Prominent publications by Vanaporn Wuthiekanun

KOL Index score: 12935

BACKGROUND: Rapid PCR-based tests for the diagnosis of leptospirosis can provide information that contributes towards early patient management, but these have not been adopted in Thailand. Here, we compare the diagnostic sensitivity and specificity of two real-time PCR assays targeting rrs or lipL32 for the diagnosis of leptospirosis in northeast Thailand.

METHODS/PRINCIPAL FINDINGS: A case-control study of 266 patients (133 cases of leptospirosis and 133 controls) was constructed to ...

Known for Human Leptospirosis |  Ribosomal Rna |  Pcr Assays |  Rrs Assay |  Diagnostic Sensitivity
KOL Index score: 12860

BACKGROUND: A sustained outbreak of leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown.

METHODS AND FINDINGS: A prospective study was conducted between 2000 and 2005 to identify patients with leptospirosis presenting to Udon Thani Hospital in northeast Thailand, and to isolate the causative organisms from blood. A multilocus sequence typing scheme was developed to genotype these pathogenic Leptospira. Additional typing was performed for ...

Known for Leptospira Interrogans |  Human Leptospirosis |  Bacterial Rna |  Northeast Thailand |  Sequence Typing
KOL Index score: 12711

Infections with Salmonella enterica serovar Typhi isolates that are multidrug resistant (MDR: resistant to chloramphenicol, ampicillin, trimethoprim-sulphamethoxazole) with intermediate ciprofloxacin susceptibility are widespread in Asia but there is little information from Cambodia. We studied invasive salmonellosis in children at a paediatric hospital in Siem Reap, Cambodia. Between 2007 and 2011 Salmonella was isolated from a blood culture in 162 children. There were 151 children with ...

Known for Enteric Fever |  Cambodian Children |  Serovar Typhi |  Intermediate Susceptibility |  Siem Reap
KOL Index score: 12603

Staphylococcus argenteus is a newly named species previously described as a divergent lineage of Staphylococcus aureus that has recently been shown to have a global distribution. Despite growing evidence of the clinical importance of this species, knowledge about its population epidemiology and genomic architecture is limited. We used whole-genome sequencing to evaluate and compare S. aureus (n = 251) and S. argenteus (n = 68) isolates from adults with staphylococcal sepsis at several ...

Known for Staphylococcus Argenteus |  Northeastern Thailand |  St2250 Isolates |  Species Aureus |  Genome Bacterial
KOL Index score: 12490

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the ...

Known for Burkholderia Species |  Assisted Laser |  Identification Pseudomallei |  Mass Spectrometry |  Ionization Time
KOL Index score: 11956

BACKGROUND: Culture remains the diagnostic gold standard for many bacterial infections, and the method against which other tests are often evaluated. Specificity of culture is 100% if the pathogenic organism is not found in healthy subjects, but the sensitivity of culture is more difficult to determine and may be low. Here, we apply Bayesian latent class models (LCMs) to data from patients with a single Gram-negative bacterial infection and define the true sensitivity of culture together ...

Known for Serological Tests |  Culture Gold Standard |  Diagnosis Melioidosis |  Latent Class |  Sensitivities Specificities
KOL Index score: 11797

Samples from 160 prospectively recruited febrile patients with typhus-like illness in an area of Thailand (Chiang Rai, northern Thailand) where scrub typhus is endemic were used to evaluate the diagnostic capabilities of four rapid immunochromatographic tests (ICTs) for the detection of Orientia tsutsugamushi IgM and total antibodies during acute scrub typhus infection. Of the 160 cases, 54 (34%) had been confirmed to have scrub typhus using the reference scrub typhus infection criteria ...

Known for Scrub Typhus |  Orientia Tsutsugamushi |  95 Specificity |  Isothermal Amplification |  Bacterial Humans
KOL Index score: 11729

Melioidosis is a severe infection caused by Burkholderia pseudomallei. The timely implementation of effective antimicrobial treatment requires rapid diagnosis. Loop-mediated isothermal amplification (LAMP) targeting the TTS1 gene cluster was developed for the detection of B. pseudomallei. LAMP was sensitive and specific for the laboratory detection of this organism. The lower limit of detection was 38 genomic copies per reaction, and LAMP was positive for 10 clinical B. pseudomallei ...

Known for Burkholderia Pseudomallei |  Pcr Lamp |  Isothermal Amplification |  Sensitivity Specificity |  Lower Limit
KOL Index score: 11413

Human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. Here, we define the rate of relapse versus the rate of reinfection in 116 patients with 123 episodes of recurrent melioidosis who were treated at Sappasithiprasong Hospital in Northeast Thailand between 1986 and 2005. Pulsed-field gel electrophoresis was performed on all isolates; isolates from primary and recurrent disease for a given patient different by one or more bands ...

Known for Northeast Thailand |  Recurrent Melioidosis |  Relapse Reinfection |  Sappasithiprasong Hospital |  Electrophoresis Gel
KOL Index score: 11049

Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated whole-cell lysates of all Leptospira spp., while MAb LD5 and MAb LE1, which were IgG1 and IgG2a, respectively, reacted to the 35- to ...

Known for Human Leptospirosis |  Bacterial Antibodies |  Monoclonal Antigens |  Urine Samples |  Leptospira Antigen
KOL Index score: 11047

The soil bacterium and potential biothreat agent Burkholderia pseudomallei causes the infectious disease melioidosis, which is naturally acquired through environmental contact with the bacterium. Environmental detection of B. pseudomallei represents the basis for the development of a geographical risk map for humans and livestock. The aim of the present study was to develop a highly sensitive, culture-independent, DNA-based method that allows direct quantification of B. pseudomallei from ...

Known for Soil Samples |  Time Pcr |  Pseudomallei Dna |  Direct Detection |  Culture Positive
KOL Index score: 11037

BACKGROUND: Febrile illnesses are pre-eminent contributors to morbidity and mortality among children in South-East Asia but the causes are poorly understood. We determined the causes of fever in children hospitalised in Siem Reap province, Cambodia.

METHODS AND FINDINGS: A one-year prospective study of febrile children admitted to Angkor Hospital for Children, Siem Reap. Demographic, clinical, laboratory and outcome data were comprehensively analysed. Between October 12(th) 2009 and ...

Known for Febrile Illness |  Southeast Asia |  Siem Reap |  Children Cambodia |  Dengue Virus
KOL Index score: 10969

BACKGROUND: We observed that some patients with clinical leptospirosis supported by positive results of rapid tests were negative for leptospirosis on the basis of our diagnostic gold standard, which involves isolation of Leptospira species from blood culture and/or a positive result of a microscopic agglutination test (MAT). We hypothesized that our reference standard was imperfect and used statistical modeling to investigate this hypothesis.

METHODS: Data for 1652 patients with ...

Known for Reference Tests |  Mat Culture |  95 Cri |  Lateral Flow |  Gold Standard
KOL Index score: 10238

BACKGROUND: Melioidosis is a frequently fatal infectious disease caused by the soil dwelling Gram-negative bacterium Burkholderia pseudomallei. Environmental sampling is important to identify geographical distribution of the organism and related risk of infection to humans and livestock. The aim of this study was to evaluate spatial distribution of B. pseudomallei in soil and consider the implications of this for soil sampling strategies.

METHODS AND FINDINGS: A fixed-interval sampling ...

Known for Pseudomallei Soil |  Rice Field |  Northeast Thailand |  Environmental Sampling |  Spatial Distribution
KOL Index score: 10178

BACKGROUND: The indirect immunofluorescence assay (IFA) is considered a reference test for scrub typhus. Recently, the Scrub Typhus Infection Criteria (STIC; a combination of culture, PCR assays and IFA IgM) were proposed as a reference standard for evaluating alternative diagnostic tests. Here, we use Bayesian latent class models (LCMs) to estimate the true accuracy of each diagnostic test, and of STIC, for diagnosing scrub typhus.

METHODS/PRINCIPAL FINDINGS: Data from 161 patients with ...

Known for Diagnostic Tests |  Scrub Typhus |  Bayesian Lcms |  Reference Test |  Ifa Igm


Vanaporn Wuthiekanun: Influence Statistics

Sample of concepts for which Vanaporn Wuthiekanun is among the top experts in the world.
Concept World rank
ashdowns #1
rate recurrent disease #1
ceftazidime disease models #1
casecontrol study hospitals #1
melioidosis deaths #1
vanaporn wuthiekanun #1
clinical burkholderia #1
clinical isolates thailand #1
virulence thailandensis #1
pseudomallei positive #1
melioidosis microscopy #1
lipopolysaccharides melioidosis #1
clinical awareness melioidosis #1
pseudomallei thailandensis #1
pseudomallei biotypes #1
wuthiekanun lvw #1
quantitative pseudomallei #1
pseudomallei culture #1
patients multiyear outbreak #1
pseudomallei lao #1
avirulent burkholderia #1
pseudomallei soil samples #1
ashdowns medium #1
melioidosis bacteremic melioidosis #1
latex fixation specificity #1
191 size #1
true burden disease #1
melioidosis central thailand #1
northeast thailand relapse #1
pseudomallei soil #1
sputum suppuration #1
soil lao #1
prevalence studies thai #1
aim vitro interaction #1
iqr relapse #1
concentrations insulin preparation #1
melioidosis thai #1
border melioidosis #1
patients suspected melioidosis #1
melioidosis bangkok #1
leptospira vanaporn #1
flagellin gene fragments #1
stable marker #1
samples pseudomallei #1
humans melioidosis sensitivity #1
pseudomallei antigen #1
burkholderia absence #1
pseudomallei vitek #1
vanaporn #1
rice farmer #1

Key People For Burkholderia Pseudomallei

Top KOLs in the world
Bart J Currie
northern territory burkholderia pseudomallei crusted scabies
Nicholas John White
falciparum malaria plasmodium vivax southeast asia
Sharon J Peacock
staphylococcus aureus burkholderia pseudomallei northeast thailand
Vanaporn Wuthiekanun
burkholderia pseudomallei northeast thailand leptospira spp
Allen C Cheng
new zealand northern territory staphylococcus aureus
Wipada Chaowagul
northeast thailand pseudomonas pseudomallei severe melioidosis

Vanaporn Wuthiekanun:Expert Impact

Concepts for whichVanaporn Wuthiekanunhas direct influence:Burkholderia pseudomallei,  Northeast thailand,  Pseudomonas pseudomallei,  Recurrent melioidosis,  Soil samples,  Leptospira spp,  Southeast asia,  Orientia tsutsugamushi.

Vanaporn Wuthiekanun:KOL impact

Concepts related to the work of other authors for whichfor which Vanaporn Wuthiekanun has influence:Burkholderia pseudomallei,  Scrub typhus,  Staphylococcus aureus,  Southeast asia,  Antimicrobial resistance,  Orientia tsutsugamushi,  Leptospira spp.



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Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. | Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, 10400, Thailand | Mahidol Oxford Tropical Medicine Res

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