Prominent publications by Kazumichi Furuyama

KOL Index score: 14959

Heme oxygenase 1 (HO-1) catalyzes heme breakdown, eventually releasing iron, carbon monoxide, and bilirubin IXalpha. HO-1 is induced by its substrate heme and various environmental factors, which represents a protective response against oxidative stresses. Here we show that hypoxia represses HO-1 expression in three human cell types but induces it in rat, bovine, and monkey cells, indicating the inter-species difference in the hypoxic regulation of HO-1 expression. The hypoxia-mediated ...

Known for Human Cells |  Expression Bach1 |  Heme Oxygenase |  Northern Blotting |  Genetic Rna
KOL Index score: 13463

DESIGN: It has recently been shown that deficiency of adrenomedullin (AM), a potent vasodilator peptide, leads to insulin resistance. We studied expression of AM in NIH 3T3-L1 adipocytes and compared it with expression of resistin, an adipocyte-derived peptide hormone that is proposed to cause insulin resistance. Moreover, we studied the effects of tumor necrosis factor-alpha (TNF-alpha), a known mediator of insulin resistance, on the expression of AM and resistin in 3T3-L1 ...

Known for Insulin Resistance |  L1 Adipocytes |  Necrosis Factor |  Expression Resistin |  3t3 Cells
KOL Index score: 11546

Heme oxygenase consists of two structurally related isozymes, heme oxygenase-1 and and heme oxygenase-2, each of which cleaves heme to form biliverdin, iron and carbon monoxide. Expression of heme oxygenase-1 is increased or decreased depending on cellular microenvironments, whereas little is known about the regulation of heme oxygenase-2 expression. Here we show that hypoxia (1% oxygen) reduces the expression levels of heme oxygenase-2 mRNA and protein after 48 h of incubation in human ...

Known for Heme Oxygenase |  Hepg2 Cells |  Expression Hypoxia |  Cell Lines |  Hela Cervical Cancer
KOL Index score: 11469

The retinal pigment epithelium (RPE) is essential for maintenance of photoreceptors and normally functions under conditions enriched with reactive oxygen species. RPE therefore expresses various defense enzymes against oxidative stress, including heme oxygenase-1 (HO-1). HO-1 catalyzes heme breakdown to release iron, carbon monoxide, and biliverdin, which is reduced to bilirubin, a potent radical scavenger. HO-1 expression is induced by various environmental factors, which has been ...

Known for Heme Oxygenase |  Epithelial Cells |  Hypoxia Expression |  Retinal Pigment |  Ho1 Gene
KOL Index score: 11335

Erythroid-specific 5-aminolevulinate synthase (ALAS2) is the rate-limiting enzyme for heme biosynthesis in erythroid cells, and a missense mutation of the ALAS2 gene is associated with congenital sideroblastic anemia. However, the gene responsible for this form of anemia remains unclear in about 40% of patients. Here, we identify a novel erythroid-specific enhancer of 130 base pairs in the first intron of the ALAS2 gene. The newly identified enhancer contains a cis-acting element that is ...

Known for Alas2 Gene |  Sideroblastic Anemia |  Erythroid Cells |  Function Mutation |  Heme Biosynthesis
KOL Index score: 11258

δ-Aminolevulinic acid (ALA)-induced porphyrin accumulation is widely used in the treatment of cancer, as photodynamic therapy. To clarify the mechanisms of the tumor-preferential accumulation of protoporphyrin, we examined the effect of the expression of heme-biosynthetic and -degradative enzymes on the ALA-induced accumulation of protoporphyrin as well as photodamage. The transient expression of heme-biosynthetic enzymes in HeLa cells caused variations of the ALA-induced accumulation of ...

Known for Tumor Cells |  Induced Accumulation |  Aminolevulinic Acid |  Protoporphyrin Photodamage |  Heme Oxygenase
KOL Index score: 10142

In eukaryotic cells, heme production is tightly controlled by heme itself through negative feedback-mediated regulation of nonspecific 5-aminolevulinate synthase (ALAS1), which is a rate-limiting enzyme for heme biosynthesis. However, the mechanism driving the heme-dependent degradation of the ALAS1 protein in mitochondria is largely unknown. In the current study, we provide evidence that the mitochondrial ATP-dependent protease ClpXP, which is a heteromultimer of CLPX and CLPP, is ...

Known for Heme Biosynthesis |  Alas1 Protein |  Mitochondrial Matrix |  5 Aminolevulinate |  Protease Clpxp
KOL Index score: 10124

Coproporphyrinogen oxidase (CPOX), the sixth enzyme in the heme-biosynthetic pathway, catalyzes oxidative decarboxylation of coproporphyrinogen to protoporphyrinogen and is located in the intermembrane space of mitochondria. To clarify the importance of CPOX in the regulation of heme biosynthesis in erythroid cells, we established human erythroleukemia K562 cells stably expressing mouse CPOX. The CPOX cDNA-transfected cells had sevenfold higher CPOX activity than cells transfected with ...

Known for K562 Cells |  Coproporphyrinogen Oxidase |  Expression Cpox |  Heme Biosynthesis |  Aminolevulinic Acid
KOL Index score: 9864

Sideroblastic anemia is characterized by anemia with the emergence of ring sideroblasts in the bone marrow. There are two forms of sideroblastic anemia, i.e., congenital sideroblastic anemia (CSA) and acquired sideroblastic anemia. In order to clarify the pathophysiology of sideroblastic anemia, a nationwide survey consisting of clinical and molecular genetic analysis was performed in Japan. As of January 31, 2012, data of 137 cases of sideroblastic anemia, including 72 cases of ...

Known for Sideroblastic Anemia |  Myelodysplastic Syndrome |  Ring Sideroblasts |  Csa Mdsrs |  Bone Marrow
KOL Index score: 9424

Intracellular heme concentrations are maintained in part by heme degradation, which is catalyzed by heme oxygenase. Heme oxygenase consists of two structurally related isozymes, HO-1 and HO-2. Recent studies have identified HO-2 as a potential oxygen sensor. To gain further insights into the regulatory role of HO-2 in heme homeostasis, we analyzed the expression profiles of HO-2 and the biochemical consequences of HO-2 knockdown with specific short interfering RNA (siRNA) in human cells. ...

Known for Heme Oxygenase |  Ho2 Expression |  Human Cell |  Messenger Rna |  Protein Levels
KOL Index score: 9363

Hypoxia induces expansion of erythroid precursor cells through erythropoietin production. However, it has also been suggested that hypoxia could enhance hemoglobin production in erythroid cells directly. To identify the molecules that are involved in hemoglobin production under hypoxia, we examined the expression profile of mRNAs in YN-1 human erythroleukemia cells under hypoxia. DNA array analysis revealed that the expression of transforming growth factor (TGF)-beta1 and mitoferrin, ...

Known for Erythroid Cells |  Hemoglobin Production |  Aminolevulinate Synthase |  Specific 5 |  Hypoxia Expression
KOL Index score: 8987

The erythroid transcription factor NF-E2 is an obligate heterodimer composed of two different subunits (p45 and p18), each containing a basic region-leucine zipper DNA binding domain, and it plays a critical role in erythroid differentiation as an enhancer-binding protein for expression of the beta-globin gene. We show here that dimethyl sulfoxide treatment of wild-type murine erythroleukemia cells, but not a mutant clone of dimethyl sulfoxide-resistant cells, increases NF-E2 activity ...

Known for Cell Differentiation |  Nf Transcription |  Binding Proteins |  Expression Regulation |  Factor P45
KOL Index score: 8936

GATA-1 is a transcription factor essential for erythroid cell development, and knockdown of GATA-1 gene results in maturation arrest and transformation of erythroblasts. To clarify the mechanism that gives rise to this abnormal phenotype, genes that are aberrantly expressed in the spleen of heterozygous GATA-1 knockdown mutant mouse (referred to as GATA-1 mutant mouse) were identified by using cDNA array. One of these genes, YB-1, was found to be highly expressed in the spleen of GATA-1 ...

Known for Gene Expression |  Transcription Factors |  Binding Proteins |  Erythroid Differentiation |  Gata1 Mutant Mouse
KOL Index score: 8451

Sideroblastic anemia is characterized by anemia with the emergence of ring sideroblasts in the bone marrow. Ring sideroblasts are erythroblasts characterized by iron accumulation in perinuclear mitochondria due to impaired iron utilization. There are two forms of sideroblastic anemia, i.e., inherited and acquired sideroblastic anemia. Inherited sideroblastic anemia is a rare and heterogeneous disease caused by mutations of genes involved in heme biosynthesis, iron–sulfur (Fe–S) cluster ...

Known for Sideroblastic Anemia |  Gene Mutations |  Heme Biosynthesis |  Ring Sideroblasts |  Erythroid Cells
KOL Index score: 7749

Heme plays an important biomodulating role in various cell functions. In this study, we examined the effects of hemin on cellular sensitivity to imatinib and other anti-leukemia reagents. Hemin treatment of human BCR/ABL-positive KCL22 leukemia cells increased IC(50) values of imatinib, that is, the drug resistance, in a dose-dependent manner without any change in the BCR/ABL kinase activity. Imatinib-induced apoptosis was also suppressed by hemin treatment in KCL22 cells. Hemin ...

Known for Leukemia Cells |  Cellular Sensitivity |  Human Bcr |  Nrf2 Expression |  Imatinibinduced Apoptosis


Kazumichi Furuyama: Influence Statistics

Sample of concepts for which Kazumichi Furuyama is among the top experts in the world.
Concept World rank
alas2 gene mutations #1
heme heme synthesis #1
governs cellular homeostasis #1
magnificent molecule #1
increased yn1 #1
alas1 ho1 #1
decreased hela #1
erythroid‐specific #1
r411c #1
sideroblastic anaemia patient #1
ho1 expression alas1 #1
sideroblastic anemia genes #1
heme fate #1
lines yn1 #1
heme determines #1
alas2 deficiency #1
cells yn1 #1
alas2 gene #1
hemeproteins homeostasis humans #1
hereditary sideroblastic anemia #1
heme alas1 #1
anaemia xlsa #1
alas1 #1
x‐linked sideroblastic #1
heme biosynthesis catabolism #1
alas mitochondria #1
yn1 cells #1
hememediated oxidization #1
yn1 0 #1
types sideroblastic anemia #1
heme biological events #1
gene products ring #1
erythropoiesis 5 #1
alas2 gene patients #2
potential oxygen sensor #2
expression mitoferrin mrna #2
x‐linked sideroblastic anaemia #2
induces erythroid‐specific #2
beta aminolevulinic #2
mitoferrin mrna #2
mouse cpox #2
fate heme #2
induces alas2 #2

Key People For Sideroblastic Anemia

Top KOLs in the world
Sylvia S Bottomley
sideroblastic anemia iron overload heme biosynthesis
David F Bishop
fabry disease uroporphyrinogen iii synthase acute intermittent porphyria
Alison May
sideroblastic anaemia prenatal diagnosis iron overload
Mark D Fleming
sideroblastic anemia iron deficiency myelodysplastic syndrome
Philip D Cotter
situ hybridization prenatal diagnosis human pair
Dean R Campagna
sideroblastic anemia gata2 mutations iron deficiency

Kazumichi Furuyama:Expert Impact

Concepts for whichKazumichi Furuyamahas direct influence:Sideroblastic anemia,  K562 cells,  Alas2 gene,  Heme oxygenase,  Coproporphyrinogen oxidase,  Erythroid cells,  Heme biosynthesis,  Hypoxia expression.

Kazumichi Furuyama:KOL impact

Concepts related to the work of other authors for whichfor which Kazumichi Furuyama has influence:Heme oxygenase,  Sideroblastic anemia,  Erythroid cells,  Carbon monoxide,  Iron metabolism,  Ring sideroblasts,  Aminolevulinic acid.



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Department of Molecular Biochemistry, Iwate Medical University School of Medicine, Yahaba, Iwate, Japan | Department of Molecular Biochemistry, Iwate Medical University, Nishitokuta 2-1-1, 028-3694, Yahaba, Iwate, Japan | Department of Molecular Bioc

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