• Polyclonal Antibodies
    • Enzyme-Linked...
    • Enzyme-Linked Immunosorbent Assay-Based Detection of Free Trenbolone in Bovine Bile: Influence Statistics

      Expert Impact

      Concepts for whichthey havehas direct influence:Polyclonal antibodies,Trenbolone acetate,Immunosorbent assay,Linked immunosorbent,Enzyme linked,Anabolic agents,Antibody based.

      Key People For Polyclonal Antibodies

      Top KOLs in the world
      bacteriophage t4 structural proteins disc genes genetics
      Julian Gordon
      ribosomal proteins elongation factor s6 phosphorylation
      Harry Towbin
      monoclonal antibodies mx protein blood mononuclear cells
      Theophil Staehelin
      initiation factors messenger rna monoclonal antibodies
      Marion M Bradford
      corona radiata modulator protein mammalian fertilization
      quantitative histochemistry glucuronidase hydrolysis brain iii

      Enzyme-Linked Immunosorbent Assay-Based Detection of Free Trenbolone in Bovine Bile


      A rapid antibody-based detection system has been developed for the presence of free trenbolone in bovine samples. Polyclonal antibodies were produced that showed specificity toward epitopes located around the steroidal A-ring of the trenbolone molecule. These antibodies were shown to have little or no recognition for many closely related compounds. The antibodies were utilized as the specific biorecognition molecules in competitive and inhibitive enzyme-linked immunosorbent assay systems. While both assays were able to detect low nanogram concentrations of trenbolone in bovine bile, the competitive format was more sensitive (2.41 vs 17.15 ng/mL for TRAb2 and 3.31 vs 30.73 ng/mL for TRAb1). This format was also more accurate and the data produced by this assay fitted more closely to the four parameter equation used to calculate the standard curve. This was a common finding with both of the polyclonal antibodies, suggesting that this was a characteristic of the format used.

      Sign-in to see all concepts, it's free!


    Download on the App StoreGet it on Google Play

    Copyright © 2023 Key Opinion Leaders, LLC.