Prominent publications by John I Gallin

KOL Index score: 15235

Fletcher factor-deficient plasma is deficient in prekallikrein and therefore generates no bradykinin upon activation with kaolin. It also possesses a diminished rate of kaolin-activable coagulation and fibrinolysis and possesses a defect in kaolin-activable chemotactic activity. These abnormalities are also corrected by reconstitution with purified prekallikrein. Addition of intact activated Hageman factor corrected the coagulation, fibrinolytic, and chemotactic defects and addition of ...

Known for Hageman Factor |  Kinin Generation |  Chemotactic Activity |  Coagulation Fibrinolysis |  Prekallikrein Abnormalities
KOL Index score: 14174

Considerable evidence suggests that Ca2+ modulates endothelial cell metabolic and morphologic responses to mediators of inflammation. We have used the fluorescent Ca2+ indicator, quin2, to monitor endothelial cell cytosolic free Ca2+, [Ca2+]i, in cultured human umbilical vein endothelial cells. Histamine stimulated an increase in [Ca2+]i from a resting level of 111 +/- 4 nM (mean +/- SEM, n = 10) to micromolar levels; maximal and half-maximal responses were elicited by 10(-4) M and 5 X ...

Known for Cytosolic Calcium |  Albumin Diffusion |  Histamine Receptors |  Cultured Endothelial |  Sustained Elevation
KOL Index score: 14073

The kinetics of complement-related chemotactic factor generation in human serum were evaluated. Addition of immune complexes to serum resulted in formation of chemotactic activity within 5 min of incubation while activation with S. typhosa endotoxin of serum from normal subjects resulted in a prolonged (15–20 min) latent period prior to generation of chemotactic activity. In contrast to S. typhosa, endotoxin incubation of serum with E. coli endotoxin resulted in rapid formation of ...

Known for Chemotactic Factor |  Human Serum |  Complement Pathway |  Immune Complexes |  Kinetic Analysis
KOL Index score: 13489

OBJECTIVE: To describe the genotypes, phenotypes, immunophenotypes, and treatments of PAPA syndrome (pyogenic sterile arthritis, pyoderma gangrenosum, and acne), a rare autoinflammatory disease, in 5 patients.

METHODS: Clinical information was gathered from medical records and through interviews with 5 patients from 4 kindreds. PSTPIP1 (CD2BP1) exon 10 and exon 11 sequencing was performed in each patient. Neutrophil granule content and cytokine levels were determined in plasma and ...

Known for Papa Syndrome |  Pyoderma Gangrenosum |  Pyogenic Sterile Arthritis |  Cutaneous Manifestations |  5 Patients
KOL Index score: 12673

Orientation of nucleus, centriole, microtubules, and microfilaments within human neutrophils in a gradient of chemoattractant (5 percent Escherichia coli endotoxin-activated serum) was evaluated by electron microscopy. Purified neutropils (hypaque-Ficoll) were placed in the upper compartment of chemotactic chambers. Use of small pore (0.45 mum) micropore filters permitted pseudopod penetration, but impeded migration. Under conditions of chemotaxis with activated serum beneath the filter, ...

Known for Neutrophil Migration |  Microtubules Microfilaments |  Pseudopod Formation |  Activated Serum |  Nucleus Chemotaxis
KOL Index score: 12316

BACKGROUND: Aspergillus fumigatus is the most common etiologic agent of invasive aspergillosis in immunocompromised patients. Several studies have addressed the mechanism involved in host defense but only few have investigated the pathogen's response to attack by the host cells. To our knowledge, this is the first study that investigates the genes differentially expressed in conidia vs hyphae of A. fumigatus in response to neutrophils from healthy donors as well as from those with ...

Known for Aspergillus Fumigatus |  Conidia Hyphae |  Fungal Genes |  Differentially Expressed |  Host Defense
KOL Index score: 12088

Previous studies using membrane potential sensitive probes have provided evidence that chemotactic factors elicit membrane potential changes in normal human neutrophils (PMN). In addition to stimulation of PMN motility, chemotactic factors also stimulate degranulation and superoxide ion (O-2) generation and it has been suggested that alteration of membrane potential activates these events (Korchak, H. M., and G. Weissmann. 1978. Proc, Natl, Acad, Sci. U. S. A. 75: 3818--3822). To further ...

Known for Membrane Potential |  Chronic Granulomatous Disease |  Patients Cgd |  Pma Pmn |  Human Neutrophil
KOL Index score: 11411

The role of specific granules and cytochrome b in superoxide (O(2)) release was studied by comparing the effects of three different stimuli on normal human neutrophils, neutrophils congenitally deficient in specific granules, and granule-free normal neutrophil cytoplasts. Phorbol myristate acetate (PMA) stimulated normal neutrophils to release more O(2) than did N-formylmethionylleucylphenylalanine (fMet-Leu-Phe), which stimulated greater release than the calcium ionophore A23187. ...

Known for Plasma Membrane |  Specific Granules |  Human Neutrophil |  Myristate Acetate |  O2 Release
KOL Index score: 11142

BACKGROUND: The treatment of chronic granulomatous disease with conventional allogeneic hematopoietic stem-cell transplantation carries a high risk of serious complications and death. We investigated the feasibility of stem-cell transplantation without ablation of the recipient's bone marrow.

METHODS: Ten patients, five children and five adults, with chronic granulomatous disease underwent peripheral-blood stem-cell transplantation from an HLA-identical sibling. We used a ...

Known for Nonmyeloablative Conditioning |  Chronic Granulomatous Disease |  Graft Host |  Donor Lymphocytes |  Hematopoietic Stem
KOL Index score: 10964

Patients with the hyperimmunoglobulin E and recurrent infection syndrome (HIE) characteristically have frequent skin and respiratory infections caused by Staphylococcus aureus. We have developed a set of enzyme-linked immunosorbent assays that use whole S. aureus (Wood's strain) immobilized on 0.22-micrometers filters and highly specific, affinity-purified enzyme conjugates of goat anti-human IgE, anti-human IgD, anti-human IgG, anti-human IgA, and anti-human IgM. These reagents were ...

Known for Staphylococcus Aureus |  Recurrent Infection |  Patients Hie |  Immunoglobulin Syndrome |  Serum Ige
KOL Index score: 10956

Chronic granulomatous disease, a syndrome of recurrent infections and failure of oxidative microbicidal activity in phagocytes, results from defects in the gene for one of several components of an oxidase system that can undergo activation. To determine the relative prevalence of certain of the genetic variants of this disorder, we used immunoblotting to detect two specific neutrophil cytosolic proteins of 47 and 67 kd recently shown to be required for oxidase activation. Chronic ...

Known for Chronic Granulomatous Disease |  Cytosolic Components |  Nadph Oxidase |  Cytochrome B558 |  Recurrent Infections
KOL Index score: 10831

After circulating in the vascular system a short time, polymorphonuclear leukocytes (PMN) migrate to extravascular sites in response to chemotactic stimuli. Prestimulation of PMN in vitro by secretagogues has been shown to increase their number of N-formylmethionylleucylphenylalanine (fmet-leu-phe) and complement component C3bi (CR3) receptors. We investigated whether the same phenomenon occurred in vivo, comparing characteristics of human skin chamber and guinea pig peritoneal exudate ...

Known for Chemotactic Peptide |  C3bi Receptor |  Blood Pmn |  Guinea Pig |  Leu Phe
KOL Index score: 10616

BACKGROUND: The hyper-IgE syndrome (or Job's syndrome) is a rare disorder of immunity and connective tissue characterized by dermatitis, boils, cyst-forming pneumonias, elevated serum IgE levels, retained primary dentition, and bone abnormalities. Inheritance is autosomal dominant; sporadic cases are also found.

METHODS: We collected longitudinal clinical data on patients with the hyper-IgE syndrome and their families and assayed the levels of cytokines secreted by stimulated leukocytes ...

Known for Stat3 Mutations |  Hyperige Syndrome |  Cells Patients |  Sequence Analysis |  Ige Levels


John I Gallin: Influence Statistics

Sample of concepts for which John I Gallin is among the top experts in the world.
Concept World rank
b558 deficient #1
pmn subsequent responsiveness #1
retrospective study alloimmunization #1
sgd normal amounts #1
hepatosplenomegaly pulmonary infiltrates #1
glip deletion hydrocortisone #1
07 micrograms 24 #1
confocal monocytes pmn #1
strains b5233 af293 #1
schiffmann becker #1
aqueous incubation medium #1
ionic events activation #1
cgd pmns presence #1
patient jobs syndrome #1
31d8 positive #1
neutrophil dysfunction data #1
pmnmediated host defenses #1
aspergillus ciclopirox #1
fmetleuphe neutrophils #1
normal crevicular neutrophils #1
pmn receptors #1
neutrophils 31d8 #1
pmns low concentrations #1
pulmonary reactions patients #1
crpmn #1
inhibitors granulomatous #1
pus contained10 #1
glip synthesis #1
numerous chemoattractants chenoweth #1
time labelling cells #1
widespread granuloma formation #1
62 micrograms 24 #1
superoxide production interferongamma #1
glipr glipdelta cfs #1
relationship chemotactic factors #1
hydrogen peroxide interleukin8 #1
frank impetiginous lesions #1
hyperimmunoglobulin #1
normal cgd #1
chronic perirectal abscess #1
igg eac3bigg #1
secretagogue modulation #1
fumigatus messenger spores #1
laea synthesis #1
lactoferrin pmn supernatants #1
pmn normal #1
excessive inflammatory reactions #1
produced leu #1
pmns sodium azide #1
neutrophil membrane events #1

Key People For Chronic Granulomatous Disease

Top KOLs in the world
John I Gallin
chronic granulomatous disease membrane potential human neutrophils
Harry L Malech
chronic granulomatous disease gene therapy stem cells
John T Curnutte
chronic granulomatous disease nadph oxidase human neutrophils
Paul G Quie
polymorphonuclear leukocytes staphylococcus aureus chronic granulomatous disease
Stephen M Holland
chronic granulomatous disease gata2 deficiency nadph oxidase
Dirk Roos
chronic granulomatous disease human neutrophils respiratory burst

John I Gallin:Expert Impact

Concepts for whichJohn I Gallinhas direct influence:Chronic granulomatous disease,  Granulomatous disease,  Chronic granulomatous,  Membrane potential,  Human neutrophils,  Patients cgd,  Granulibacter bethesdensis,  Nadph oxidase.

John I Gallin:KOL impact

Concepts related to the work of other authors for whichfor which John I Gallin has influence:Chronic granulomatous disease,  Nadph oxidase,  Human neutrophils,  Aspergillus fumigatus,  Gene therapy,  Endothelial cells,  Staphylococcus aureus.



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Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA;,, (K.A.Z.);,, (L.J.B.);,,

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