• KOL
    • Somatic Embryogenesis
    • Indra K Vasil
    • Indra K Vasil: Influence Statistics

      Indra K Vasil

      Indra K Vasil

      University of Florida, Gainesville, FL 32611-0690, USA | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA | University of Florida, Box 110690, 32611-0690, ...

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      Indra K Vasil:Expert Impact

      Concepts for whichIndra K Vasilhas direct influence:Somatic embryogenesis,Pennisetum americanum,Pearl millet,Plant regeneration,Guinea grass,Pennisetum purpureum,Napier grass,Plant biotechnology.

      Indra K Vasil:KOL impact

      Concepts related to the work of other authors for whichfor which Indra K Vasil has influence:Somatic embryogenesis,Plant regeneration,Gene expression,Abscisic acid,Tissue culture,Embryogenic callus,Immature embryos.

      KOL Resume for Indra K Vasil

      Year
      2009

      University of Florida, Gainesville, FL 32611-0690, USA

      2008

      University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA

      2007

      University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA

      2006

      Laboratory of Plant Cell and Molecular Biology, University of Florida, Gainesville, USA.

      2005

      Laboratory of Plant Cell and Molecular Biology, 1143 Fifield Hall, Box 110690, University of Florida, Gainesville, FL 32611-0690

      Author to whom correspondence should be addressed:, ikv, ifas.ufl.edu

      2003

      the University of Florida, Box 110690, 32611-0690, Gainesville, Florida, USA

      2002

      Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, Box 110690, 32611-0690, Gainesville, FL

      2001

      Laboratory of Plant Cell and Molecular Biology, 1143 Fifield Hall, University of Florida, Gainesville, FL 32611-0690, USA

      1999

      Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, 32611-0690, Gainesville, FL, USA

      1998

      graduate research professor in the Laboratory of Plant Cell and Molecular Biology, University of Florida, 32611-0690, Gainesville, FL

      1997

      Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Gainesville, Florida, USA

      1996

      Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA

      graduate research professor in the laboratory of plant cell and molecular biology, University of Florida, FL32611 -0690, Gainesville

      1995

      Horticultural Sciences Department, University of Florida, Gainesville 32611

      1994

      Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, 32611-0692, Gainesville, Florida, USA

      1993

      Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 1143 Fifield Hall, 32611, Gainesville, FL, USA

      1992

      Laboratory of Plant Cell and Molecular Biology, University of Florida, 1253 Fifield Hall, 32611, Gainesville, FL

      Vegetable Crops Department, University of Florida, Gainesville 32611.

      1991

      Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL

      Vegetable Crops Department University of Florida Gainesville, Florida 32611, USA

      1990

      graduate research professor in the laboratory of plant cell and molecular biology , department of vergetable crops, University of Florida, 32611-0514, Gainesville, FL

      University of Florida in Gainesville, USA

      1989

      Department of Vegetable Crops, University of Florida, Gainesville, Florida 32611

      1988

      Plant Biotech Industries Ltd., Mobile Post, 25201, Ashrat, Israel

      Department of Vegetable Crops, University of Florida, Gainesville, FL 32611, USA

      1987

      Department of Botany, University of Florida, Gainesville, FL 32611,

      1986

      Department of Botany, University of Florida, 32611, Gainesville, Florida, USA

      1985

      Department of Botany, University of Florida, FL 32611, Gainesville, USA

      1984

      Department of Botany, University of Florida, Gainesville, FL 32611, USA

      1983

      Department of Botany, University of Florida, FL32611, Gainesville, USA

      1982

      Department of Botany, University of Florida, Gainesville, FL 32611 (U.S.A.)

      1981

      Department of Botany, University of Florida, Gainesville, USA

      1980

      Department of Botany, University of Florida, Gainesville, Florida, USA

      1979

      University of Florida, Gainesville, Florida, USA

      1978

      Department of Botany, University of Florida, Gainesville, 32611

      1977

      University of Florida, 32611, Gainesville, Florida, USA

      1976

      Dept. of Botany, University of Florida, 32611, Gainesville, Florida

      1975

      Physiologie Pluricellulaire, C.N.R.S., Gif-sur-Yvette, France

      1974

      Department of Botany, University of Florida, 32611, Gainesville, Florida

      1973

      Department of Botany, University of Florida, Gainesville, Florida 32601 (U.S.A.).

      1970

      Department of Botany, University of Florida, Gainesville, Florida 32601, USA

      1967

      Department of Botany, University of Florida, Ganesville, Florida, U.S.A.

      1966

      Division of Botany, Indian Agricultural Research Institute, Neu Delhi-12, India

      Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin, USA

      1964

      Department of Botany, University of Illinois, Urbana, Ill. U.S.A.

      1961

      Botany Department, University of Florida, Gainesville, Florida

      University of Delhi, India

      1960

      Department of Botany, University of Delhi, Delhi 6, India

      1959

      Department of Botany, University of Delhi, Delhi, India

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      Sample of concepts for which Indra K Vasil is among the top experts in the world.
      Concept World rank
      suspension maintenance medium #1
      callus year #1
      chlorophyllous callus tissues #1
      2–6 cells #1
      embryoid plantlet formation #1
      protoplasts cereal species #1
      suspension maize #1
      artificial nutrient media #1
      1 mesophyll protoplasts #1
      eventually degeneratein situ #1
      loci somatic embryos #1
      plant genome regeneration #1
      cultured shoot segments #1
      protoplasts pennisetum #1
      cichorium endivia linn #1
      low 24d medium #1
      somatic embryos rachis #1
      plant callus tissues #1
      einkorn embryogenic suspension #1
      light embryoids #1
      tissue culture11journal #1
      75 plastic #1
      leaf segments panicum #1
      meristem lupinus #1
      plants r0 #1
      qualitative genetic markers #1
      morphological evidence zones #1
      biology ofazospirillum #1
      soft unorganized callus #1
      food products kinds #1
      nptii kanamycin #1
      r0 plants lines #1
      cassette intron #1
      wheat defined medium #1
      plants tolerant calli #1
      callus direct selection #1
      aged friable calli #1
      differences callus formation #1
      tissue cultures organogenesis #1
      nuclei microspore tetrads #1
      length murashige #1
      perennial structures #1
      1ax1 gene wheat #1
      applications plant biotechnology #1
      Sign-in to see all concepts, it's free!

      Prominent publications by Indra K Vasil

      KOL-Index: 15842

      Effects of application in vivo of glyphosate, fluridone, and paclobutrazol to glasshouse-grown donor plants of Pennisetum purpureum Schum. on endogenous levels of abscisic acid (ABA) and indole-3-acetic acid (IAA) in young leaves and on somatic embryogenesis in cultured leaf explants were studied. Treatment of plants with glyphosate (100 milligrams per liter) resulted in elevated levels of endogenous ABA and IAA in young leaves. In contrast, paclobutrazol (50% active ingredient; 200 ...

      Known for Somatic Embryogenesis | Leaf Explants | Abscisic Acid | Pennisetum Purpureum | Aba Iaa
      KOL-Index: 10004

      The unique breadmaking properties of wheat are closely related to the quality and quantity of high molecular weight (HMW) glutenins present in wheat flour. We have produced several transgenic wheat lines expressing the high molecular weight glutenin subunit (HMW-GS) gene 1Ax1. They were analyzed for stability of gene expression and the effect of over-expressed 1Ax1 protein on protein composition, agronomic traits and flour functionality in R4 seeds obtained from plants grown in the ...

      Known for Molecular Weight | Transgenic Wheat | Protein Composition | Expression 1ax1 | Field Grown
      KOL-Index: 8887

      We have developed an improved protocol for the rapid and efficient production of transgenic wheat. Three plasmids, each containing the selectable bar gene for resistance to the herbicide Basta and the β-glucuronidase (GUS) reporter gene, were delivered via particle bombardment, directly into immature embryos of two spring and one winter cultivar of wheat four days to two months after culture. Resistant calli were selected on phosphinothricin (PPT) media and screened for histochemical GUS ...

      Known for Immature Embryos | Transgenic Wheat | Rapid Production | Bar Gene | R0 Plants
      KOL-Index: 8455

      The C1 regulatory gene of the maize anthocyanin pathway is regulated by a combination of developmental and environmental signals that include the Viviparous1 (Vp1) gene, abscisic acid (ABA), and light. Using protoplast electroporation and particle bombardment assays, we have defined c/s-acting elements that are necessary and sufficient for the activation of C1 by ABA, VP1, and light, respectively. The sequence from positions -142 to -132 (CGTCCATGCAT) is essential for VP1 activation, ...

      Known for Abscisic Acid | Plant Genes | Acting Elements | Vp1 Activation | Expression Regulation
      KOL-Index: 8085

      Tissue cultures ofTriticum aestivum L. (wheat) initiated from young inflorescences and immature embryos possessed the potential for regeneration of whole plants. Both a friable and a compact type of callus were produced on Murashige and Skoog's medium with 2 mg/l 2,4-dichlorophenoxyacetic acid. The friable callus contained meristematic centers in which the peripheral cells ceased dividing, elongated, and could be easily separated. Roots were frequently formed in this type of callus. The ...

      Known for Immature Embryo | Plant Regeneration | Compact Callus | Oftriticum Aestivum | Smooth Surface
      KOL-Index: 8044

      Somatic hybrid cell lines with embryogenic capacity were obtained by fusion of protoplasts isolated from an embryogenic cell line of sugarcane with inactivated protoplasts of an S-(2-aminoethyl)-L-cysteine (Aet-Cys)-resistant cell line of pearl millet. Initial selection for putative hybrids was performed by plating protoplast-derived microcolonies onto medium containing 300 mug of Aet-Cys per ml. Calli growing in the presence of Aet-Cys were screened with respect to their electrophoretic ...

      Known for Pearl Millet | Somatic Hybrid | Pennisetum Americanum | Saccharum Officinarum | Cell Lines
      KOL-Index: 7939

      Protoplasts isolated enzymatically from leaves of tobacco (Nicotiana tabacum), stem callus of petunia (Petunia hybrida) and roots of corn (Zea mays) seedings were grown aseptically in microchambers, liquid droplets, or in stationary suspension cultures. Cell wall regenration occurred within 2 days in petunia, 6 to 7 days in tobacco, and 10 days in corn protoplasts. In petunia protoplasts, cell divisions ensued immediately after cell wall formation, and a colony of 30 to 40 cells was ...

      Known for Tobacco Protoplasts | Cell Wall | Plants Derived | Petunia Hybrida | Plant Development
      KOL-Index: 7690

      Endogenous levels of major plant growth regulators were measured in leaves and tissue cultures of Napiergrass (Pennisetum purpureum Schum.) either by high-performance liquid chromatography or radioimmunoassay. The developmentally young, basal region of the tightly furled innermost four young leaves are highly embryogenic (45% of explants produce embryogenic callus) as compared to the more mature middle (28%) and distal (5%) parts of the same leaves. The basal region contained, on a ...

      Known for Embryogenic Callus | Tissue Cultures | Growth Regulators | Endogenous Levels | Young Leaves
      KOL-Index: 7687

      The relationship between promoter sequences that mediate Viviparous1 (VP1) transactivation and regulation by abscisic acid (ABA) in the wheat Em promoter was investigated using deletion analysis and directed mutagenesis. The Em1a G-box is strongly coupled to VP1 transactivation as well as to ABA regulation; however, the Em promoter includes additional components that can support VP1 transactivation without ABA responsiveness or synergism. Oligonucleotide tetramers of several G-box ...

      Known for Abscisic Acid | Vp1 Transactivation | Aba Regulation | Promoter Sequences | Response Elements
      KOL-Index: 7397

      Protoplasts from Pennisetum americanum resistant to S-2-amino-ethyl-l-cysteine (AEC) were fused with protoplasts of Panicum maximum utilizing polyethylene glycol-dimethylsulfoxide after inactivation of the Pennisetum protoplasts with 1 mM iodoacetic acid. The iodoacetate treatment prevented division of Pennisetum protoplasts; therefore, only Panicum protoplasts and heterokaryons potentially could give rise to colonies. A second level of selection was imposed by plating 3–4-week-old ...

      Known for Pennisetum Americanum | Somatic Hybridization | Pearl Millet | Panicum Maximum | Hybrid Lines
      KOL-Index: 7345

      The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cultivars depends on the number and composition of the HMW-GS present. We have introduced the HMW-GS 1Ax1 gene, known to be associated with good bread-making quality, into the ...

      Known for Quality Wheat | Weight Glutenin | Transgenic Lines | Gluten Proteins | Genetic Triticum
      KOL-Index: 7310

      Transient GUS (β-glucuronidase) expression was visualized in cell suspensions of Triticum aestivum, Zea mays, Pennisetum glaucum, Saccharum officinarum, Pennisetum purpureum and Panicum maximum after microprojectile bombardment with pBARGUS and pAHC25 plasmid DNAs. pBARGUS contains the GUS (UidA) gene coding region driven by the Adh1 promoter and the Adh1 intron 1, as well as the BAR gene coding region driven by the CaMV 35S promoter and the Adh1 intron 1. pAHC25 contains the GUS and BAR ...

      Known for Gene Expression | Immature Embryos | Triticum Aestivum | Cell Suspension | Pennisetum Glaucum

      Key People For Somatic Embryogenesis

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      University of Florida, Gainesville, FL 32611-0690, USA | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA | Laboratory of Plant Cell and Molecular Biology, Unive

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