Prominent publications by H Maurice Goodman

KOL Index score: 14767

The 5′-end of the genome of avian sarcoma virus (ASV) contains a sequence of nucleotides which is the initial template for transcription by RNA-directed DNA polymerase. We have prepared DNA complementary to this sequence (cDNA5,) and have characterized the sequence by chemical analysis and molecular hybridization. The length of cDNA5, (00 nucleotides) provides an estimate of the distance from the primer for DNA synthesis (tRNAtrp) to the 5′-terminus of the genome. As reported previously ...

Known for Dna Complementary |  Nucleotide Sequence |  Sarcoma Virus |  Viral Transcription |  Asv Genome
KOL Index score: 13358

Comparative genome studies are important contributors to our understanding of genome evolution. Most comparative genome studies in plants have been based on genetic mapping of homologous DNA loci in different genomes. Large-scale comparative physical mapping has been hindered by the lack of efficient and affordable techniques. We report here the adaptation of fluorescence in situ hybridization (FISH) techniques for comparative physical mapping between Arabidopsis thaliana and Brassica ...

Known for Situ Hybridization |  Rapa Genome |  Bacterial Artificial |  Comparative Fluorescence |  Arabidopsis Thaliana
KOL Index score: 12651

Synthesis of the endogenous opioid precursor, proenkephalin, is regulated by neurotransmitters and membrane depolarization. These events act through second messenger dependent signal transduction pathways via a short inducible DNA enhancer to regulate transcription of the proenkephalin gene. Two DNA elements located within this enhancer are essential for the transcriptional response to cAMP and phorbol ester. Inactivation of either element by mutation or by alteration of their ...

Known for Proenkephalin Camp |  Dna Elements |  Enkcre1 Enkcre2 |  Transcription Factors |  Genetic Humans
KOL Index score: 12419

Glutamine synthetase (GS) plays an important role in the assimilation of nitrogen by higher plants. We present here a molecular analysis of the GS polypeptides, mRNAs, and genes of Arabidopsis thaliana. Western blot analysis of leaf and root protein extracts revealed at least two distinct GS polypeptides; 43 kDa and 39 kDa GS polypeptides were present in leaves, while only a 39 kDa GS was detected in roots. The 43 kDa GS polypeptide is light-inducible. In etiolated seedlings only the 39 ...

Known for Arabidopsis Thaliana |  Northern Blotting |  Leaves Roots |  Glutamine Synthetase |  39 Kda
KOL Index score: 12163

Transcription of DNA from the RNA genome of avian sarcoma virus by RNA-directed DNA polymerase in vitro initiates on a primer (tRNATrp) located near the 5'-terminus of the viral genome. One of the major products of transcription is a single-stranded DNA chain complementary to a sequence of 101 nucleotides immediately distal to the site of initiation of DNA synthesis. We have determined the complete nucleotide sequence of this transcribed chain for the Prague strain of avian sarcoma ...

Known for Avian Sarcoma Virus |  Nucleotide Sequence |  Viral Rna |  Initiation Dna Synthesis |  Directed Dna
KOL Index score: 11620

We report here the identification of a cis-acting region involved in light regulation of the nuclear gene (GapB) encoding the B subunit of chloroplast glyceraldehyde 3-phosphate dehydrogenase from Arabidopsis thaliana. Our results show that a 664-bp GapB promoter fragment is sufficient to confer light induction and organ-specific expression of the Escherichia coli beta-glucuronidase reporter gene (Gus) in transgenic tobacco (Nicotiana tabacum) plants. Deletion analysis indicates that the ...

Known for Nuclear Gene |  Arabidopsis Thaliana |  Gap Boxes |  Light Induction |  Phosphate Dehydrogenase
KOL Index score: 11559

Supercoiled DNA molecules were used for the molecular cloning of full-length avian sarcoma virus (ASV) DNA. Viral DNA produced by the Schmidt-Ruppin A (SR-A) strain of ASV was isolated from acutely infected transformed quail cells. Supercoiled DNA was separated from linear and open circular DNA by acid phenol extraction, opened into a full-length linear form by cleavage with the restriction endonuclease SacI, and cloned into lambda gtWES x lambda B. Four different cloned viral DNA ...

Known for Molecular Cloning |  Circular Dna |  Terminal Redundancy |  Avian Sarcoma |  Src Gene
KOL Index score: 11272

Growth hormone (GH) produces insulin-like effects in rat adipocytes that have been deprived of GH for at least 3 h. The effect of a saturating concentration of GH is qualitatively and quantitatively similar to that produced by 2-4 ng/ml insulin but differs from that of insulin in the respect that adipocytes become refractory to prolonged or repeated stimulation with GH. Since activation of tyrosine kinase is an early event in the action of both hormones, we investigated the possibility ...

Known for Tyrosine Phosphorylation |  Insulin Receptor |  Growth Hormone |  Data Irs1 |  Beta Subunit
KOL Index score: 11264

The fusion protein (F) gene of mumps virus was cloned from a cDNA library constructed from infected cell mRNA. The F-specific plasmids were identified by hybridization to a degenerate oligonucleotide probe whose sequence was deduced from the N-terminal amino acid sequence of the F2 protein. The complete nucleotide sequence of the F gene was determined. The gene is 1786 nucleotides long and encodes one long open reading frame of 538 amino acids. The F protein has a 19-amino acid signal ...

Known for Fusion Protein Gene |  Mumps Virus |  Messenger Rna |  Viral Sequence Homology |  Sequence Protein
KOL Index score: 11242

The major capsid protein (VP1) of simian virus 40 (SV40) has been analyzed by two-dimensional electrophoresis. This system separates protein according to isoelectric point by isoelectric-focusing, and according to molecular weight by sodium dodecylsulphate electrophoresis (O'Farrell, 1975). VP1 synthesis in infected CV-1 cells can be monitored directly by analysis of unfractionated whole cell extracts; the resolution of VP1 from cellular proteins allows its detection as early as 13 hr ...

Known for Simian Virus |  Major Capsid Protein |  Dimensional Electrophoresis |  Molecular Weight |  Isoelectric Point
KOL Index score: 11171

The enzymatic transcription of DNA from the 70S RNA of Rous sarcoma virus (RSV) is initiated on the 3' terminus of a molecule of 4S RNA which is hydrogen bonded to the viral genome. We labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70S RNA by thermal denaturation was accompanied by a reduction in the template activity of the viral RNA. Two-dimensional electrophoresis in polyacrylamide gels separated the 4S RNAs associated with the 70S RNA of ...

Known for 70s Rna |  Rous Sarcoma Virus |  Genetic Transcription |  Dna Synthesis |  Viral Genome
KOL Index score: 10302

Double-stranded DNA fragments terminated at their 5′-ends by the singlestranded sequence pA-A-T-T-, generated by digestion of DNA with EcoRI restriction endonuclease, were ligated with Escherichia coli polynucleotide ligase under various conditions of temperature, concentration and time. The linear and circular products of ligation were separated by electrophoresis in agarose gel and quantitated by densitometry. The rate of ligation of (EcoRI-cleaved) simian virus (SV40) DNA at a ...

Known for Dna Fragment |  Viral Electrophoresis |  Circular Structures |  Ecori Endonuclease |  Linear Concatemers
KOL Index score: 10295

The two nonallelic genes, insulin I and II of the rat, are separated by at least 7 kb of DNA. There is no obvious similarity in the sequence organization surrounding each gene, although the coding regions of the genes themselves share extensive homology. In three strains of rat, the insulin II gene lies predominantly on a 4.0 kb Eco RI restriction fragment, whereas the insulin I gene is located on a 9.4 kb Eco RI fragment in the Hooded strain, on a 7.2 kb Eco RI fragment in the ...

Known for Insulin Gene |  Cloned Rat |  Intervening Sequences |  Dna Sequence |  Coding Region
KOL Index score: 10248

Activation of neurotransmitter receptors can regulate transcription in postsynaptic cells through the actions of second messengers. Trans-synaptic regulation of transcription appears to be an important mechanism controlling the synthesis of molecules involved in neuronal signaling, especially neuropeptides. Proenkephalin, vasoactive intestinal polypeptide, and somatostatin have been shown to be transcriptionally regulated by the second messenger, cyclic AMP (cAMP), as has the ...

Known for Transcriptional Regulation |  Cyclic Camp |  Enkcre1 Enkcre2 |  Vasoactive Intestinal |  Transcription Factors


H Maurice Goodman: Influence Statistics

Sample of concepts for which H Maurice Goodman is among the top experts in the world.
Concept World rank
nr2 mrna #1
hemophilusinfluenzae #1
bifunctional genes protozoa #1
gh channels #1
factor sr1 #1
addition tissuespecific level #1
recombination human insulin #1
inducible enhancer activity #1
yac rflp #1
insulinlike sensitivity #1
growth hormone segments #1
ecori conditions sites #1
apetala 2 domain #1
humans plastids swine #1
arg43 species specificity #1
esherichia fragments #1
gapc 00 #1
growth hormone adipocytes #1
cruciferae characteristics #1
mutant hy1 #1
clones beta globin #1
aataataa 20 #1
cdna α #1
splice coding sequence #1
dhfr activity step #1
glt1t mutant nadhgogat #1
camp promoter regions #1
rgh gene messenger #1
enkcre1 enkcre2 #1
macromolecular substances messenger #1
rate transamination product #1
rat2 proteins #1
phosphorylase lipolysis #1
insulin produces #1
mrna fourth plasmid #1
camp enkcre1 #1
protein ankyrin repeats #1
epinephrine glycerol #1
map arabidopsis #1
plastids heme #1
dna ecori conditions #1
3terminal region strand #1
enktf1 enkcre1 sequences #1
maize viviparous1 protein #1
enktf1 enkcre2 #1
arabidopsis ispf homolog #1
medium simple radioimmunoassay #1
rat gcr #1
16k iso #1

Key People For Growth Hormone

Top KOLs in the world
Michael O Thorner
growth hormone gh secretion prolactin release
Ron G Rosenfeld
growth factor binding protein turner syndrome
Raymond L Hintz
growth factor insulin receptors binding proteins
Jens Sandahl Sandahl Christiansen
growth hormone body composition turner syndrome
BengtÅke NÅ Bengtsson†
growth hormone body composition gh replacement
David Robert Clemmons
growth factor binding protein smooth muscle

H Maurice Goodman:Expert Impact

Concepts for whichH Maurice Goodmanhas direct influence:Growth hormone,  Adipose tissue,  Rat adipocytes,  Okadaic acid,  Nucleotide sequence,  Arabidopsis thaliana,  Arabidopsis genome,  Gene expression.

H Maurice Goodman:KOL impact

Concepts related to the work of other authors for whichfor which H Maurice Goodman has influence:Growth hormone,  Gene expression,  Arabidopsis thaliana,  Escherichia coli,  Amino acid,  Nucleotide sequence,  Protein kinase.



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University of California, Berkeley | Department of Psychology, University of Massachusetts Medical School | Department of Molecular Biology, Massachusetts General Hospital, 02114, Boston, MA, USA | Department of Genetics, Harvard Medical School, 0211

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