• Disease
  • Francisella
  • Francisella Tularensis
  • Anders B Sjöstedt

    Prominent publications by Anders B Sjöstedt

    KOL Index score: 16536

    The two subspecies of Francisella tularensis, F. tularensis subsp. tularensis (type A) and F. tularensis subsp. palaearctica (type B), differ from each other in biochemistry and virulence. Strains of F. tularensis subsp. tularensis are believed to be confined to North America, whereas strains of F. tularensis subsp. palaearctica occur in Europe, in Asia, and in North America. Moreover, the existence of two other subspecies, designated F. tularensis subsp. mediaasiatica and F. tularensis ...

    Also Ranks for: Tularensis Subsp |  north america |  palaearctica japonica |  strains francisella |  soviet union
    KOL Index score: 16453

    Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and F. tularensis subsp. novicida. We developed a DNA microarray based on 1,832 clones from a shotgun library used for sequencing of the highly ...

    Also Ranks for: Tularensis Subsp |  microarray analysis |  highly virulent |  genome bacterial |  mediasiatica novicida
    KOL Index score: 14109

    The 16S ribosomal DNAs (rDNAs) of two strains of Francisella tularensis and one strain of Francisella philomiragia were sequenced. On the basis of phylogenetic analysis data, the genus Francisella was placed in the gamma subclass of the Proteobacteria. The most closely related organism was the intracellular bacterium Wolbachia persica. The sequenced 16S rDNA molecules of the Francisella species exhibited very high levels of similarity (98.5 to 99.9%). Two variable regions, comprising 390 ...

    Also Ranks for: Francisella Strains |  ribosomal dna |  species tularensis |  pcr genus |  data phylogeny
    KOL Index score: 13527

    Intracellular killing of Francisella tularensis by macrophages depends on interferon-gamma (IFN-gamma)-induced activation of the cells. The importance of inducible nitric oxide synthase (iNOS) or NADPH phagocyte oxidase (phox) for the cidal activity was studied. Murine IFN-gamma-activated peritoneal exudate cells (PEC) produced nitric oxide (NO), measured as nitrite plus nitrate, and superoxide. When PEC were infected with the live vaccine strain, LVS, of F. tularensis, the number of ...

    Also Ranks for: Tularensis Lvs |  oxygen species |  reactive nitrogen |  murine macrophages |  nitric oxide
    KOL Index score: 13149

    Migratory birds are known to play a role as long-distance vectors for many microorganisms. To investigate whether this is true of rickettsial agents as well, we characterized tick infestation and gathered ticks from 13,260 migratory passerine birds in Sweden. A total of 1127 Ixodes spp. ticks were removed from these birds and the extracted DNA from 957 of them was available for analyses. The DNA was assayed for detection of Rickettsia spp. using real-time PCR, followed by DNA sequencing ...

    Also Ranks for: Spotted Fever |  rickettsia spp |  migrating birds |  bird ticks |  greatest similarity
    KOL Index score: 13141

    Francisella tularensis subspecies tularensis is a highly virulent facultative intracellular pathogen of humans and a potential biological weapon. A live vaccine strain, F. tularensis LVS, was developed more than 50 years ago by pragmatic attenuation of a strain of the less virulent holarctica subspecies. LVS was demonstrated to be highly effective in human volunteers who were exposed to intradermal challenge with fully virulent subsp. tularensis, but was less effective against aerosol ...

    Also Ranks for: Aerosol Challenge |  tularensis strain |  schu s4 |  mice lvs |  tularemia vaccines
    KOL Index score: 12704

    The taxonomic status of the bacterium Wolbachia persica is described, and based on the evidence presented, transfer of this species to the genus Francisella as Francisella persica comb. nov. is proposed. This reclassification is supported by data generated from genomic comparisons of W. persica ATCC VR-331T ( = FSC845T = DSM 101678T) to other near neighbours, including Francisella tularensis subsp. novicida. The full-length 16S rRNA gene sequence of strain ATCC VR-331T had 98.5 % ...

    Also Ranks for: Francisella Persica |  tularensis subsp |  emended description |  wolbachia strain |  16s sequence analysis
    KOL Index score: 12375

    Reactive nitrogen species (RNS) and reactive oxygen species (ROS) are important mediators of the bactericidal host response. We investigated the contribution of these two mediators to the control of infection with the facultative intracellular bacterium Francisella tularensis. When intradermally infected with the live vaccine strain F. tularensis LVS, mice deficient in production of RNS (iNOS(-/-) mice) or in production of ROS by the phagocyte oxidase (p47(phox-/-) mice) showed ...

    Also Ranks for: Oxygen Species |  reactive nitrogen |  control infection |  rns ros |  distinct roles
    KOL Index score: 12199

    Previous studies have demonstrated that the four subspecies of the human pathogen Francisella tularensis, despite showing marked variations in their virulence for mammals and originating from different regions in the Northern Hemisphere, display a very close phylogenetic relationship. This property has hampered the development of generally applicable typing methods. To overcome this problem, we evaluated the use of PCR for discrimination of the subspecies using various forms of long ...

    Also Ranks for: Francisella Tularensis |  specific pcr |  dna bacterial |  northern hemisphere |  species subspecies
    KOL Index score: 12179

    Currently, there are no licensed vaccines and no correlates of protection against Francisella tularensis, which causes tularemia. We recently demonstrated that measuring in vitro control of intramacrophage bacterial growth by murine F. tularensis-immune splenocytes, as well as transcriptional analyses, discriminated Francisella vaccines of different efficacies. Further, we identified potential correlates of protection against systemic challenge. Here, we extended this approach by ...

    Also Ranks for: Francisella Tularensis |  vaccine efficacy |  gene expression |  potential correlates |  immune cells
    KOL Index score: 11423

    Francisella tularensis is a facultative intracellular bacterial pathogen capable of proliferating within host macrophages. The mechanisms that explain the differences in virulence between various strains of the species are not well characterized. In the present study, we show that both attenuated (strain LVS) and virulent (strains FSC200 and SCHU S4) strains of the pathogen replicate at similar rates in resting murine peritoneal exudate cells (PEC). However, when PEC were activated by ...

    Also Ranks for: Oxygen Species |  francisella tularensis |  reactive nitrogen |  schu s4 |  virulent strains
    KOL Index score: 11326

    Monocytic cells constitute an important defense mechanism against invading pathogens by recognizing conserved pathogens components. The recognition leads to activation of intracellular pathways involving nuclear factor kappa B (NF-kappaB) and mitogen-activated protein kinases (MAPK), such as the c-Jun NH2-terminal kinase (JNK), and p38. We show that in vitro infection with Francisella tularensis results in activation of NF-kappaB, phosphorylation of p38 and c-Jun, and secretion of ...

    Also Ranks for: Tularensis Lvs |  protein kinases |  human cells |  mitogen activated |  cytokine secretion
    KOL Index score: 11302

    Francisella tularensis subspecies tularensis is highly virulent for humans especially when it is inhaled. Therefore, it has the potential to be used as a biothreat agent. Vaccines against F. tularensis will need to be approved in accordance with the FDA Animal Rule. This will require identification of robust correlates of protection in experimental animals and the demonstration that similar immune responses are generated in vaccinated humans. Towards this goal, we have developed an ...

    Also Ranks for: Respiratory Challenge |  immune responses |  δclpb mutant |  inbred balb mice |  schu s4
    KOL Index score: 11238

    Microbial ligands, including lipopolysaccharide (LPS) and bacterial lipoproteins, activate Toll-like receptors (TLR) of mononuclear phagocytes, thereby inducing proinflammatory cytokines and antimicrobial activity. We show that Francisella tularensis, an intracellular pathogen, is capable of inhibiting this macrophage response. Infection with the live vaccine strain F. tularensis LVS rendered cells of the murine macrophage-like cell line J774A.1 incapable of secreting TNF-alpha or ...

    Also Ranks for: Francisella Tularensis |  antimicrobial activity |  j774 cells |  murine macrophages |  lvs lps
    KOL Index score: 11193

    Francisella tularensis subsp. tularensis (type A) strain SCHU S4 is a prototypic strain of the pathogen that is highly virulent for humans and other mammals. Its intradermal (i.d.) 50% lethal dose (LD50) for mice is <10 CFU. We discovered a spontaneous mutant, designated FSC043, of SCHU S4 with an i.d. LD50 of >10(8) CFU. FSC043 effectively vaccinated mice against challenge with a highly virulent type A strain, and the protective efficacy was at least as good as that of F. tularensis ...

    Also Ranks for: Schu S4 |  tularensis strain |  effective live vaccine |  deletion mutants |  protective efficacy


    Anders B Sjöstedt: Influence Statistics

    Sample of concepts for which Anders B Sjöstedt is among the top experts in the world.
    Concept World rank
    17kda protein mice #1
    virulence mechanisms review #1
    cytokines naive mice #1
    cysteine‐supplemented medium #1
    percentage vgamma9vdelta2 #1
    phagosomes phagosomal membrane #1
    effective live #1
    counteracts phagosomal #1
    mip1β tnf #1
    philomiragia strains #1
    escape tularensis #1
    t6ss essential #1
    t6s intracellular #1
    shell genomes #1
    fsc043 mutant #1
    persistent francisella #1
    iglc gene mutagenesis #1
    pcr processed tissues #1
    δoxyr lvs #1
    skin samples injection #1
    francisella immune #1
    nfkappab blp #1
    vacuoles lvs #1
    tularaemia vaccines lps #1
    lvs iglc #1
    human legionellosis #1
    vgrg core components #1
    tularensis function #1
    challenge inoculum #1
    sweden oropharyngeal tularaemia #1
    intracytosolic replication microinjection #1
    infection pronounced cytopathogenicity #1
    infected tularensis #1
    hmdms δpdpc mutant #1
    ∆clpb ∆wbtc splenocytes #1
    mice live tularensis #1
    rapid phagosomal escape #1
    unknown key elements #1
    oxyr katg #1
    protein crossreactive property #1
    francisella strains study #1
    skin tularemia #1
    154 samples patients #1
    francisella francisellaceae fam #1
    iglg igli #1
    bacterium intricate #1
    years tularemia #1
    chromosomal iglc gene #1
    pdpe pdpa #1
    burnetii differences #1

    Key People For Francisella Tularensis

    Top KOLs in the world
    Anders B Sjöstedt
    francisella tularensis schu s4 immune mice
    Richard W Titball
    yersinia pestis clostridium perfringens francisella tularensis
    Petra C F Oyston
    yersinia pestis francisella tularensis essential genes
    Arne Tärnvik
    francisella tularensis nephropathia epidemica puumala virus
    francisella tularensis schu s4 genome sequence
    Igor R Golovliov
    francisella tularensis schu s4 nitric oxide

    Anders B Sjöstedt:Expert Impact

    Concepts for whichAnders B Sjöstedthas direct influence:Francisella tularensis,  Tularensis subsp,  Schu s4,  Tularensis lvs,  Immune mice,  Tularensis strains,  Subspecies tularensis,  Murine macrophages.

    Anders B Sjöstedt:KOL impact

    Concepts related to the work of other authors for whichfor which Anders B Sjöstedt has influence:Francisella tularensis,  Schu s4,  Escherichia coli,  Virulence factors,  Live vaccine strain,  Causative agent,  Infectious diseases.



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    Department of Clinical Microbiology and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden | Umeå Centre for Microbial Research (UCMR) | Department of Clinical Microbiology, Umeå University, Umeå SE-90185, Sweden