1
    upregulated mirnas rtqpcr #1
    tmem16a structure function #1
    csf1 delayed gastric #1
    icc diabetic #1
    motility diseases #1
    ano1 imparts #1
    diabetic gastroparesis damage #1
    cells gastrointestinal motility #1
    aim muscularis macrophages #1
    virtual icc #1
    cajal intestines #1
    vsics #1
    pkcgamma transgenic morpholines #1
    ho1 gastric #1
    mechanosensitivity expressed channel #1
    translation position #1
    f1760a nav15 #1
    iccs nbce1 #1
    cajal thiolesterase adult #1
    thickness gastric biopsies #1
    gastrointestinal sodium channelopathy #1
    aging icc #1
    ano1 interstitial cells #1
    nbce1 activity effects #1
    signaling molecules h2s #1

    Key People For Interstitial Cells

    Top KOLs in the world
    Kenton M Sanders
    interstitial cells slow waves smooth muscle
    Sean M Ward
    interstitial cells slow waves smooth muscle
    Jan Dirk Huizinga
    interstitial cells smooth muscle motor patterns
    Lars Thuneberg
    interstitial cells small intestine smooth muscle
    Shigeko Torihashi
    interstitial cells smooth muscle canine proximal colon
    Hanne Birte Mikkelsen
    interstitial cells muscularis externa small intestine

    Gianrico F Farrugia:Expert Impact

    Concepts for whichGianrico F Farrugiahas direct influence:Interstitial cells,  Diabetic gastroparesis,  Smooth muscle,  Gastric emptying,  Idiopathic gastroparesis,  Gastrointestinal tract,  Carbon monoxide,  Patients gastroparesis.

    Gianrico F Farrugia:KOL impact

    Concepts related to the work of other authors for whichfor which Gianrico F Farrugia has influence:Interstitial cells,  Gut microbiota,  Irritable bowel syndrome,  Nitric oxide,  Smooth muscle,  Enteric nervous,  Gastrointestinal tract.



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    Mayo Clinic Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA | Department of Gastroenterology, School of Medicine, Mayo Clinic, Rochester, Minnesota. | Mayo Clinic, Rochester, MN | Division of Gastroenterology and Hepatology, M