• KOL
    • Filter Paper
    • Enzyme-Linked...
    • Enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.: Influence Statistics

      Expert Impact

      Concepts for whichthey havehas direct influence:Filter paper,17 alpha,Linked immunosorbent,Immunosorbent assay,Dried blood,Mass screening,Enzyme conjugate,17 alpha 17ohp.

      Key People For Filter Paper

      Top KOLs in the world
      #1
      William H Hannon
      newborn screening united states tandem mass spectrometry
      #2
      Jiang Jiang
      ion transport failure mechanism gas bubbles
      #3
      R Guthrie
      newborn infant cord blood filter paper
      #4
      George M Whitesides
      soft lithography microcontact printing charge transport
      #5
      Joanne V Mei
      united states newborn screening disease control
      #6
      Barbara W Adam
      newborn screening united states tyrosinemia type

      Enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.

      Abstract

      In this rapid, cost-effective, enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone (17-OHP) eluted from dried blood spotted on filter paper, second antibody is coated onto the microwell plate and horseradish peroxidase (EC 1.11.1.7) is the label enzyme. Antiserum to 17-OHP was prepared by using 4-(2-carboxymethylthio)-17-OHP-bovine serum albumin conjugate as immunogen. Enzyme conjugate was prepared from 4-(2-carboxymethylthio)-17-OHP and peroxidase. The blood spots are assayed in the microwells without extraction or centrifugation steps. The detection limit of the assay is 1 microgram/L, equivalent to 3.5 pg (10.6 fmol) per disc. Intra- and interassay CVs at two steroid concentrations (7.38 and 22.79 micrograms/L) ranged from 3.74 to 11.90% (n = 5), and 9.49 and 9.83% (n = 5), respectively. Results correlated well (r = 0.91) with those of a fluorescence enzyme immunoassay. The sensitivity, specificity, and precision of this method make it potentially useful in the mass screening of neonates for congenital adrenal hyperplasia.

      Sign-in to see all concepts, it's free!

       

    Download on the App StoreGet it on Google Play

    Copyright © 2023 Key Opinion Leaders, LLC.