• Disease
  • Epidermal
  • Epidermal Cells
  • Henry Hennings

    Prominent publications by Henry Hennings

    KOL Index score: 14580

    Mouse skin carcinomas arise from a small subpopulation of benign papillomas with an increased risk of malignant conversion. These papillomas arise with limited stimulation by tumor promoters, appear rapidly, and do not regress, suggesting that they differ in growth properties from the majority of benign tumors. The transforming growth factor beta (TGF-beta) proteins are expressed in the epidermis and are growth inhibitors for mouse keratinocytes in vitro; altered TGF-beta expression ...

    Also Ranks for: Malignant Conversion |  skin tumors |  factor beta |  transforming growth |  loss expression
    KOL Index score: 12103

    Transgenic mice that overexpress PKCalpha in the epidermis (K5-PKCalpha mice) exhibit acute CXCR2-mediated intraepidermal neutrophilic inflammation and a strong epidermal hyperplasia in response to application of 12-O-tetradecanoylphorbol-13-acetate (TPA). We now show that hyperplasia is independent of infiltrating neutrophils. Furthermore, when K5-PKCalpha mice were initiated with 7,12-dimethylbenz(a)anthracene (DMBA) and promoted with a low dose of TPA, 58% of K5-PKCalpha mice ...

    Also Ranks for: Skin Carcinogenesis |  cxcr2 ligands |  oncogenic ras |  cutaneous inflammation |  transformed keratinocytes
    KOL Index score: 11529

    The role of intracellular Ca2+ in the regulation of Ca(2+)-induced terminal differentiation of mouse keratinocytes was investigated using the intracellular Ca2+ chelator 1,2-bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA). A cell permeable acetoxymethyl (AM) ester derivative BAPTA (BAPTA/AM) was loaded into primary mouse keratinocytes in 0.05 mM Ca2+ medium, and then the cells were induced to differentiate by medium containing 0.12 or 0.5 mM Ca(2+) Intracellular BAPTA ...

    Also Ranks for: Intracellular Ca2 |  keratinocyte differentiation |  cell membrane |  increase cai |  egtazic acid
    KOL Index score: 11046

    Disaggregated epidermal cells, sheets of epidermis, and explants of partial and full-thickness skin have been grown in cell, organ, and explant cultures. Each type of epidermal sample has also been "cultured" as a graft on a living animal host. The extent of tissue-specific phenotypic expression by the epidermal cell varies with the type of culture and the culture conditions: medium, biologic and pharmacologic additives, substrate, cell density, pH, and temperature. Specific culture ...

    Also Ranks for: Epidermal Cells |  phenotypic expression |  cell culture |  basement membrane |  microscopy electron
    KOL Index score: 10720

    TUMOUR-PROMOTING agents have been defined by their ability to promote tumour formation on carcinogen-initiated mouse skin. The most potent of these agents are the diesters of phorbol which are the active components of croton oil, the classic promoting substance. Phorbol esters can be chemically modified in a number of positions to form molecules with a wide range of promoter potency1. Such modifications have been useful in determining structure-function relationships2. A good correlation ...

    Also Ranks for: Phorbol Esters |  dna synthesis |  decarboxylase activity |  mouse epidermal |  cell cultures
    KOL Index score: 10232

    Calcium modulation of keratinocyte growth in culture was studied by both transmission (TEM) and scanning electron microscopy (SEM). Under standard culture conditions (1.2-1.8 mM calcium), cells were connected by desmosomes and stratified to 4-6 cell layers. Many aspects of in vitro epidermal maturation were analogous to the in vivo process, with formation of keratohyalin granules, loss of nuclei, formation of cornified envelopes and shedding of cornified cells containing keratin ...

    Also Ranks for: Epidermal Cells |  electron microscopy |  calcium regulation |  ultrastructural study |  keratin filaments
    KOL Index score: 9894

    Modification of the ionic calcium concentration in the culture medium markedly alters the pattern of proliferation and differentiation in cultured mouse epidermal cells. When medium calcium is lowered to 0.05--0.1 mM, keratinocytes proliferate rapidly with a high growth fraction and do not stratify, but continue to synthesize keratin. The cells grow as a monolayer for several months and can be subcultured and cloned in low Ca++ medium. Ultrastructural examination of cells cultured under ...

    Also Ranks for: Epidermal Cells |  calcium regulation |  culture medium |  mice mice |  protein synthesis


    Henry Hennings: Influence Statistics

    Sample of concepts for which Henry Hennings is among the top experts in the world.
    Concept World rank
    croton oil extent #1
    mitotic rate inhibition #1
    initial 40–50 inhibition #1
    evaluation retinoids #1
    dimethylbenzaanthracene binding #1
    neoplasms urethane animals #1
    retinoids tumor promoters #1
    injections growth parameters #1
    papillomas rate #1
    repeated injections chalone #1
    single mutational event7 #1
    tpa 20 weeks #1
    acid 20 weeks #1
    extracts inhibiting activity #1
    promoted papillomas #1
    combined effects hydrocortisone #1
    promoted mezerein #1
    croton oilinduced increase #1
    injection water extracts #1
    cellophane dna #1
    agent hydrocortisone #1
    tumors initiated #1
    maximum reduction 12 #1
    actinomycin skin #1
    chalone repeated injections #1
    regression tumour size #1
    initiation accomplished #1
    individual tpa applications #1
    urethane 910 #1
    51 micrograms acid #1
    mezerein promoted #1
    papillomas tpa #1
    kinetics precede #1
    9–12 hours #1
    promoted tpa #1
    initiated mezerein #1
    decrease mitotic rate #1
    initiation single exposure #1
    5 thymidine3h incorporation #1
    cell flux g1 #1
    intraperitoneal liver skin #1
    aqueous extract liver #1
    intraperitoneal injection injections #1
    mitotic rate 8–10 #1
    papillomas develop #1
    epidermal chalone depression #1
    mnng initiated #1

    Key People For Epidermal Cells

    Top KOLs in the world
    Howard A Green
    adipose conversion involucrin gene 3t3 cells
    Elaine V Fuchs
    stem cells intermediate filaments transgenic mice
    Stuart H Yuspa
    epidermal cells protein kinase malignant conversion
    Stephen I Katz
    langerhans cells basement membrane dermatitis herpetiformis
    Fiona M Watt
    stem cells terminal differentiation proton beam
    Georg Stingl
    langerhans cells atopic dermatitis inbred balb mice

    Henry Hennings:Expert Impact

    Concepts for whichHenry Henningshas direct influence:Epidermal cells,  Malignant conversion,  Mouse skin,  Dna synthesis,  Inbred balb mice,  Intracellular ca2,  Tumor promotion,  Terminal differentiation.

    Henry Hennings:KOL impact

    Concepts related to the work of other authors for whichfor which Henry Hennings has influence:Protein kinase,  Human keratinocytes,  Epidermal cells,  Mouse skin,  Keratinocyte differentiation,  Growth factor,  Cell proliferation.



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    Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH, Bethesda, Maryland | Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH, Bethesda, MD, USA | Laboratory of Cellular Carcinogenesis and Tumor Promotion,