Prominent publications by Raymond J Dattwyler

KOL Index score: 12611

Lyme disease is the major tick-borne disease, caused by Borrelia burgdorferi (Bb). Neurological involvement is common in all stages. In vivo expression of Bb antigens (Ags) and the immune response to them has not been well investigated in the cerebrospinal fluid (CSF). Upregulation of outer surface protein (Osp) C and concomitant downregulation of OspA before tick inoculation of the spirochete has been reported in skin and blood in animals. CSF OspA Ag in early disease suggests otherwise ...

Also Ranks for: Lyme Disease |  cerebrospinal fluid |  bacterial antigens |  csf serum |  membrane proteins
KOL Index score: 10473

Current serodiagnostic assays for Lyme disease are inadequate at detecting early infection due to poor sensitivity and nonspecificity that arise from the use of whole bacteria or bacterial proteins as assay targets; both targets contain epitopes that are cross-reactive with epitopes found in antigens of other bacterial species. Tests utilizing peptides that contain individual epitopes highly specific for Borrelia burgdorferi as diagnostic targets are an attractive alternative to current ...

Also Ranks for: Early Lyme Disease |  borrelia burgdorferi |  bacterial antigens |  mammalian infection |  linear epitopes
KOL Index score: 10260

The immunodominant proteins and glycoproteins of Borrelia burgdorferi were analyzed by one-dimensional (1D) and 2D gel electrophoresis. More than 100 polypeptide species could be detected on silver-stained 2D gels. Separation of sonic extracts of the organism by differential centrifugation (100,000 X g) revealed several of the major proteins to reside predominantly within the pellet fraction. The antigenicity of the individual polypeptides was determined by Western (immuno-) blot ...

Also Ranks for: Surface Proteins |  borrelia burgdorferi |  bacterial antigens |  amino acids |  kda protein
KOL Index score: 10125

Babesiosis is a tick-borne zoonosis caused by protozoans of the genus Babesia, apicomplexan parasites that replicate within erythrocytes. However, unlike related Plasmodium species, the pathogenesis of Babesia infection remains poorly understood. The primary etiological agent of babesiosis in the United States is B. microti. In healthy individuals, tick-transmitted infection with Babesia causes no specific clinical manifestations, with many having no symptoms at all. However, even in ...

Also Ranks for: Babesia Microti |  united states |  c3h mice |  apicomplexan parasites |  cd4 cells
KOL Index score: 9738

The current standard for laboratory diagnosis of Lyme disease in the United States is serologic detection of antibodies against Borrelia burgdorferi. The Centers for Disease Control and Prevention recommends a two-tiered testing algorithm; however, this scheme has limited sensitivity for detecting early Lyme disease. Thus, there is a need to improve diagnostics for Lyme disease at the early stage, when antibiotic treatment is highly efficacious. We examined novel and established antigen ...

Also Ranks for: Lyme Disease |  bacterial borrelia |  burgdorferi infection |  diagnostic tests |  multiantigen panel
KOL Index score: 9655

Evidence-based guidelines for the management of patients with Lyme disease, human granulocytic anaplasmosis (formerly known as human granulocytic ehrlichiosis), and babesiosis were prepared by an expert panel of the Infectious Diseases Society of America. These updated guidelines replace the previous treatment guidelines published in 2000 (Clin Infect Dis 2000; 31[Suppl 1]:1-14). The guidelines are intended for use by health care providers who care for patients who either have these ...

Also Ranks for: Lyme Disease |  granulocytic anaplasmosis |  clinical practice guidelines |  treatment prevention |  antimicrobial therapy
KOL Index score: 9430

OBJECTIVE: To determine central nervous system (CNS) involvement in acutely disseminated Borrelia burgdorferi infection by measurement of borrelia-specific DNA using the polymerase chain-reaction (PCR) assay and to compare the results of this with standard serological tests.

DESIGN: Prospective study with laboratory investigators blinded to clinical data.

SETTING: Multicenter office practice with a central reference laboratory.

PATIENTS: Cerebrospinal fluid (CSF) was collected from 12 ...

Also Ranks for: Central Nervous |  borrelia burgdorferi |  erythema migrans |  cerebrospinal fluid csf |  bacterial humans
KOL Index score: 8750

Lyme disease begins at the site of a tick bite, producing a primary infection with spread of the organism to secondary sites occurring early in the course of infection. A major outer surface protein expressed by the spirochete early in infection is outer surface protein C (OspC). In Borrelia burgdorferi sensu stricto, OspC is highly variable. Based on sequence divergence, alleles of ospC can be divided into 21 major groups. To assess whether strain differences defined by ospC group are ...

Also Ranks for: Sensu Stricto |  borrelia burgdorferi |  invasive infection |  major groups |  lyme disease
KOL Index score: 8554

BACKGROUND: Localized Lyme disease, manifested by erythema migrans, is usually treated with oral doxycycline or amoxicillin. Whether acute disseminated Borrelia burgdorferi infection should be treated differently from localized infection is unknown.

METHODS: We conducted a prospective, open-label, randomized, multicenter study comparing parenteral ceftriaxone (2 g once daily for 14 days) with oral doxycycline (100 mg twice daily for 21 days) in patients with acute disseminated B. ...

Also Ranks for: Oral Doxycycline |  lyme disease |  erythema migrans |  skin lesion |  objective evidence
KOL Index score: 8407

Yersinia pestis, one of history's deadliest pathogens, has killed millions over the course of human history. It has attributes that make it an ideal choice to produce mass casualties and is a prime candidate for use as a biological weapon. When aerosolized, Y. pestis causes pneumonic plague, a pneumonia that is 100% lethal if not promptly treated with effective antibiotics. Currently, there is no FDA approved plague vaccine. The current lead vaccine candidate, a parenterally administered ...

Also Ranks for: Pneumonic Plague |  subunit vaccine |  bacterial antigens |  tobacco mosaic |  f1 lcrv
KOL Index score: 8393

Current serologic Lyme disease tests use whole borrelia cells as the source of antigen. These assays are difficult to standardize and to optimize for sensitivity and specificity. To help solve these problems, we constructed a library of recombinant chimeric proteins composed of portions of key antigens of Borrelia burgdorferi. These proteins were then used to develop an enzyme-linked immunosorbent assay. We compared our assay with the most sensitive of three whole-cell borrelia assays. ...

Also Ranks for: Lyme Disease |  bacterial antigens |  recombinant assay |  sensitivity specificity |  borrelia proteins
KOL Index score: 8352

The major outer surface membrane protein of Borrelia burgdorferi, OspA, is one of several antigens recognized by sera from some patients in the chronic phase of Lyme borreliosis. We have expressed the OspA open reading frame in Escherichia coli and generated a series of deletion constructs of the gene and expressed them as trpE fusion proteins in E. coli. These constructs were used to identify antibody-binding sites of both rabbit antiserum and mouse monoclonal antibodies (MAbs) directed ...

Also Ranks for: Borrelia Burgdorferi |  outer surface |  binding site |  amino acids |  mabs ospa

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Raymond J Dattwyler:Expert Impact

Concepts for whichRaymond J Dattwylerhas direct influence:Lyme disease,  Borrelia burgdorferi,  Lyme borreliosis,  Bacterial antigens,  Early lyme disease,  Lymphocyte subsets,  Diagnostic tests,  Erythema migrans.

Raymond J Dattwyler:KOL impact

Concepts related to the work of other authors for whichfor which Raymond J Dattwyler has influence:Lyme disease,  Borrelia burgdorferi,  Erythema migrans,  United states,  Antibiotic treatment,  Bacterial antigens,  Tick bite.



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Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA | Biopeptides Corp., East Setauket, New York, USA | Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA | Department of Mi