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    • Analysis Of Mir-195 And...
    • Analysis of MiR-195 and MiR-497 Expression, Regulation and Role in Breast Cancer: Influence Statistics

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      Concepts for whichthey havehas direct influence:Mir195 mir497,Mir497 breast cancer,Breast cancer,Mir497 breast,Cancer cell,Mir497 expression,Targets mir195,Cpg islands.

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      #1
      Carlo Maria Croce
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      George Adrian Calin
      breast cancer gene expression chronic lymphocytic leukemia
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      Chang‐Gong Chang
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      Massimo Negrini
      hepatocellular carcinoma chronic lymphocytic leukemia gene expression
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      Hsiang‐fu Kung
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      Marilena Valeria Iorio
      breast cancer neoplastic humans gene expression

      Analysis of MiR-195 and MiR-497 Expression, Regulation and Role in Breast Cancer

      Abstract

      PURPOSE: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.

      EXPERIMENTAL DESIGN: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues.

      RESULTS: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer.

      CONCLUSIONS: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets.

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