• Mir195 Mir497
    • Analysis Of Mir-195 And...
    • Analysis of MiR-195 and MiR-497 Expression, Regulation and Role in Breast Cancer: Influence Statistics

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      Concepts for whichthey havehas direct influence:Mir195 mir497,Mir497 breast cancer,Breast cancer,Mir497 breast,Cancer cell,Mir497 expression,Targets mir195,Cpg islands.

      Key People For Mir195 Mir497

      Top KOLs in the world
      Carlo Maria Croce
      gene expression human chromosome tumor suppressor
      George Adrian Calin
      breast cancer gene expression chronic lymphocytic leukemia
      Chang‐Gong Chang
      gene expression gastric cancer irritable bowel syndrome
      Massimo Negrini
      hepatocellular carcinoma chronic lymphocytic leukemia gene expression
      Hsiang‐fu Kung
      hepatocellular carcinoma gastric cancer gene expression
      Marilena Valeria Iorio
      breast cancer neoplastic humans gene expression

      Analysis of MiR-195 and MiR-497 Expression, Regulation and Role in Breast Cancer


      PURPOSE: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.

      EXPERIMENTAL DESIGN: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes. Breast cancer cells stably expressing miR-195 and miR-497 were established to study their role and targets. Finally, normal, fibroadenoma and breast cancer tissues were employed to analyze the correlation between miR-195/497 levels and malignant stages of breast tumor tissues.

      RESULTS: MiR-195 and miR-497 were significantly downregulated in breast cancer. The methylation state of CpG islands upstream of the miR-195/497 gene was found to be responsible for the downregulation of both miRNAs. Forced expression of miR-195 or miR-497 suppressed breast cancer cell proliferation and invasion. Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497. miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer.

      CONCLUSIONS: Our data imply that both miR-195 and miR-497 play important inhibitory roles in breast cancer malignancy and may be the potential therapeutic and diagnostic targets.

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