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  • Neutral Protease
  • Daniel J Mccarty

    Prominent publications by Daniel J Mccarty

    KOL Index score: 10568

    OBJECTIVE: To analyze ANKH in families with calcium pyrophosphate dihydrate crystal deposition disease (CPPD) for disease-causing mutations.

    METHODS: Two US families (one of British ancestry and the other of German/Swiss ancestry) with autosomal-dominant CPPD, whose disease phenotypes were found to be linked to chromosome 5p15.1 (locus symbol CCAL2), were screened by direct sequencing for mutations in ANKH, a gene in the CCAL2 candidate interval that has been shown to harbor mutations in ...

    Also Ranks for: Mutations Ankh |  crystal deposition |  pyrophosphate dihydrate |  amino terminus |  direct sequencing
    KOL Index score: 10187

    Hydroxyapatite crystals in spheroid-shaped masses 1.9-15.6 micrometer in diameter were found in 12 of 13 synovial fluids obtained from the shoulder joints of 4 patients with rotator cuff tears and glenohumeral osteoarthritis. Two of 16 control joint fluids also showed these particles. Collagen types I, II and III were identified in the joint fluid pellets from 3 of the 4 patients, and fibers with typical collagen periodicity were also seen on transmission electronmicroscopy. Collagenase ...

    Also Ranks for: Neutral Protease |  active collagenase |  synovial fluid |  hydroxyapatite crystals |  rotator cuff defects
    KOL Index score: 8082

    A rapid and relatively simple method for measurement of inorganic pyrophosphate (PPi) in biological samples has been described. The mean +/-SEM of plasma samples from 94 normal subjects was 1.8+/-0.06 muM, giving a normal range (99% confidence limits) of 0.16 - 3.40 mumol/liter. Analysis of 17 plasma samples in duplicate showed a standard deviation of 0.18, giving a 99% probability that a single determination of plasma PPi would be +/-0.68 muM of the true value. The mean PPi levels in ...

    Also Ranks for: Biological Fluids |  inorganic pyrophosphate |  ppi levels |  plasma samples |  aged osteoarthritis
    KOL Index score: 7647

    Fifty-five samples from knee joints of 44 patients with a wide variety of joint diseases were analyzed by routine gross and microscopic examination, and for pH, pO2 and pCO2; 18 fluids were analyzed for lactate. pO2 values ranged from 8–78 mm Hg. Lower values were found generally in fluids of the inflammatory type. pH values ranged between 6.6 and 7.41 units and correlated poorly with pO2. pCO2 ranged between 34 and 150 mm Hg. pO2 correlated poorly with pCO2 (r = −0.22) but better with ...

    Also Ranks for: Joint Diseases |  reactive arthritis |  po2 pco2 |  partial pressure |  carbon dioxide
    KOL Index score: 7388

    Hydroxyapatite (HA) and calcium pyrophosphate dihydrate (CPPD) crystals were phagocytosed when added to cultured human rheumatoid or normal canine synovial cells. Collagenase and neutral protease secretion into the culture medium was increased 5- to 8-fold over control values in the presence of HA and increased 3-fold in the presence of CPPD crystals. HA but not CPPD crystals induced a 300-fold increase in human rheumatoid synovial cell culture fluid prostaglandin (PG) E2 levels and an ...

    Also Ranks for: Neutral Protease |  calcium pyrophosphate |  synovial cells |  cppd crystals |  culture medium
    KOL Index score: 6935

    Synovial tissue excised from the unstable right shoulder joint of a patient with an absent rotator cuff, severe glenohumeral joint degeneration, and hydroxyapatite-containing microspherules, collagen types I, II, and III, active collagenase, and neutral protease in the joint fluid showed extensive osteochondromatosis histologically. Electronmicroscopy revealed calcific foci in microvilli which could easily escape into the adjacent joint space through areas denuded of synovial cells. ...

    Also Ranks for: Neutral Protease |  active collagenase |  rotator cuff defects |  “milwaukee shoulder”—association |  joint fluid
    KOL Index score: 6826

    Two rheumatic syndromes associated with deposition of calcium phosphate crystals in soft tissues of the shoulder have prompted us to study the cellular mechanisms of calcium phosphate crystal solubilization. Synthetic45Ca labeled calcium phosphate crystal aggregates were solubilized by cultured human fibroblasts or monocytes. Such solubilization required crystal cell contact and was inhibited by chloroquine and ammonium. We hypothesize that the mechanism of crystal solubilization ...

    Also Ranks for: Calcium Phosphate |  cultured human |  synovial cells |  phagocytosis dissolution |  acidic environment
    KOL Index score: 6717

    Serum inorganic pyrophosphate (PPi) levels were consistently two- to threefold higher than plasma PPi prepared from the same blood. PPi was found in platelets in amounts ranging from 1.4 to 3 nmol/10(8) cells, using three different techniques for quantification. These levels are approximately 800 times higher than the mean PPi concentration in normal plasma and approximate the levels of ADP found in platelets by other workers. About 50% of platelet PPi was specifically released ...

    Also Ranks for: Inorganic Pyrophosphate |  human platelets |  plasma ppi |  extracellular medium |  platelet calcium
    KOL Index score: 6624

    Four women, aged 63 to 90 years old, presented with mildly painful shoulders of decreased mobility or stability. Radiographic evidence of a complete tear of the fibrous rotator cuff was present in 7 of 8 shoulder joints. Microspheroids containing hydroxyapatite crystals were seen by scanning electronmicroscopy in 12 of 13 synovial fluid samples. All synovial fluids showed activated collagenase and neutral protease activity. This constellation of findings represents a heretofore ...

    Also Ranks for: Neutral Protease |  milwaukee shoulder |  active collagenase |  clinical aspects |  rotator cuff defects
    KOL Index score: 6558

    Murine peritoneal macrophages were incubated with45Ca-labeled basic calcium phosphate (BCP) crystals in the presence or absence of cytochalasin B. Untreated macrophages solubilized 30–50% of45Ca-BCP in 24 hours. Dissolution began within 3 hours and was linear thereafter. Twenty-three percent of BCP was cell-associated by 3 hours. Endocytosis of crystal occurred continuously throughout the incubation. Endocytosis of crystal did not affect the migration of macrophages through Percoll ...

    Also Ranks for: Basic Calcium |  phosphate crystals |  endocytosis dissolution |  24 hours |  macrophages mice
    KOL Index score: 5748

    Synovial fluid (SF) inorganic pyrophosphate (PPi) concentration is elevated in calcium pyrophosphate dihydrate (CPPD) crystal deposition arthropathy. Since CPPD and basic calcium phosphate (BCP) crystals often are present in the same joints, we determined [PPi] and activity of the PPi-generating enzyme, nucleotide pyrophosphohydrolase (NPPH), in SF from the joints of patients with various arthropathies, including those with BCP crystals. We found elevated SF [PPi] in joints with BCP ...

    Also Ranks for: Basic Calcium |  synovial fluid |  bcp crystals |  inorganic pyrophosphate |  shoulder syndrome


    Daniel J Mccarty: Influence Statistics

    Sample of concepts for which Daniel J Mccarty is among the top experts in the world.
    Concept World rank
    mitogenosis #1
    milwaukee shoulder #1
    28 pseudogout patients #1
    gout inconstant feature #1
    mitogenic crystals #1
    cppd 4 subjects #1
    crystal shedding #1
    day disease suppression #1
    endothelium palindromic rheumatism #1
    arthritis ppi levels #1
    14cadeninelabeled nucleotides #1
    crystal equivalent effects #1
    ecto ntppph #1
    crystals rabbit joints #1
    intimate crystalcell contact #1
    devolutionary joint #1
    neutral protease patients #1
    recurrent acute inflammation #1
    neutral protease prostaglandins #1
    hemochromatosis urate #1
    uronic acid production #1
    gout hemochromatosis #1
    equal proportions type #1
    3 hours endocytosis #1
    differential diphosphates humans #1
    frostbite child #1
    intracellular ppi cppds #1
    freezing microvascular damage #1
    authors introductory overview #1
    understanding resorption #1
    cppds control fibroblasts #1
    crystals soft tissues #1
    articular tenderness humans #1
    hydroxyapatite crystals electronmicroscopy #1
    cppd crystal solubility #1
    genesis articular lesions #1
    lipoproteins male microscopy #1
    control joints values #1
    acute pseudogout hypothesis #1
    endocytosis mitogenic #1

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    Daniel J Mccarty:Expert Impact

    Concepts for whichDaniel J Mccartyhas direct influence:Neutral protease,  Synovial fluid,  Rheumatoid arthritis,  Active collagenase,  Calcium pyrophosphate,  Hydroxyapatite crystals,  Milwaukee shoulder syndrome,  Inorganic pyrophosphate.

    Daniel J Mccarty:KOL impact

    Concepts related to the work of other authors for whichfor which Daniel J Mccarty has influence:Rheumatoid arthritis,  Synovial fluid,  Gout patients,  Uric acid,  Reflex sympathetic dystrophy,  Calcium pyrophosphate,  Articular cartilage.



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