Prominent publications by Stuart H Yuspa

KOL Index score: 21100

The expression of an oncogenic rasHa gene in epidermal keratinocytes stimulates the tyrosine phosphorylation of protein kinase C delta and inhibits its enzymatic activity (Denning, M. F., Dlugosz, A. A., Howett, M. K., and Yuspa, S. H. (1993) J. Biol. Chem. 268, 26079-26081). Keratinocytes expressing an activated rasHa gene secrete transforming growth factor alpha (TGFalpha) and have an altered response to differentiation signals involving protein kinase C (PKC). Because the neoplastic ...

Known for Tyrosine Phosphorylation |  Growth Factor |  Protein Kinase |  Pkcdelta Keratinocytes |  Signal Transduction
KOL Index score: 14948

Previously, we have shown that nuclear extracts from cultured mouse keratinocytes induced to differentiate by increasing the levels of extra-cellular calcium contain Fra-1, Fra-2, Jun B, Jun D and c-Jun proteins that bind to the AP-1 DNA binding sequence. Despite this DNA binding activity, AP-1 reporter activity was suppressed in these cells. Here, we have detected the CREB family proteins CREB and CREMα as additional participants in the AP-1 DNA binding complex in differentiating ...

Known for Transcriptional Activity |  Binding Protein |  Differentiating Keratinocytes |  Factor Creb |  Ap1 Cre
KOL Index score: 14814

PURPOSE: CLIC4, a member of a family of intracellular chloride channels, is regulated by p53, c-Myc, and tumor necrosis factor-alpha. Regulation by factors involved in cancer pathogenesis, together with the previously shown proapoptotic activity of CLIC4, suggests that the protein may have a tumor suppressor function. To address this possibility, we characterized the expression profile, subcellular localization, and gene integrity of CLIC4 in human cancers and determined the functional ...

Known for Clic4 Expression |  Tumor Cells |  Neoplastic Genes |  Multiple Human Cancers |  Alphasmooth Muscle Actin
KOL Index score: 14580

Mouse skin carcinomas arise from a small subpopulation of benign papillomas with an increased risk of malignant conversion. These papillomas arise with limited stimulation by tumor promoters, appear rapidly, and do not regress, suggesting that they differ in growth properties from the majority of benign tumors. The transforming growth factor beta (TGF-beta) proteins are expressed in the epidermis and are growth inhibitors for mouse keratinocytes in vitro; altered TGF-beta expression ...

Known for Malignant Conversion |  Skin Tumors |  Factor Beta |  Transforming Growth |  Loss Expression
KOL Index score: 14397

Skin keratinocytes are subject to frequent chemical and physical injury and have developed elaborate cell survival mechanisms to compensate. Among these, the Akt/protein kinase B (PKB) pathway protects keratinocytes from the toxic effects of ultraviolet light (UV). In contrast, the protein kinase C (PKC) family is involved in several keratinocyte death pathways. During an examination of potential interactions among these two pathways, we found that the insulin-like growth factor (IGF-1) ...

Known for Protein Kinase |  Akt Activity |  Insulinlike Growth Factor |  Induced Apoptosis |  Cell Survival
KOL Index score: 14259

Specific keratin cDNA probes and monospecific antikeratin antisera were used to analyze mouse epidermis and epidermal tumors for the expression of a type I 47-kDa keratin, K13, normally associated with terminal differentiation of internal stratified epithelia. We demonstrated that this keratin was virtually absent from the entire body epidermis at various stages of development. Also, it was not detected in various forms of acute and chronic epidermal hyperproliferation or in epidermal ...

Known for Terminal Differentiation |  Aberrant Expression |  Internal Stratified Epithelia |  Mouse Skin |  Tumor Carcinoma
KOL Index score: 13832

Epidermal keratinocyte differentiation is a tightly regulated, stepwise process that requires protein kinase C (PKC) activation. Studies using cultured mouse keratinocytes induced to differentiate with Ca2+ have indirectly implicated the alpha isoform of PKC in upregulation of "late" (granular cell) epidermal differentiation markers. Activation of this isoform is also implicated in the suppression of "early" differentiation markers keratin (K) 1 and 10 that characterizes the neoplastic ...

Known for Pkc Alpha |  Antisense Oligonucleotides |  Differentiation Markers |  Epidermal Keratinocytes |  Protein Kinase
KOL Index score: 13374

Epidermal differentiation is characterized by a series of coordinated morphological and biochemical changes which result in a highly specialized, highly organized, stratified squamous epithelium. Among the specific markers expressed in differentiating epidermis are (a) two early spinous cell proteins, keratins 1 and 10 (K1 and K10); and (b) two later granular cell proteins, filaggrin and a cornified envelope precursor (CE). In vitro, epidermal basal cells are selectively cultured in 0.05 ...

Known for Differentiation Markers |  Calcium Concentrations |  Marker Expression |  Ca2 Medium |  Newborn Blotting
KOL Index score: 13243

In order to determine whether pemphigold antigen is synthesized by epidermal cells, and whether other, normal keratinocyte, antigens are synthesized in culture, primary cultures and subsequent subcultures of human epidermal cells derived from neonatal foreskins were studied for the presence of pemphigoid antigen, pemphigus antigen and keratin filaments using indirect immunofluorescence. Twenty-four hr after plating primary cultures, pemphigoid antigen was detected as an asymmetric ...

Known for Pemphigoid Antigen |  Epidermal Cells |  Indirect Immunofluorescence |  Cultured Human |  Primary Cultures
KOL Index score: 13198

The protein kinase C (PKC) family of phospholipid-dependent serine-threonine kinases has been implicated in keratinocyte differentiation and neoplastic transformation. To determine if Ca(2+)-mediated keratinocyte differentiation is associated with changes in PKC isozyme gene expression, RNA was isolated from primary mouse keratinocytes grown in medium with 0.05, 0.12, or 1.4 mM Ca2+. Based on northern blot analysis, primary keratinocytes expressed mRNA encoding PKC-alpha, -delta, ...

Known for Neoplastic Cells |  Protein Kinase |  Normal Keratinocytes |  Ras Gene |  Cell Differentiation


Stuart H Yuspa: Influence Statistics

Sample of concepts for which Stuart H Yuspa is among the top experts in the world.
Concept World rank
treatment urethane #1
weeks tpa #1
marker expression markers #1
keratinocytes increased #1
cultured mouse keratinocytes #1
formation intrastrand diammineplatinum #1
promotion 01 μg #1
ing3a pma #1
papilloma phenotype #1
induction corticosteroids #1
tpa treatment stage #1
fca tpa #1
tgk tpa #1
calcium antiserum #1
antibody rat filaggrin #1
reciprocal modifications #1
stsp signaling pathway #1
diamminedichloroplatinumiidna #1
normal epidermal cells #1
005 mm keratinocytes #1
decarboxylase anesthetics #1
clic4 tgfβ signaling #1
squamous tumors #1
bapta cai #1
pkcdeltaadv overexpression #1
proliferation collagenase #1
dermis studies #1
latent tumor phenotype #1
recognizes soluble keratins #1
hair follicles grafts #1
targeted loss selenoproteins #1
mice mice afos #1
cells pkc eta #1
human k1 gene #1
vharas keratinocytes #1
elevating clic4 #1
epidermal tumors contribution #1
uvc tpa #1
fibroblasts cdna arrays #1
p53 mtclic #1
assay abcb1 inhibitors #1
keratinocytes lactones #1
systemic neutrophilia pkcalpha #1
akt activity tpa #1
domes phenylalanine mutants #1
melanoma ing3a #1
vrasha keratinocytes #1
staurosporine stsp #1
risk papillomas #1
k13 terminal differentiation #1

Key People For Epidermal Cells

Top KOLs in the world
Howard A Green
adipose conversion involucrin gene 3t3 cells
Elaine V Fuchs
stem cells intermediate filaments transgenic mice
Stuart H Yuspa
epidermal cells protein kinase malignant conversion
Stephen I Katz
langerhans cells basement membrane dermatitis herpetiformis
Fiona M Watt
stem cells terminal differentiation proton beam
Georg Stingl
langerhans cells atopic dermatitis inbred balb mice

Stuart H Yuspa:Expert Impact

Concepts for whichStuart H Yuspahas direct influence:Epidermal cells,  Protein kinase,  Terminal differentiation,  Mouse skin,  Malignant conversion,  Retinoic acid,  Growth factor,  Neoplastic cells.

Stuart H Yuspa:KOL impact

Concepts related to the work of other authors for whichfor which Stuart H Yuspa has influence:Protein kinase,  Growth factor,  Retinoic acid,  Gene expression,  Epithelial cells,  Human keratinocytes,  Cell proliferation.



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In Vitro Pathogenesis Section, Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA | Laboratory of Cancer Biology and Genetics, Center for Cancer Res

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