Michael J. Lichten

Michael J. Lichten

Laboratory Of Biochemistry And Molecular Biology, Center For Cancer Research, National Cancer Institute, 20892-4260, Bethesda, Md, Usa Nih, Building 37, Room 6124, 37 Convent ...

Direct Impact

Concepts for which Michael J Lichten has direct influence:

meiotic recombination
saccharomyces cerevisiae
heteroduplex dna
vde-initiated crossovers
budding yeast meiosis
chromatin transition
branch migration

External impact

Concepts related to the work of other authors for which Michael J Lichten has influence:

meiotic recombination
dna damage
saccharomyces cerevisiae
srs2 helicase
late prophase
mms sensitivity
short chromosomes

Prominent publications by Michael J. Lichten

KOL-Index: 51 Currently favored models for meiotic recombination posit that both noncrossover and crossover recombination are initiated by DNA double-strand breaks but form by different mechanisms: noncrossovers by synthesis-dependent strand annealing and crossovers by formation and resolution of double Holliday junctions centered around the break. This dual mechanism hypothesis predicts different hybrid ...
Known for
Highly Dynamic Process | Dual Mechanism Hypothesis | Noncrossover Crossover | Meiotic Recombination Posit
KOL-Index: 49 Abstract Currently favored models for meiotic recombination posit that both noncrossover and crossover recombination are initiated by DNA double strand breaks but form by different mechanisms, noncrossovers by synthesis dependent strand annealing, and crossovers by formation and resolution of double Holliday junctions centered around the break. This dual mechanism hypothesis predicts ...
Known for
Crossover-Specific Holliday Junction Formation | Noncrossover Crossover | Highly Dynamic Process | Dna Strand Breaks
KOL-Index: 25 The Sgs1 helicase and Top3-Rmi1 decatenase form a complex that affects homologous recombination outcomes during the mitotic cell cycle and during meiosis. Previous studies have reported that Sgs1-Top3-Rmi1 function is regulated by SUMOylation that is catalyzed by the Smc5-Smc6-Mms21 complex. These studies used strains in which SGS1 was C-terminally tagged with three or six copies of a human ...
Known for
Helicase Homolog | Sufficient Sumoylation | Cerevisiae Bloom | Decatenase
KOL-Index: 22 We investigated the meiotic role of Srs2, a multi-functional DNA helicase/translocase that destabilises Rad51-DNA filaments and is thought to regulate strand invasion and prevent hyper-recombination during the mitotic cell cycle. We find that Srs2 activity is required for normal meiotic progression and spore viability. A significant fraction of srs2 mutant cells progress through both meiotic ...
Known for
Such Cells | Normal Recombination Intermediate Metabolism | Spo11-Induced Dsbs | Rpa
KOL-Index: 19 In Saccharomyces cerevisiae, the conserved Sgs1-Top3-Rmi1 helicase-decatenase regulates homologous recombination by limiting accumulation of recombination intermediates that are crossover precursors. In vitro studies have suggested that this may be due to dissolution of double-Holliday junction joint molecules by Sgs1-driven convergent junction migration and Top3-Rmi1 mediated strand ...
Known for
Restored Rmi1 | Depletion Delayed | Junction Joint | Molecule Resolution
KOL-Index: 19 The DNA double-strand breaks (DSBs) that initiate meiotic recombination in Saccharomyces cerevisiae are preceded first by DNA replication and then by a chromatin transition at DSB sites. This chromatin transition, detected as a quantitative increase in micrococcal nuclease (MNase) sensitivity, occurs specifically at DSB sites and not at other MNase-sensitive sites. Replication and DSB ...
Known for
Replication Timing | Sites Chromatin | Yeast Recombination | Chromosome Iii
KOL-Index: 19 Several protein kinases collaborate to orchestrate and integrate cellular and chromosomal events at the G2/M transition in both mitotic and meiotic cells. During the G2/M transition in meiosis, this includes the completion of crossover recombination, spindle formation, and synaptonemal complex (SC) breakdown. We identified Ipl1/Aurora B kinase as the main regulator of SC disassembly. Mutants ...
Known for
Crossovers | Binding Partner | Joint Molecule Resolution | Mitotic Meiotic Cells
KOL-Index: 19 The DNA double-strand breaks (DSBs) that form during meiosis I prophase initiate recombination. DSBs also play a critical role, in many species, in driving progressive association and colocalization of homologs, which culminate in full homolog synapsis at pachytene. Data from many species indicate that DSBs and recombination are not uniformly distributed, but occur more frequently in ...
KOL-Index: 18 In Saccharomyces cerevisiae , the meiosis-specific axis proteins Hop1 and Red1 are present nonuniformly across the genome. In a previous study, the meiosis-specific VMAl-derived endonuclease (VDE) was used to examine Spo11-independent recombination in a recombination reporter inserted in a Hop1/Red1-enriched region ( HIS4 ) and in a Hop1/Red1-poor region ( URA3 ). VDE-initiated crossovers at ...
Known for
Vde-Initiated Meiotic Recombination | Vde-Induced Crossovers | Mutlγ Meiotic | Ura3 Vde
KOL-Index: 17 Proteins with potential roles in meiotic recombination often have essential or important functions during the mitotic cell cycle. In addition, proteins may have different functions at different times during meiosis. In such cases, it can be challenging to precisely determine protein function during meiosis using null or hypomorphic mutants. One example is the Sgs1-Top3-Rmi1 ...
Known for
Mutants Sgs1 | Meiosis Proteins | Methods Protein | Isolation Meiotic

Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, 20892-4260, Bethesda, MD, USA NIH, Building 37, Room 6124, 37 Convent Dr. MSC4260, 20892-4260, Bethesda, MD, USA

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