Frank L Graham

Frank L Graham

Department of Biology, McMaster University, Hamilton, Ontario, Canada | Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada | ...

KOL Resume for Frank L Graham  (profound mental retardation, emotional, retardation, mental, behavioral, emotional behavioral disorders, disorders, mental retardation)

Year
2012

Department of Biology, McMaster University, Hamilton, Ontario, Canada

2010

Department of Pathology and Molecular Medicine, McMaster University, 1200 Main Street West, Hamilton, ONT, L8N 3Z5, Canada

2006

Department of Biology, McMaster University, Life Sciences Building, Room 430, Hamilton, Ontario, L8S 4K1, Canada

2005

Departments of Biology and Pathology and Molecular Medicine, McMaster University, 1200 Main Street West, Hamilton, ON, Canada L8N 3Z5; and

2004

Departments of Biology, and Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

2003

Department of Pathology, McMaster University, Hamilton, Ontario, Canada

2002

Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada L8S 4K1

Department of Biology, McMaster University Hamilton, Ontario, Canada

2001

Departments of Biology and Pathology, McMaster University, Hamilton, Ontario, Canada

2000

Biology, Centre for Gene Therapeutics, McMaster University, Hamilton, Ont.,

Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 7011 Thurston-Bowles Building, CB# 7248, 27599-7248, Chapel Hill, North Carolina, USA

McMaster University, Hamilton, ON, Canada

Departments of Pathology and Molecular Medicine and

1999

Departments of Biology, McMaster University, Hamilton, Ontario, Canada

1998

McMaster University Hamilton, Ontario, Canada

Departments of Biology,

Cancer Research Group, Institute for Molecular Biology and Biotechnology, and

1997

Department of Pathology McMaster University Hamilton, Ontario, Canada L8S 4KI

Biology and

Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada.

Pathology, McMaster University, 1280 Main Street West, Hamilton, ON Canada L8S 4K1

1996

Departments of 1Biology and 2Pathology, McMaster University, Hamilton, Ontario, Canada L8S 4K1. 3Both authors have contributed equally to the work presented in this article.

Department of Biology and Pathology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1.

Hematology

Biology, McMaster University, Hamilton, Ontario, Canada

1995

From the Section of Cardiology (A.J.M., Q.-T.Y., D.L.M., R.R.), Baylor College of Medicine, Houston, Tex, and the Departments of Biology and Pathology (F.L.G.), McMaster University, Hamilton, Ontario, Canada.

Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1

1994

Departments of Biology McMaster University, Hamilton, Ont., Canada

Fred Hutchinson Cancer Research Center Seattle, Washington 98104 USA

 

Prominent publications by Frank L Graham

KOL Index score: 21194

Cell cycle regulatory proteins are important candidates for therapeutic tumour suppressors. Adenovirus vectors were constructed to overexpress cyclin kinase inhibitors p16INK4A, p18INK4C, p19INK4D, p21WAF1/CIP1 and p27KIP1 under the control of the murine cytomegalovirus immediate early gene promoter. These vectors directed the efficient expression of each of the cyclin kinase inhibitors and induced growth arrest, inhibited DNA synthesis, and prevented phosphorylation of the ...

Known for Adenovirus Vectors |  Kinase Inhibitor |  Cell Cycle |  Cyclin Dependent |  Growth Arrest
KOL Index score: 19923

Transgenic mice expressing the polyomavirus (PyV) middle T antigen (MT) develop multifocal mammary tumors which frequently metastasize to the lung. The potent transforming activity of PyV MT is correlated with its capacity to activate and associate with a number of signaling molecules, including the Src family tyrosine kinases, the 85-kDa Src homology 2 subunit of the phosphatidylinositol 3' (PI-3') kinase, and the Shc adapter protein. To uncover the role of these signaling proteins in ...

Known for Kinase Signaling |  Mammary Tumorigenesis |  Transgenic Mice |  Phosphatidylinositol 3 |  Polyomavirus Middle
KOL Index score: 14785

Inflammatory bowel disease (IBD) is characterized by altered immunoregulation and augmented intestinal synthesis of nitric oxide. The purpose of this study was to determine the effects of exogenous IL-4, introduced by a recombinant human type 5 adenovirus (Ad5) vector, on the tissue injury associated with an experimental model of colonic immune activation and inflammation. Colitis was induced in rats by the intrarectal administration of trinitrobenzene sulfonic acid (TNB) dissolved in ...

Known for Gene Transfer |  Inflammatory Bowel |  Ad5il4 Ad5lacz |  Nitric Oxide |  Distal Colon
KOL Index score: 14162

The cloning capacity of currently available E1- and E3-deleted adenovirus (Ad) vectors does not exceed 8 kb. To increase capacity and improve vector safety further, we have explored the possibility that Early Region 4 (E4) and the gene encoding protein IX (pIX) might also be deleted. To generate cell lines expressing sufficient levels of E4 and pIX proteins in trans in addition to E1-encoded proteins to complement mutations in these genes, we transformed 293 cells with constructs ...

Known for Cell Lines |  E4 Proteins |  Defective Adenovirus |  Viral Dna |  Vectors E1
KOL Index score: 13786

Tumor necrosis factor-alpha is up-regulated in a variety of different human immune-inflammatory and fibrotic pulmonary pathologies. However, its precise role in these pathologies and, in particular, the mechanism(s) by which it may induce fibrogenesis are not yet elucidated. Using a replication-deficient adenovirus to transfer the cDNA of tumor necrosis factor-alpha to rat lung, we have been able to study the effect of transient but prolonged (7 to 10 days) overexpression of tumor ...

Known for Growth Factor |  Tumor Necrosis |  Rat Lung |  Gene Transfer |  Precise Role
KOL Index score: 12518

We have developed a number of replication defective adenoviral (Ad) vectors which express transgenes under the control of the human cytomegalovirus (HCMV) immediate early (IE) gene promoter. The orientation of the expression cassette replacing E1 in the vector backbone had a significant effect on the level of transgene expression, with vectors containing expression cassettes directed towards the right end of the Ad genome expressing 7-fold higher levels of beta-galactosidase (beta-gal) ...

Known for Transgene Expression |  Adenoviral Vectors |  Hcmv Promoter |  Murine Cytomegalovirus |  Early Gene
KOL Index score: 11672

Adenoviruses are attractive vectors for the delivery of foreign genes into mammalian cells for gene therapy. However, current vectors retain many viral genes that, when expressed at low levels, contribute to the induction of a host immune response against transduced cells. We have developed a helper-dependent packaging system for production of vectors that have large regions of the genome deleted. Helper viruses were constructed with packaging signals flanked by loxP sites so that in 293 ...

Known for Helper Virus |  Packaging Signal |  Vector Genome |  Viral Genes |  Dna Replication
KOL Index score: 11661

The adenovirus 5 early region 1A (ElA: 0–4.5 map units) proteins synthesized early after infection of KB cells without pretreatment of the cells with metabolic inhibitors have been studied. The E1A mRNA species of 1.1 and 0.9 kilobases (Kb) are processed from alternative 5′ splice sites to a common 3′ splice site of the same initial transcript and code for proteins of 289 and 243 amino acids in length that share common amino and carboxy terminal peptides but differ in that 46 residues of ...

Known for E1a Proteins |  Human Adenovirus Type |  Early Region 1a |  Messenger Rna |  Carboxy Terminus
KOL Index score: 11551

The infection of epithelia] swine testicle and intestinal porcine epithelial (IPEC-1) cell lines by adenovirus type 5 (Ad5) has been studied in vitro by using an Ad5-luciferase recombinant containing the firefly luciferase gene as a reporter. Porcine cell lines supported Ad5 replication, showing virus titers, kinetics of virus production, and luciferase expression levels similar to those obtained in human 293 cells, which constitutively express the 5'-end 11% of the Ad5 genome. The ...

Known for Transmissible Gastroenteritis |  Adenovirus Type |  Ad5 Vectors |  Epithelial Cells |  Viral Vaccines
KOL Index score: 11546

We have studied the ability of adenoviral (Ad) vectors expressing the cytokines IL-2 or IL-12 to mediate regression of established tumors in a mouse model of mammary adenocarcinoma. Previous results indicated that intratumoral injection of vectors expressing IL-2 (AdCAIL-2) or IL-12 (AdmIL-12.1) induced complete tumor regression in approximately 30–40% of treated animals. In the current studies, we investigated the mechanism of tumor killing in responding animals and the efficacy of ...

Known for Adenoviral Vectors |  Experimental Mice Mice |  Il2 Il12 |  Treated Animals |  Combination Treatment
KOL Index score: 11387

DNA of human adenoviruses 2 and 5 was cleaved by the restriction endonucleases HsuI, BamHI, HpaI, and SmaI. The resulting fragments were separated and tested for their ability to transform primary baby rat kidney (BRK) cells, using the calcium technique. Fragments with transforming activity were obtained with endo's R·EcoRI (fragments A), BamHI (fragments B of Ad2 and A of Ad5 DNA), and HsuI (fragments G). The transforming fragments all represented the left terminal fragments of the ...

Known for Transforming Activity |  5 Dna |  Viral Genes |  Human Antigens |  Adenovirus 2
KOL Index score: 11153

Stimulation of antitumor immune mechanisms is the primary goal of cancer immunotherapy, and accumulating evidence suggests that effective alteration of the host-tumor relationship involves immunomodulating cytokines and also the presence of costimulatory molecules. To examine the antitumor effect of direct in vivo gene transfer of murine interleukin 12 (IL-12) and B7-1 into tumors, we developed an adenovirus (Ad) vector, AdIL12-B7-1, that encodes the two IL-12 subunits in early region 1 ...

Known for Tumor Regression |  B7 1 |  Experimental Mice Mice |  Mammary Neoplasms |  Adenovirus Vector

 

Frank L Graham: Influence Statistics

Sample of concepts for which Frank L Graham is among the top experts in the world.
Concept World rank
hrinfected cultures #1
vivo recombinant dystrophin #1
single itr shuttle #1
long psa promoter #1
transcription units e1a #1
plasmid containingloxp #1
dna transformed lines #1
hsv glycoproteins virions #1
ads bacterial plasmids #1
mammalian cells characteristics #1
57 insertion events #1
ad5 dna hsui #1
endocytosis transduced ids #1
efficient promoter mcmv #1
homology ad5 #1
vp7sc vaccine #1
histidinol resistant cells #1
infectious herpesvirus dna #1
double recombinant #1
gene therapy dl309 #1
recombinant bcv #1
bav3 proteins adenovirus #1
adt7pol1 adt7pol2 #1
replication adgb2 #1
transformation cultured cells #1
adpsaptk #1
fragment transformed #1
848 result #1
viral neoplasms #1
o1 aprt activity #1
non‐immunogenic reporter gene #1
highlevel protein expression #1
2 techniques characterization #1
deaedextran technique #1
adhh12b7 #1
hdexpressing rep78 #1
adenoviralbased expression #1
vectors tumour #1
adrg4 #1
19k antigen #1
m6p receptors mprs #1
trentin rabson #1
vectors t7 #1
surface enhancer elements #1
galactosidase adenoviruses #1
ad5mcmvel #1
tgevneutralizing antibodies #1
antigen promoters #1
genes viral cells #1
region immunodominant site #1

Key People For Gene Transfer

Top KOLs in the world
#1
James Michael Wilson
gene therapy cystic fibrosis aav vectors
#2
Ronald G Crystal
gene transfer alveolar macrophages cystic fibrosis
#3
A Dusty Miller
gene transfer retroviral vectors jaagsiekte sheep retrovirus
#4
Mark A Kay
gene transfer mouse liver aav vectors
#5
Richard C Mulligan
gene transfer stem cells bone marrow
#6
Inder M Verma
lentiviral vectors gene therapy fos protein

Frank L Graham:Expert Impact

Concepts for whichFrank L Grahamhas direct influence:Gene transfer,  Adenovirus vectors,  Adenoviral vectors,  Gene expression,  Gene therapy,  Adenovirus type,  Human adenovirus,  Adenovirus vector.

Frank L Graham:KOL impact

Concepts related to the work of other authors for whichfor which Frank L Graham has influence:Gene therapy,  Cell lines,  Pulmonary fibrosis,  Simplex virus,  Growth factor,  Adenoviral vectors,  Viral dna.


 

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Department of Biology, McMaster University, Hamilton, Ontario, Canada | Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada | Department of Pathology and Molecular Medicine, McMaster University, 1200 Main St

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