Limestone massifs with a high density of dolines form important karst aquifers in most of the Alps, often with groundwater circulating through deep karst conduits and water coming out of closely spaced springs with flow rates of over some cubic meters per second. Although several hydrogeological studies and tracing experiments were carried out in many of these carbonate mountains in the past, the hydrogeology of most of these karst aquifers is still poorly known. Geological, ...

The pattern and time course of reactive oxygen species (ROS) production were monitored histochemically in vivo in tobacco (Nicotiana tabacum L.) cv. Bel W3 and cv. Bel B leaves, comparing the hypersensitive (HR)-like reaction induced by the abiotic stressor ozone and the response to tobacco mosaic virus (TMV). The applied assays (nitro blue tetrazolium, NBT; 2’,7’-dichlorofluorescin diacetate, DCFH-DA; 3,3’-diaminobenzidine, DAB) gave positive results, evidencing scattered ROS production ...