Prominent publications by Hao Sun

KOL Index score: 14459

BACKGROUND: The gene regulatory information is hardwired in the promoter regions formed by cis-regulatory elements that bind specific transcription factors (TFs). Hence, establishing the architecture of plant promoters is fundamental to understanding gene expression. The determination of the regulatory circuits controlled by each TF and the identification of the cis-regulatory sequences for all genes have been identified as two of the goals of the Multinational Coordinated Arabidopsis ...

Also Ranks for: Transcription Factors |  gene regulatory |  promoter regions |  agris databases |  plant arabidopsis
KOL Index score: 13087

Massively parallel sequencing of DNA molecules in the plasma of pregnant women has been shown to allow accurate and noninvasive prenatal detection of fetal trisomy 21. However, whether the sequencing approach is as accurate for the noninvasive prenatal diagnosis of trisomy 13 and 18 is unclear due to the lack of data from a large sample set. We studied 392 pregnancies, among which 25 involved a trisomy 13 fetus and 37 involved a trisomy 18 fetus, by massively parallel sequencing. By ...

Also Ranks for: Trisomy 18 |  noninvasive prenatal diagnosis |  dna sequencing |  pregnant women |  human pair
KOL Index score: 11534

OBJECTIVES: To validate the clinical efficacy and practical feasibility of massively parallel maternal plasma DNA sequencing to screen for fetal trisomy 21 among high risk pregnancies clinically indicated for amniocentesis or chorionic villus sampling.

DESIGN: Diagnostic accuracy validated against full karyotyping, using prospectively collected or archived maternal plasma samples.

SETTING: Prenatal diagnostic units in Hong Kong, United Kingdom, and the Netherlands.


Also Ranks for: Trisomy 21 |  dna sequencing |  maternal plasma |  positive predictive |  risk pregnancies
KOL Index score: 11443

Noninvasive prenatal testing using fetal DNA in maternal plasma is an actively researched area. The current generation of tests using massively parallel sequencing is based on counting plasma DNA sequences originating from different genomic regions. In this study, we explored a different approach that is based on the use of DNA fragment size as a diagnostic parameter. This approach is dependent on the fact that circulating fetal DNA molecules are generally shorter than the corresponding ...

Also Ranks for: Plasma Dna |  noninvasive prenatal |  sensitivity specificity |  molecular diagnostics |  fetal trisomy
KOL Index score: 11401

BACKGROUND: Noninvasive prenatal diagnosis of trisomy 21 (T21) has recently been shown to be achievable by massively parallel sequencing of maternal plasma on a sequencing-by-synthesis platform. The quantification of several other human chromosomes, including chromosomes 18 and 13, has been shown to be less precise, however, with quantitative biases related to the chromosomal GC content.

METHODS: Maternal plasma DNA from 10 euploid and 5 T21 pregnancies was sequenced with a ...

Also Ranks for: Noninvasive Prenatal Diagnosis |  massively parallel sequencing |  maternal plasma dna |  trisomy 21 |  human pair
KOL Index score: 11315

BACKGROUND: Plasma DNA obtained from a pregnant woman contains a mixture of maternal and fetal DNA. The fetal DNA proportion in maternal plasma is relatively consistent as determined using polymorphic genetic markers across different chromosomes in euploid pregnancies. For aneuploid pregnancies, the observed fetal DNA proportion measured using polymorphic genetic markers for the aneuploid chromosome would be perturbed. In this study, we investigated the feasibility of analyzing single ...

Also Ranks for: Plasma Dna |  fetal trisomy |  parallel sequencing |  allelic ratio analysis |  prenatal diagnosis
KOL Index score: 11066

We performed a high-resolution analysis of the biological characteristics of plasma DNA in systemic lupus erythematosus (SLE) patients using massively parallel genomic and methylomic sequencing. A number of plasma DNA abnormalities were found. First, aberrations in measured genomic representations (MGRs) were identified in the plasma DNA of SLE patients. The extent of the aberrations in MGRs correlated with anti-double-stranded DNA (anti-dsDNA) antibody level. Second, the plasma DNA of ...

Also Ranks for: Plasma Dna |  sle patients |  lupus erythematosus |  methylation epigenesis |  healthy individuals
KOL Index score: 11033

Gene regulatory pathways converge at the level of transcription, where interactions among regulatory genes and between regulators and target genes result in the establishment of spatiotemporal patterns of gene expression. The growing identification of direct target genes for key transcription factors (TFs) through traditional and high-throughput experimental approaches has facilitated the elucidation of regulatory networks at the genome level. To integrate this information into a ...

Also Ranks for: Regulatory Networks |  transcription factors |  target genes |  arabidopsis thaliana |  promoter sequences
KOL Index score: 10687

We explored the detection of genome-wide hypomethylation in plasma using shotgun massively parallel bisulfite sequencing as a marker for cancer. Tumor-associated copy number aberrations (CNAs) could also be observed from the bisulfite DNA sequencing data. Hypomethylation and CNAs were detected in the plasma DNA of patients with hepatocellular carcinoma, breast cancer, lung cancer, nasopharyngeal cancer, smooth muscle sarcoma, and neuroendocrine tumor. For the detection of nonmetastatic ...

Also Ranks for: Plasma Dna |  cancer detection |  sensitivity specificity |  bisulfite sequencing |  copy aberrations
KOL Index score: 10578

Skeletal muscle cell differentiation (myogenesis) is a process orchestrated by a complex network involving transcription factors, epigenetic regulators, and microRNAs. Previous studies identified miR-29 as a pro-myogenic factor that interacts with components of Polycomb repressive complex, YY1 and Ezh2. In a genome-wide survey of miR-29-mediated transcriptome changes in C2C12 myoblasts, many epigenetic factors were found to be down-regulated by miR-29. Among them, Rybp was shown to be a ...

Also Ranks for: Skeletal Myogenesis |  zeste homolog |  binding protein |  transcription factors |  polycomb repressive complex
KOL Index score: 9968

MOTIVATION: The fractional fetal DNA concentration is one of the critical parameters for non-invasive prenatal diagnosis based on the analysis of DNA in maternal plasma. Massively parallel sequencing (MPS) of DNA in maternal plasma has been demonstrated to be a powerful tool for the non-invasive prenatal diagnosis of fetal chromosomal aneuploidies. With the rapid advance of MPS technologies, the sequencing cost per base is dramatically reducing, especially when using targeted MPS. Even ...

Also Ranks for: Maternal Plasma |  fetal dna |  targeted mps |  parallel sequencing |  prenatal diagnosis
KOL Index score: 9850

BACKGROUND: Fetal DNA in maternal urine, if present, would be a valuable source of fetal genetic material for noninvasive prenatal diagnosis. However, the existence of fetal DNA in maternal urine has remained controversial. The issue is due to the lack of appropriate technology to robustly detect the potentially highly degraded fetal DNA in maternal urine.

METHODOLOGY: We have used massively parallel paired-end sequencing to investigate cell-free DNA molecules in maternal urine. ...

Also Ranks for: Fetal Dna |  pregnant women |  massively parallel |  nucleotide sequencing |  size distributions
KOL Index score: 9607

BACKGROUND: Analysis of circulating RNA in the plasma of pregnant women has the potential to serve as a powerful tool for noninvasive prenatal testing and research. However, detection of circulating RNA in the plasma in an unbiased and high-throughput manner has been technically challenging. Therefore, only a limited number of circulating RNA species in maternal plasma have been validated as pregnancy- and placenta-specific biomarkers.

METHODS: We explored the use of massively parallel ...

Also Ranks for: Maternal Plasma |  rna sequencing |  pregnancy trimester |  pregnant women |  noninvasive prenatal testing
KOL Index score: 9507

BACKGROUND: Epigenetic mechanisms play an important role in prenatal development, but fetal tissues are not readily accessible. Fetal DNA molecules are present in maternal plasma and can be analyzed noninvasively.

METHODS: We applied genomewide bisulfite sequencing via 2 approaches to analyze the methylation profile of maternal plasma DNA at single-nucleotide resolution. The first approach used maternal blood samples and polymorphic differences between the mother and fetus to analyze the ...

Also Ranks for: Maternal Plasma |  bisulfite sequencing |  fetal dna |  noninvasive prenatal |  human pair
KOL Index score: 9430

Cell-free DNA (cfDNA) in human plasma is a class of biomarkers with many current and potential future diagnostic applications. Recent studies have shown that cfDNA molecules are not randomly fragmented and possess information related to their tissues of origin. Pathologies causing death of cells from particular tissues result in perturbations in the relative distribution of DNA from the affected tissues. Such tissue-of-origin analysis is particularly useful in the development of liquid ...

Also Ranks for: Chromatin Regions |  dna fragmentation |  plasma cell |  tissues origin |  liquid biopsies

Key People For Maternal Plasma

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Christopher * * ******
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Hao Sun:Expert Impact

Concepts for whichHao Sunhas direct influence:Maternal plasma,  Plasma dna,  Muscle regeneration,  Maternal plasma dna,  Massively parallel sequencing,  Skeletal myogenesis,  Transcription factors,  Fetal dna.

Hao Sun:KOL impact

Concepts related to the work of other authors for whichfor which Hao Sun has influence:Gene expression,  Skeletal muscle,  Maternal plasma,  Liquid biopsy,  Tumor dna,  Prenatal diagnosis,  Transcription factors.



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Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong 999077, China. | Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, H