Prominent publications by James Michael Wilson

KOL Index score: 16587

Lentiviral vectors have proven to be promising tools for transduction of central nervous system (CNS) cells in vivo and in vitro. In this study, CNS transduction patterns of lentiviral vectors pseudotyped with envelope glycoproteins from Ebola virus, murine leukemia virus (MuLV), lymphocytic choriomeningitis virus (LCMV), or the rabies-related Mokola virus were compared to a vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G). Mokola-, LCMV-, and VSV-G-pseudotyped ...

Known for Mouse Brain |  Lentivirus Vectors |  Targeted Transduction |  Envelope Proteins |  Lcmv Mulv
KOL Index score: 15303

Coronary artery disease (CAD) is the leading cause of morbidity and mortality in patients with chronic kidney disease (CKD), but no study has yet compared the short- and long-term outcomes of coronary artery bypass grafting (CABG) and percutaneous coronary intervention (PCI) with drug-eluting stents for multivessel CAD among non-hemodialysis-dependent (HD) patients with CKD. In our institution's registry, we identified 812 patients with CKD (glomerular filtration rate <60 ml/min) who had ...

Known for Coronary Artery |  Cabg Pci |  Kidney Disease |  Multivessel Cad |  Drugeluting Stents
KOL Index score: 14628

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated, ATP-gated Cl(-) channel and cellular conductance regulator, but the detailed mechanisms of CFTR regulation and its regulation of other transport proteins remain obscure. We previously identified the metabolic sensor AMP-activated protein kinase (AMPK) as a novel protein interacting with CFTR and found that AMPK phosphorylated CFTR and inhibited CFTR-dependent whole cell conductances when coexpressed with ...

Known for Cftr Activity |  T84 Cells |  Protein Kinase |  Polarized Epithelia |  Fluorescent Antibody
KOL Index score: 14559

BACKGROUND: Cystic fibrosis is a monogenic disease that deranges multiple systems of ion transport in the airways, culminating in chronic infection and destruction of the lung. The introduction of a normal copy of the cystic fibrosis transmembrane conductance regulator (CFTR) gene into the airway epithelium through gene transfer is an attractive approach to correcting the underlying defects in patients with cystic fibrosis. We tested the feasibility of gene therapy using adenoviral ...

Known for Gene Transfer |  Cystic Fibrosis |  Nasal Epithelium |  Adenoviral Vector |  Ion Transport
KOL Index score: 14540

BACKGROUND: Atrial fibrillation (AF) after cardiac surgery is associated with significant morbidity. We investigated whether preoperative statin therapy was associated with decreased incidence of postoperative AF in patients undergoing cardiac surgery, including isolated valve surgery and patients with low ejection fraction (EF).

METHODS: A retrospective study of consecutive patients without history of AF (n = 4044) who underwent cardiac surgeries at St. Luke's Episcopal Hospital ...

Known for Preoperative Statin Therapy |  Cardiac Surgery |  Atrial Fibrillation |  Postoperative Patients |  Decreased Risk
KOL Index score: 14245

Recombinant adenoviruses have tremendous potential for gene therapy of cystic fibrosis (CF) lung disease. First-generation recombinant viruses, rendered replication defective by deleting E1, have been associated with high-level recombinant gene expression in airway epithelial cells when administered directly to the lung. Experience in mice and non-human primates indicates that transgene expression is transient (i.e., lasting less than 21 days) and associated with the development of ...

Known for Transgene Expression |  Recombinant Adenoviruses |  Viral Gene |  Cystic Fibrosis |  Cd8 Cells
KOL Index score: 13848

Recombinant adenoviruses are being evaluated for gene therapy of cystic fibrosis lung disease with the goal of reconstituting the expression of the cystic fibrosis transmembrane conductance regulator in pulmonary epithelia by direct administration of the virus into the airway. The therapeutic potential of recombinant adenoviruses is limited in part by the relative inefficiency by which gene transfer occurs. This study uses a human bronchial xenograft model to study adenovirus infection ...

Known for Gene Transfer |  Human Airway |  Recombinant Adenoviruses |  Vitronectin Recombination |  Cystic Fibrosis
KOL Index score: 13504

The successful application of gene therapy for the treatment of genetic diseases such as Fabry is reliant on the development of vectors that are safe and that facilitate sustained expression of therapeutic levels of the transgene product. Here, we report that intravenous administration of a recombinant AAV2 vector encoding human alpha-galactosidase A under the transcriptional control of a liver-restricted enhancer/promoter (AAV2/DC190-alphagal) generated significantly higher levels of ...

Known for Fabry Mice |  Immune Tolerance |  Therapeutic Levels |  Animal Enhancer Elements |  Aav2 Vector
KOL Index score: 12956

Toll-like receptors (TLRs) have been implicated in the regulation of host responses to microbial Ags. This study characterizes the role of TLR4 in the innate immune response to intrapulmonary administration of Haemophilus influenzae in the mouse. Two different strains of mice efficiently cleared aerosolized H. influenzae concurrent with a brisk elaboration of IL-1beta, IL-6, TNF-alpha, macrophage-inflammatory protein (MIP)-1alpha, and MIP-2 in bronchoalveolar lavage and a corresponding ...

Known for Immune Responses |  Mouse Lung |  Receptor 4 |  Haemophilus Influenzae |  Bronchoalveolar Lavage
KOL Index score: 12636

Pseudomonas aeruginosa, an opportunistic respiratory pathogen that infects the majority of patients with cystic fibrosis, initiates host inflammatory responses through interaction with airway epithelial cells. The Toll-like receptors (TLRs) are a family of pathogen pattern recognition receptors that play key roles in host innate immunity. In this study we aimed to determine whether TLRs mediate the interaction between P. aeruginosa and airway epithelial cells. Individual murine TLRs ...

Known for Epithelial Cells |  Pseudomonas Aeruginosa |  Receptor 5 |  Human Airway |  Tlr1 Tlr2
KOL Index score: 12613

RATIONALE: Familial hypercholesterolemia is a genetic disorder that arises because of loss-of-function mutations in the low-density lipoprotein receptor (LDLR) and homozygous familial hypercholesterolemia is a candidate for gene therapy using adeno-associated viral vectors. Proprotein convertase subtilisin/kexin type 9 (PCSK9) and inducible degrader of LDLR (IDOL) negatively regulate LDLR protein and could dampen adeno-associated viral vector encoded LDLR expression.

OBJECTIVE: We sought ...

Known for Familial Hypercholesterolemia |  Mouse Model |  Ldlr Idol |  Pcsk9 Hpcsk9 |  Cholesterol Levels
KOL Index score: 12481

Immune responses to adenovirus-mediated gene transfer contribute to the problems of transient recombinant gene expression, inflammation, and difficulties with vector readministration. Activation of CD4+ T cells is required for full realization of effector function of both CD8+ T cells (i.e., cytotoxic T cells) and B cells (i.e., neutralizing antibody). We evaluate in this study the effectiveness of a short course of high-dose cyclophosphamide to block immune responses in mice ...

Known for Transgene Expression |  Mouse Liver |  Adenoviral Vectors |  Neutralizing Antibody |  Immune Responses
KOL Index score: 12257

Malignant pleural mesothelioma is a fatal neoplasm that is unresponsive to standard modalities of cancer therapy. We conducted a phase I dose-escalation clinical trial of adenoviral (Ad)-mediated intrapleural herpes simplex virus thymidine kinase (HSVtk)/ganciclovir (GCV) gene therapy in patients with mesothelioma as a model for treatment of a localized malignancy. The goals of this phase I trial were to assess the safety, toxicity, and maximally tolerated dose of intrapleural Ad.HSVtk, ...

Known for Malignant Mesothelioma |  Thymidine Kinase |  Localized Malignancy |  Ganciclovir Gene |  Simplex Virus
KOL Index score: 12239

E1-deleted adenoviral recombinants most commonly based on the human serotype 5 (AdHu5) have been shown thus far to induce unsurpassed transgene product-specific CD8(+) T cell responses. A large percentage of the adult human population carries neutralizing Abs due to natural exposures to AdHu5 virus. To circumvent reduction of the efficacy of adenovirus (Ad) vector-based vaccines by neutralizing Abs to the vaccine carrier, we developed E1-deleted adenoviral vaccine carriers based on ...

Known for Adenoviral Vaccine |  Secondary Injections |  Prime Boost |  Transgene Product |  Adhu5 Virus


James Michael Wilson: Influence Statistics

Sample of concepts for which James Michael Wilson is among the top experts in the world.
Concept World rank
natural aav infections #1
aav infections #1
minimally effective dose #1
dose vector #1
persisting vector genome #1
preclinical toxicology study #1
genetic transgenes alpha #1
spf ash mice #1
viral drug administration #1
toxicity drg neurons #1
hvr viii #1
tregs kcs #1
variety animal models #1
kcs immune responses #1
e4orf6 overexpression #1
process enzymatic rescue #1
adeno aav #1
aav based #1
trastuzumab prevents #1
mps elevated spermine #1
adenovirus dope #1
aavadenovirus #1
ad5cbcftr ad5cmv lacz #1
combination tlr1 #1
expression levels vectors #1
liver vectors #1
transduction foot #1
salt mucus #1
avb sepharose #1
monoclonal antibody aav9 #1
major neutralizing site #1
ittreated dogs #1
combination adenovirus #1
serotyping transgenes #1
vectors aav3b #1
instillation virus #1
treatment mut #1
pseudotyped lentiviral vectors #1
human cd18 #1
vector encoded #1
primate aavs #1
hvrs vp3 #1
complementary genomes #1
gene expression pegylation #1
adenoviruses gene #1
lta stimulation hbd2promoterluc #1
poaf isolated cabg #1
vivo vector sequences #1
pao1 aeruginosa strain #1
raav human factor #1

Key People For Gene Therapy

Top KOLs in the world
James Michael Wilson
gene therapy cystic fibrosis aav vectors
Mark A Kay
gene transfer mouse liver aav vectors
Richard Jude Samulski
gene transfer aav vectors adenoassociated virus
Katherine A High
gene transfer factor vii aav capsid
Luigi M Naldini
gene therapy lentiviral vectors stem cell
Inder M Verma
lentiviral vectors gene therapy fos protein

James Michael Wilson:Expert Impact

Concepts for whichJames Michael Wilsonhas direct influence:Gene therapy,  Gene transfer,  Cystic fibrosis,  Gene expression,  Transgene expression,  Aav vectors,  Genetic vectors,  Adenoviral vectors.

James Michael Wilson:KOL impact

Concepts related to the work of other authors for whichfor which James Michael Wilson has influence:Gene therapy,  Cystic fibrosis,  Viral vectors,  Antimicrobial peptides,  Atrial fibrillation,  Epithelial cells,  Animal models.



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Gene Therapy Program, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA | Gene Therapy Program, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelph

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