Prominent publications by Richard B Presland

KOL Index score: 10774

Flaky tail (gene symbol ft) is an autosomal recessive mutation in mice that results in a dry, flaky skin, and annular tail and paw constrictions in the neonatal period. Previous studies demonstrated that the ft mutation maps to the central region of mouse chromosome 3, in the vicinity of the epidermal differentiation complex, a gene locus that includes many nonkeratin genes expressed in epidermis. In this study we report a detailed characterization of the flaky tail mouse. Affected ...

Also Ranks for: Flaky Tail |  animal model |  ichthyosis vulgaris |  profilaggrin filaggrin |  skin disease
KOL Index score: 10645

Filaggrin is an intermediate filament-associated protein which functions to aggregate keratin intermediate filaments in the stratum corneum of mammalian epidermis. It is synthesized as a large precursor protein, profilaggrin, that consists of multiple filaggrin units and is localized in keratohyalin granules. In this report, we describe the characterization of cosmid genomic clones containing the human profilaggrin gene coding for 11 complete filaggrin repeats of 324 amino acids each. At ...

Also Ranks for: Calcium Binding |  amino terminus |  profilaggrin filaggrin |  cultured cloning |  sequence homology
KOL Index score: 9938

Filaggrin is an intermediate filament (IF)-associated protein that aggregates keratin IFs in vitro and is thought to perform a similar function during the terminal differentiation of epidermal keratinocytes. To further explore the role of filaggrin in the cytoskeletal rearrangement that accompanies epidermal differentiation, we generated keratinocyte cell lines that express human filaggrin using a tetracycline-inducible promoter system. Filaggrin expression resulted in reduced ...

Also Ranks for: Cell Adhesion |  human filaggrin |  epidermal keratinocytes |  intermediate filament |  terminal differentiation
KOL Index score: 9706

Epithelial tissues function to protect the organism from physical, chemical, and microbial damage and are essential for survival. To perform this role, epithelial keratinocytes undergo a well-defined differentiation program that results in the expression of structural proteins which maintain the integrity of epithelial tissues and function as a protective barrier. This review focuses on structural proteins of the epidermis and oral mucosa. Keratin proteins comprise the predominant ...

Also Ranks for: Structural Proteins |  oral cavity |  epithelial differentiation |  skin diseases |  keratin cytoskeleton
KOL Index score: 9684

BACKGROUND: Extracellular calcium is an important regulator of keratinocyte differentiation. An increase in intracellular calcium ion concentration is required for activation of calcium-induced keratinocyte differentiation. The signaling elements in this differentiation response include the calcium sensing receptor, phospholipase C, release of calcium ions from intracellular stores, and store-operated calcium channels. Nothing is currently known about the calcium-entry channels activated ...

Also Ranks for: Trpc Channels |  induced differentiation |  gingival keratinocytes |  channel expression |  extracellular calcium
KOL Index score: 9656

Profilaggrin is a large phosphoprotein that is expressed in the granular cells of epidermis where it is localized in keratohyalin. It consists of multiple copies of single filaggrin units plus N- and C-terminal sequences that differ from filaggrin. Profilaggrin is dephosphorylated and proteolytically processed during terminal differentiation to yield filaggrin, which associates with keratin intermediate filaments to form macrofibrils in the lower layers of the stratum corneum. The ...

Also Ranks for: Epidermal Differentiation |  terminal domain |  human profilaggrin |  stratum corneum |  protein structure
KOL Index score: 8637

We describe the isolation and characterization of a set of overlapping cosmid clones that contain chicken keratin genes. The 100 kb (1 kb = 10(3) base-pairs) of DNA represented in these clones contains a cluster of 18 feather keratin genes spanning 53 kb of DNA. The feather keratin genes are spaced about 3 kb apart and at least 11 of them have the same transcriptional orientation. Southern analysis using oligonucleotide probes made from highly conserved portions of the 5' non-coding, ...

Also Ranks for: Keratin Genes |  gene families |  restriction mapping |  southern chick |  animals base
KOL Index score: 8121

Disruption of skin barrier function leads to increases in the percutaneous transfer of allergens and the incidence of atopic dermatitis. Flaky tail (Flg(ft)) mice have been used as a model of atopic dermatitis with skin barrier dysfunction. Although Flg(ft) mice are known to have filaggrin mutation, the mechanism responsible for the skin barrier dysfunction that they display needs to be determined, especially for the roles of epidermal adhesion and junction proteins. Herein, we report ...

Also Ranks for: Growth Factor |  skin flgft mice |  atopic dermatitis |  flaky tail |  junction proteins
KOL Index score: 8019

Profilaggrin is a large phosphorylated protein (approximately 400 kDa in humans) that is expressed in the granular cells of epidermis where it forms a major component of keratohyalin. It consists of multiple copies of similar filaggrin units plus amino- and carboxy-terminal domains that differ from filaggrin. Proteolytic processing of profilaggrin during terminal differentiation results in the removal of these domains and generation of monomeric filaggrin units, which associate with ...

Also Ranks for: Human Profilaggrin |  binding proteins |  terminal differentiation |  sequence calcium |  amino terminus
KOL Index score: 7928

Specific proteolysis plays an important role in the terminal differentiation of keratinocytes in the epidermis and several types of proteases have been implicated in this process. The proprotein convertases (PCs) are a family of Ca2+-dependent serine proteases involved in processing and activation of several types of substrates. In this study we examined the expression and some potential substrates of PCs in epidermis. Four PCs are expressed in epidermis: furin, PACE4, PC5/6 and PC7/8. ...

Also Ranks for: Proprotein Convertases Pcs |  epidermis furin |  granular layer |  convertase expression |  terminal differentiation
KOL Index score: 7868

Filaggrin is an intermediate filament associated protein that aids the packing of keratin filaments during terminal differentiation of keratinocytes. Premature aggregation of keratin filaments is prevented by filaggrin expression as the inactive precursor, profilaggrin, which is localized in keratohyalin granules in vivo. Profilaggrin is phosphorylated and contains multiple filaggrin repeats separated by a hydrophobic linker peptide. We have previously shown that filaggrin constructs ...

Also Ranks for: Keratin Filaments |  filaggrin constructs |  keratohyalin granules |  epithelial cells |  terminal differentiation
KOL Index score: 7842

BACKGROUND: Epidermal-type transglutaminase (TGase 3) is involved in the cross-linking of structural proteins in the epidermis, which results in the formation of the cornified envelope. TGase 3 is activated by limited proteolysis of a 77 kDa zymogen during keratinocyte differentiation.

OBJECTIVE: To characterize the expression of TGase 3 in human epidermis and cultured keratinocytes, we established specific monoclonal antibodies against the TGase 3.

METHODS: Recombinant proteins for ...

Also Ranks for: Cultured Keratinocytes |  transglutaminase 3 |  human epidermis |  cornified envelope |  monoclonal antibodies
KOL Index score: 7537

Filaggrin is an intermediate filament-associated protein (IFAP) that aggregates epidermal keratin filaments in vitro and is thought to perform a similar function during terminal differentiation in vivo. To test this function in living cells, we transiently expressed constructs encoding human filaggrin in both simple epithelial cells (COS-7) and rat keratinocytes. Scanning laser confocal microscopy showed that filaggrin-positive cells had collapsed keratin and vimentin intermediate ...

Also Ranks for: Intermediate Filament |  epidermal filaggrin |  cell shape |  terminal differentiation |  nuclear integrity
KOL Index score: 7509

Caspase-14 belongs to a conserved family of aspartate-specific proteinases. Its expression is restricted almost exclusively to the suprabasal layers of the epidermis and the hair follicles1,2,3,4. Moreover, the proteolytic activation of caspase-14 is associated with stratum corneum formation, implicating caspase-14 in terminal keratinocyte differentiation and cornification5,6. Here, we show that the skin of caspase-14-deficient mice was shiny and lichenified, indicating an altered ...

Also Ranks for: Water Loss |  stratum corneum |  knockout microscopy |  mice mice |  filaggrin skin

Key People For Human Profilaggrin

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Beverly * ****
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mass spectrometry phosphorylation sites deuterium exchange
Richard * ********
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Richard B Presland:Expert Impact

Concepts for whichRichard B Preslandhas direct influence:Human profilaggrin,  Cultured keratinocytes,  Retinoic acid,  Ichthyosis vulgaris,  Keratin genes,  Structural proteins,  Buffering capacity,  Epidermal keratinocytes.

Richard B Presland:KOL impact

Concepts related to the work of other authors for whichfor which Richard B Presland has influence:Atopic dermatitis,  Skin barrier,  Stratum corneum,  Ichthyosis vulgaris,  Oral mucosa,  Food allergy,  Flg mutations.



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Oral Medicine Department, University of Washington, Seattle, WA, USA | Associate Professor and Director, Graduate Program in Oral Biology, Department of Oral Health Sciences, School of Dentistry, University of Washington, Seattle, WA, USA | Universit