• Disease
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  • Spinal Cord
  • Clifford J Woolf

    Prominent publications by Clifford J Woolf

    KOL Index score: 21221

    Molecular mechanisms underlying C-fiber stimulation-induced ERK (extracellular signal-regulated kinase) activation in dorsal horn neurons and its contribution to central sensitization have been investigated. In adult rat spinal slice preparations, activation of C-fiber primary afferents by a brief exposure of capsaicin produces an eightfold to 10-fold increase in ERK phosphorylation (pERK) in superficial dorsal horn neurons. The pERK induction is reduced by blockade of NMDA, ...

    Also Ranks for: Protein Kinase |  erk activation |  dorsal horn neurons |  response element |  spraguedawley receptors
    KOL Index score: 20741

    1. Peripheral inflammation is associated with the local production of neuroactive inflammatory cytokines and growth factors. These may contribute to inflammatory pain and hyperalgesia by directly or indirectly altering the function or chemical phenotype of responsive primary sensory neurones. 2. To investigate this, inflammation was produced by the intraplantar injection of complete Freund's adjuvant (CFA) in adult rats. This resulted in a significant elevation in interleukin-1 beta ...

    Also Ranks for: Inflammatory Hyperalgesia |  ngf levels |  nonsteroidal behavior |  inflamed tissue |  cfa inflammation
    KOL Index score: 18656

    The possible involvement of p38 mitogen-activated protein kinase activation in spinal cord and dorsal root ganglion (DRG) cells in the development of peripheral neuropathic pain has been explored. Ligation of the L5 spinal nerve (SNL) on one side in adult rats produces an early onset and long-lasting mechanical allodynia. This lesion results in activation of p38 in the L5 segment of the spinal cord, most prominently in the ipsilateral dorsal horn, starting soon after the lesion (<1 d) ...

    Also Ranks for: Neuropathic Pain |  spinal cord |  p38 mitogen |  activated protein |  dorsal root ganglion
    KOL Index score: 16377

    1. Pain hypersensitivity is characterized by an increase in the response to noxious stimuli (hyperalgesia) and a reduction in threshold such that innocuous stimuli begin to elicit pain (allodynia). These sensitivity changes can be produced by an increase in excitability of dorsal horn neurons; the phenomenon of central sensitization. We have now examined whether a reduction in local segmental inhibitory mechanisms produces similar changes. The model system used for studying touch-evoked ...

    Also Ranks for: Central Sensitization |  glycine receptors |  spinal cord |  evoked allodynia |  sural nerve
    KOL Index score: 16369

    Activation of ERK (extracellular signal-regulated kinase) MAP (mitogen-activated protein) kinase in dorsal horn neurons of the spinal cord by peripheral noxious stimulation contributes to short-term pain hypersensitivity. We investigated ERK activation by peripheral inflammation and its involvement in regulating gene expression in the spinal cord and in contributing to inflammatory pain hypersensitivity. Injection of complete Freund's adjuvant (CFA) into a hindpaw produced a persistent ...

    Also Ranks for: Kinase Activation |  spinal cord |  pain hypersensitivity |  erk map |  persistent inflammatory
    KOL Index score: 16178

    Transganglionic transport of wheatgerm agglutinin conjugated horse-radish peroxidase (WGA-HRP) was used to reveal the central distribution of terminals of primary afferent fibers from peripheral nerves innervating the hind leg of the rat. In separate experiments the sizes and locations of cutaneous peripheral receptive fields were determined by electrophysiological recording techniques for each of the nerves that had been labeled with WGA-HRP. By using digital image analysis, the sizes ...

    Also Ranks for: Dorsal Horn |  afferent terminals |  somatotopic organization |  rat spinal cord |  peroneal nerve
    KOL Index score: 15780

    Here we studied the role of signaling through ErbB-family receptors in interactions between unmyelinated axons and non-myelinating Schwann cells in adult nerves. We generated transgenic mice that postnatally express a dominant-negative ErbB receptor in non-myelinating but not in myelinating Schwann cells. These mutant mice present a progressive peripheral neuropathy characterized by extensive Schwann cell proliferation and death, loss of unmyelinated axons and marked heat and cold pain ...

    Also Ranks for: Schwann Cell |  erbb receptor |  unmyelinated axons |  transgenic microscopy |  oncogene proteins
    KOL Index score: 15592

    Excitatory amino acids (EAA) acting on N-methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors play an important role in synaptic transmission in the spinal cord. Quantitative autoradiography and physiological experiments suggest that NMDA receptors are localized mainly in lamina II while kainate and AMPA receptors are found on both dorsal and ventral horn neurons. However the cell types expressing EAA receptors and their ...

    Also Ranks for: Spinal Cord |  amino acid |  ampa receptors |  neurons expressing |  cobalt uptake
    KOL Index score: 14960

    Peripheral neuropathic pain is produced by multiple etiological factors that initiate a number of diverse mechanisms operating at different sites and at different times and expressed both within, and across different disease states. Unraveling the mechanisms involved requires laboratory animal models that replicate as far as possible, the different pathophysiological changes present in patients. It is unlikely that a single animal model will include the full range of neuropathic pain ...

    Also Ranks for: Animal Model |  peripheral neuropathic pain |  nerve injury |  tight ligation |  partial denervation
    KOL Index score: 14939

    To clarify whether inhibitory transmission in the superficial dorsal horn of the spinal cord is reduced after peripheral nerve injury, we have studied synaptic transmission in lamina II neurons of an isolated adult rat spinal cord slice preparation after complete sciatic nerve transection (SNT), chronic constriction injury (CCI), or spared nerve injury (SNI). Fast excitatory transmission remains intact after all three types of nerve injury. In contrast, primary afferent-evoked IPSCs are ...

    Also Ranks for: Superficial Dorsal Horn |  nerve injury |  spinal cord |  selective loss |  spraguedawley receptors
    KOL Index score: 14865

    The somatotopic organization of A- and C-afferent fibre terminals in the dorsal horn of the rat lumbar spinal cord was compared with the spatial location of second-order dorsal horn neuronal mechanoreceptive fields. The central terminal fields of the sural, saphenous, and tibial nerve were mapped by labelling the nerves with horseradish peroxidase (HRP). A previous study used the transganglionic transport of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) to produce ...

    Also Ranks for: Somatotopic Organization |  receptive fields |  dorsal horn |  spinal cord |  afferent terminals
    KOL Index score: 14609

    BACKGROUND: Nociceptive-selective local anesthesia is produced by entry of the permanently charged lidocaine-derivative QX-314 into nociceptors when coadministered with capsaicin, a transient receptor potential vanilloid 1 (TRPV1) channel agonist. However, the pain evoked by capsaicin before establishment of the QX-314-mediated block would limit clinical utility. Because TRPV1 channels are also activated by lidocaine, the authors tested whether lidocaine can substitute for capsaicin to ...

    Also Ranks for: Lidocaine Qx314 |  nociceptive blockade |  sodium channel |  nociceptors capsaicin |  permanently charged
    KOL Index score: 14309

    1. Peripheral inflammation is characterized by heightened pain sensitivity. This hyperalgesia is the consequence of the release of inflammatory mediators, cytokines and growth factors. A key participant is the induction of the neurotrophin nerve growth factor (NGF) by interleukin-1 beta (IL-1 beta). 2. Tumour necrosis factor alpha (TNF alpha) has been shown both to produce hyperalgesia and to upregulate IL-1 beta. We have now examined whether the induction of TNF alpha in inflammatory ...

    Also Ranks for: Inflammatory Hyperalgesia |  tnf alpha |  growth factor |  tumour necrosis |  intraplantar injection
    KOL Index score: 14282

    When adult dorsal root ganglion cells are dissociated and maintained in vitro, both the small dark and the large light neurons show increases in the growth-associated protein GAP-43, a membrane phosphoprotein associated with neuronal development and plasticity. Immunoreactivity for GAP-43 appears in the cytoplasm of the cell bodies as early as 3.5 h post axotomy and is present in neurites and growth cones as soon as they develop. At early stages of culture (4 h to eight days) ...

    Also Ranks for: Dorsal Horn |  nerve injury |  ganglion cells |  gap43 immunoreactivity |  rat spinal cord
    KOL Index score: 14277

    Activation of extracellular signal-regulated kinase (ERK), a mitogen activated-protein kinase (MAPK), in dorsal horn neurons contributes to inflammatory pain by transcription-dependent and -independent means. We have now investigated if ERK is activated in the spinal cord after a spinal nerve ligation (SNL) and if this contributes to the neuropathic pain-like behavior generated in this model. An L5 SNL induces an immediate (<10 min) but transient (<6 h) induction of phosphoERK (pERK) ...

    Also Ranks for: Microglia Astrocytes |  mechanical allodynia |  pain rats |  perk neurons |  nerve ligation


    Clifford J Woolf: Influence Statistics

    Sample of concepts for which Clifford J Woolf is among the top experts in the world.
    Concept World rank
    ketamine esophageal #1
    dawley touch #1
    reciprocal interactions neurones #1
    stimulation intact animals #1
    cumulative vrp #1
    cough mice nociceptors #1
    pbr protein expression #1
    potentiating spinal #1
    bhrp dorsal horn #1
    pain protection gch1 #1
    treatment mechanical allodynia #1
    lamina peripheral axotomy #1
    esophageal hypersensitivity acid #1
    distribution active form #1
    pge2induced currents #1
    neurons preparations #1
    cumulative vrp frequency #1
    slow potentials windup #1
    intraplantar ngf ngf #1
    pbf units #1
    ventral horn drg11 #1
    responses membrane potential #1
    peripheral sensitization increase #1
    reproductive organs immunohistochemistry #1
    skin female hair #1
    knockout neurons runx1 #1
    inhibiting cough #1
    pain tissue inflammation #1
    dragon bmp coreceptor #1
    sciatic lymph node #1
    partial peripheral #1
    2fold cut #1
    lamina dorsal horn #1
    hand windup ratio #1
    firing zones #1
    denervation motor endplate #1
    anova ketamine #1
    role dorsal horn #1
    spinal humans mice #1
    activation neonatal rat #1
    preganglionic behavior #1
    endplates denervated zone #1
    perk spinal microglia #1
    bradykinin pka #1
    48 neonatal axotomy #1
    receptor protein smad5 #1
    ketamine esophageal acid #1
    airway sensory neurons #1
    lidocaine nociceptive block #1
    controls brain responses #1

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    Clifford J Woolf:Expert Impact

    Concepts for whichClifford J Woolfhas direct influence:Spinal cord,  Dorsal horn,  Neuropathic pain,  Central sensitization,  Sensory neurons,  Nerve injury,  Chronic pain,  Pain hypersensitivity.

    Clifford J Woolf:KOL impact

    Concepts related to the work of other authors for whichfor which Clifford J Woolf has influence:Neuropathic pain,  Spinal cord,  Nerve injury,  Dorsal horn,  Central sensitization,  Sensory neurons,  Mechanical allodynia.



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    F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Boston, MA 02115, USA | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA | FM Kirby Neurobiology Center, Boston Children's Hospital, Boston, Massachusetts, US. | Bos