Prominent publications by Arne Tärnvik

KOL Index score: 13527

Intracellular killing of Francisella tularensis by macrophages depends on interferon-gamma (IFN-gamma)-induced activation of the cells. The importance of inducible nitric oxide synthase (iNOS) or NADPH phagocyte oxidase (phox) for the cidal activity was studied. Murine IFN-gamma-activated peritoneal exudate cells (PEC) produced nitric oxide (NO), measured as nitrite plus nitrate, and superoxide. When PEC were infected with the live vaccine strain, LVS, of F. tularensis, the number of ...

Known for Tularensis Lvs |  Oxygen Species |  Reactive Nitrogen |  Murine Macrophages |  Nitric Oxide
KOL Index score: 12375

Reactive nitrogen species (RNS) and reactive oxygen species (ROS) are important mediators of the bactericidal host response. We investigated the contribution of these two mediators to the control of infection with the facultative intracellular bacterium Francisella tularensis. When intradermally infected with the live vaccine strain F. tularensis LVS, mice deficient in production of RNS (iNOS(-/-) mice) or in production of ROS by the phagocyte oxidase (p47(phox-/-) mice) showed ...

Known for Oxygen Species |  Reactive Nitrogen |  Control Infection |  Rns Ros |  Distinct Roles
KOL Index score: 12368

The live vaccine strain (LVS) of Francisella tularensis is killed by human polymorphonuclear leukocytes as a result of strictly oxygen-dependent mechanisms (S. Löfgren, A. Tärnvik, M. Thore, and J. Carlsson, Infect. Immun. 43:730-734, 1984). We now report that a capsule-deficient (Cap-) mutant of LVS survives in the leukocytes. In contrast to the encapsulated parent strain, the Cap- mutant was avirulent in mice and was susceptible to the bactericidal effect of nonimmune human serum. The ...

Known for Tularensis Lvs |  Polymorphonuclear Leukocytes |  Parent Strain |  Deficient Mutant |  Bactericidal Serum
KOL Index score: 11603

A hantavirus infection is followed by a prominent antibody response to the viral nucleocapsid protein. Antibodies from patients infected with one hantavirus cross-react to varying degrees with the nucleocapsid protein of other viruses of the genus. We studied the cross-reactivity in serially obtained blood samples from 17 patients with nephropathia epidemica, a European form of hemorrhagic fever with renal syndrome caused by Puumala virus. Recombinant truncated nucleocapsid protein (aa ...

Known for Nucleocapsid Protein |  Nephropathia Epidemica |  Puumala Virus |  Viral Cross |  Antibodies Patients
KOL Index score: 11238

Microbial ligands, including lipopolysaccharide (LPS) and bacterial lipoproteins, activate Toll-like receptors (TLR) of mononuclear phagocytes, thereby inducing proinflammatory cytokines and antimicrobial activity. We show that Francisella tularensis, an intracellular pathogen, is capable of inhibiting this macrophage response. Infection with the live vaccine strain F. tularensis LVS rendered cells of the murine macrophage-like cell line J774A.1 incapable of secreting TNF-alpha or ...

Known for Francisella Tularensis |  Antimicrobial Activity |  J774 Cells |  Murine Macrophages |  Lvs Lps
KOL Index score: 9539

Plasma levels of cytokines were measured by EIA in 15 subjects hospitalized with nephropathia epidemica, a European form of hantavirus-induced hemorrhagic fever with renal syndrome. Concentrations of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were increased in all patients at admission, and the concentration of IL-10 was increased in most. TNF-alpha concentrations were still increased 1 week after onset of disease; levels of IL-6 and IL-10 were normalized. TNF-alpha was ...

Known for Tumor Necrosis |  Hemorrhagic Fever |  Renal Syndrome |  Plasma Levels |  Nephropathia Epidemica
KOL Index score: 9142

A 17-kDa lipoprotein, TUL4, of the facultative intracellular bacterium Francisella tularensis is one of several membrane proteins that induce an in vitro response in T cells from F. tularensis-primed humans. A DNA fragment of the live vaccine strain F. tularensis LVS encoding TUL4 was cloned into Salmonella typhimurium chi 4072, an attenuated delta cya delta crp mutant. Expression of the protein by the recombinant S. typhimurium chi 4072 (pTUL4-15) was maintained after passage in BALB/cJ ...

Known for Salmonella Typhimurium |  Francisella Tularensis |  Live Vaccine |  Antibody Response |  Immunized Mice
KOL Index score: 8949

PCR and culture were comparatively evaluated for their abilities to demonstrate Francisella tularensis in wound specimens from tularemia patients during an outbreak in Sweden in 1998. For transport of the specimens used for PCR, a buffer solution containing a nuclease inhibitor was used, and for transport of the specimens used for culture, a commercial transport system was selected after experimental comparison of various systems. Of 40 patients with culture- and/or serology-verified ...

Known for Pcr Culture |  Tularemia Patients |  Wound Specimens |  Tularensis Dna |  Specimen Handling
KOL Index score: 8760

BACKGROUND: In current renewal of medical education, problem-based learning (PBL) is the predominant approach. PBL is afflicted with limitations, which cause uncertainness about its future. A profoundly different approach is the case method, developed a century ago and today attracting much less interest in developmental work than PBL.

AIM: To compare the characteristics of PBL and the case method and ask the question of whether the case method may serve as an alternative approach to ...

Known for Case Method |  Learning Pbl |  Medical Education |  Problem Based |  Student Centred
KOL Index score: 8714

T cells are crucial to the control and eradication of the facultative intracellular bacterium Francisella tularensis. A contributory role of humoral antibodies in the host defence remains to be assessed. We used B-cell-deficient mice to study the possible contribution of antibodies to the defence against the live vaccine strain (LVS) or a clinical isolate of F. tularensis, both belonging to the subspecies holarctica (type B). When B-cell-deficient (Igmu(-/-)) mice of the C57BL/10 ...

Known for Tularensis Subsp |  Specific Antibodies |  Immune Serum |  Liver Spleen |  Live Vaccine Strain
KOL Index score: 8571

The adaptation of facultative intracellular bacteria to host macrophages involves regulation of the synthesis of bacterial proteins. We analyzed the protein synthesis of Francisella tularensis LVS growing intracellularly in the macrophage-like murine cell line J774 and extracellularly in culture medium. After pulse-labeling with [35S] methionine and separation by one- and two-dimensional polyacrylamide gel electrophoresis, induction of a few proteins during intracellular growth was ...

Known for Francisella Tularensis |  23kda Protein |  Intracellular Growth |  Hydrogen Peroxide |  Data Molecular
KOL Index score: 8321

The response of the facultative intracellular bacterium Francisella tularensis LVS to stress was assayed by pulse-labeling with [35S]methionine followed by two-dimensional gel electrophoresis and autoradiography. A temperature increase from 37 to 42 degrees C or exposure to 5 mM hydrogen peroxide induced increased syntheses of at least 15 proteins. Among these proteins were a 75-, a 60-, and a 10-kDa protein. By N-terminal sequence analysis, these three proteins were found to be ...

Known for Hydrogen Peroxide |  Tularensis Lvs |  Proteins Heat |  Chaperonin 60 |  Intracellular Survival
KOL Index score: 8147

Immunostimulating complexes (ISCOMs) are known to be highly effective adjuvants for envelope antigens of viral agents, but have not been evaluated for use with antigens of intracellular bacteria. Balb/c mice were subcutaneously immunized with ISCOMs into which the T cell-reactive membrane protein TUL4 of Francisella tularensis had been incorporated. Spleen cells from the immunized mice responded in vitro to TUL4 and to heat-killed F. tularensis live vaccine strain (LVS) with ...

Known for Francisella Tularensis |  Immunized Mice |  Immunity Cellular |  Immunologic Animals Antibodies |  Bacterial Proteins
KOL Index score: 7669

The groE operon of Francisella tularensis LVS, encoding the heat shock proteins chaperone-10 (Cpn10) and Cpn60, was sequenced and characterized, and the T-cell response of LVS-vaccinated individuals to the two proteins and the third major chaperone, Ft-DnaK, was assayed. The cpn10 and cpn60 genes were amplified by PCR with degenerate oligonucleotides derived from the N-terminal sequence of the two proteins. The sequence analysis revealed the expected two open reading frames, encoding ...

Known for Francisella Tularensis |  Shock Proteins |  Nucleotide Sequence |  Gel Dimensional |  Reading Frames


Arne Tärnvik: Influence Statistics

Sample of concepts for which Arne Tärnvik is among the top experts in the world.
Concept World rank
normal population1 association #1
lymphocyte response onset #1
o2 phagocytosed pneumococci #1
nearby rural parts #1
postpbl #1
lymphocytes onset #1
viable attenuated strain #1
acute leukaemia2 #1
thymidine tritium cells #1
phytohaemagglutinin fraction #1
low extent months #1
anaerobiosis killing #1
bacteria francisella #1
layer dna depression #1
phacoated cell membranes #1
17 kilodalton #1
fractions agglutinated cells #1
lymphocytes vaccinated subjects #1
wild living animals #1
pha cell membrane #1
cell membranes phytohaemagglutinin #1
retain student #1
treatment tularemia #1
bacteria attenuated strain #1
hphapinduced agglutination #1
degradation teichoic #1
lymphocyte stimulation complex #1
strain francisella #1
lphapinduced stimulation glycoprotein #1
r−064 p002 #1
leucocytesplatelets cells #1
phytohaemagglutinin lymphocyte #1
aerobic leukocytes #1
lymphocytes individuals #1
case method #1
glycoprotein cell membranes #1
teacher process #1
monocytes lymphocyte preparation #1
responded membranes #1
degradation leukocytes #1

Key People For Francisella Tularensis

Top KOLs in the world
Anders B Sjöstedt
francisella tularensis schu s4 immune mice
Richard W Titball
yersinia pestis clostridium perfringens francisella tularensis
Petra C F Oyston
yersinia pestis francisella tularensis essential genes
Arne Tärnvik
francisella tularensis nephropathia epidemica puumala virus
francisella tularensis schu s4 genome sequence
Igor R Golovliov
francisella tularensis schu s4 nitric oxide

Arne Tärnvik:Expert Impact

Concepts for whichArne Tärnvikhas direct influence:Francisella tularensis,  Nephropathia epidemica,  Vaccine strain,  Case method,  Tularensis lvs,  Nucleocapsid protein,  Puumala virus,  Hemorrhagic fever.

Arne Tärnvik:KOL impact

Concepts related to the work of other authors for whichfor which Arne Tärnvik has influence:Francisella tularensis,  Renal syndrome,  Porphyromonas gingivalis,  Hemorrhagic fever,  Hantavirus infection,  Live vaccine strain,  Schu s4.



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Department of Clinical Microbiology, Umeå University, Umeå, Sweden | Department of Clinical Microbiology, Umeå University, | Department of Infectious Diseases, Umeå University, 901 85 Umeå, Sweden | Infe

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