 | Indra K VasilUniversity of Florida, Gainesville, FL 32611-0690, USA | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA | University of Florida, Box 110690, 32611-0690, ... |
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Indra K Vasil:Expert Impact
Concepts for whichIndra K Vasilhas direct influence:Somatic embryogenesis,Pennisetum americanum,Pearl millet,Plant regeneration,Guinea grass,Pennisetum purpureum,Napier grass,Plant biotechnology.
Indra K Vasil:KOL impact
Concepts related to the work of other authors for whichfor which Indra K Vasil has influence:Somatic embryogenesis,Plant regeneration,Gene expression,Abscisic acid,Tissue culture,Embryogenic callus,Immature embryos.
KOL Resume for Indra K Vasil
| Year | |
|---|---|
| 2009 | University of Florida, Gainesville, FL 32611-0690, USA |
| 2008 | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA |
| 2007 | University of Florida, Box 110690, 32611-0690, Gainesville, FL, USA |
| 2006 | Laboratory of Plant Cell and Molecular Biology, University of Florida, Gainesville, USA. |
| 2005 | Laboratory of Plant Cell and Molecular Biology, 1143 Fifield Hall, Box 110690, University of Florida, Gainesville, FL 32611-0690 Author to whom correspondence should be addressed:, ikv, ifas.ufl.edu |
| 2003 | the University of Florida, Box 110690, 32611-0690, Gainesville, Florida, USA |
| 2002 | Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, Box 110690, 32611-0690, Gainesville, FL |
| 2001 | Laboratory of Plant Cell and Molecular Biology, 1143 Fifield Hall, University of Florida, Gainesville, FL 32611-0690, USA |
| 1999 | Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, 32611-0690, Gainesville, FL, USA |
| 1998 | graduate research professor in the Laboratory of Plant Cell and Molecular Biology, University of Florida, 32611-0690, Gainesville, FL |
| 1997 | Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Gainesville, Florida, USA |
| 1996 | Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Forida, 32611-0690, Gainesville, Florida, USA graduate research professor in the laboratory of plant cell and molecular biology, University of Florida, FL32611 -0690, Gainesville |
| 1995 | Horticultural Sciences Department, University of Florida, Gainesville 32611 |
| 1994 | Laboratory of Plant Cell and Molecular Biology, University of Florida, 1143 Fifield Hall, 32611-0692, Gainesville, Florida, USA |
| 1993 | Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 1143 Fifield Hall, 32611, Gainesville, FL, USA |
| 1992 | Laboratory of Plant Cell and Molecular Biology, University of Florida, 1253 Fifield Hall, 32611, Gainesville, FL Vegetable Crops Department, University of Florida, Gainesville 32611. |
| 1991 | Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL Vegetable Crops Department University of Florida Gainesville, Florida 32611, USA |
| 1990 | graduate research professor in the laboratory of plant cell and molecular biology , department of vergetable crops, University of Florida, 32611-0514, Gainesville, FL University of Florida in Gainesville, USA |
| 1989 | Department of Vegetable Crops, University of Florida, Gainesville, Florida 32611 |
| 1988 | Plant Biotech Industries Ltd., Mobile Post, 25201, Ashrat, Israel Department of Vegetable Crops, University of Florida, Gainesville, FL 32611, USA |
| 1987 | Department of Botany, University of Florida, Gainesville, FL 32611, |
| 1986 | Department of Botany, University of Florida, 32611, Gainesville, Florida, USA |
| 1985 | Department of Botany, University of Florida, FL 32611, Gainesville, USA |
| 1984 | Department of Botany, University of Florida, Gainesville, FL 32611, USA |
| 1983 | Department of Botany, University of Florida, FL32611, Gainesville, USA |
| 1982 | Department of Botany, University of Florida, Gainesville, FL 32611 (U.S.A.) |
| 1981 | Department of Botany, University of Florida, Gainesville, USA |
| 1980 | Department of Botany, University of Florida, Gainesville, Florida, USA |
| 1979 | University of Florida, Gainesville, Florida, USA |
| 1978 | Department of Botany, University of Florida, Gainesville, 32611 |
| 1977 | University of Florida, 32611, Gainesville, Florida, USA |
| 1976 | Dept. of Botany, University of Florida, 32611, Gainesville, Florida |
| 1975 | Physiologie Pluricellulaire, C.N.R.S., Gif-sur-Yvette, France |
| 1974 | Department of Botany, University of Florida, 32611, Gainesville, Florida |
| 1973 | Department of Botany, University of Florida, Gainesville, Florida 32601 (U.S.A.). |
| 1970 | Department of Botany, University of Florida, Gainesville, Florida 32601, USA |
| 1967 | Department of Botany, University of Florida, Ganesville, Florida, U.S.A. |
| 1966 | Division of Botany, Indian Agricultural Research Institute, Neu Delhi-12, India Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin, USA |
| 1964 | Department of Botany, University of Illinois, Urbana, Ill. U.S.A. |
| 1961 | Botany Department, University of Florida, Gainesville, Florida University of Delhi, India |
| 1960 | Department of Botany, University of Delhi, Delhi 6, India |
| 1959 | Department of Botany, University of Delhi, Delhi, India |
| Concept | World rank |
|---|---|
| suspension maintenance medium | #1 |
| callus year | #1 |
| chlorophyllous callus tissues | #1 |
| 2–6 cells | #1 |
| embryoid plantlet formation | #1 |
| protoplasts cereal species | #1 |
| suspension maize | #1 |
| artificial nutrient media | #1 |
| 1 mesophyll protoplasts | #1 |
| eventually degeneratein situ | #1 |
| loci somatic embryos | #1 |
| plant genome regeneration | #1 |
| cultured shoot segments | #1 |
| protoplasts pennisetum | #1 |
| cichorium endivia linn | #1 |
| low 24d medium | #1 |
| somatic embryos rachis | #1 |
| plant callus tissues | #1 |
| einkorn embryogenic suspension | #1 |
| light embryoids | #1 |
| tissue culture11journal | #1 |
| 75 plastic | #1 |
| leaf segments panicum | #1 |
| meristem lupinus | #1 |
| plants r0 | #1 |
| qualitative genetic markers | #1 |
| morphological evidence zones | #1 |
| biology ofazospirillum | #1 |
| soft unorganized callus | #1 |
| food products kinds | #1 |
| nptii kanamycin | #1 |
| r0 plants lines | #1 |
| cassette intron | #1 |
| wheat defined medium | #1 |
| plants tolerant calli | #1 |
| callus direct selection | #1 |
| aged friable calli | #1 |
| differences callus formation | #1 |
| tissue cultures organogenesis | #1 |
| nuclei microspore tetrads | #1 |
| length murashige | #1 |
| perennial structures | #1 |
| 1ax1 gene wheat | #1 |
| applications plant biotechnology | #1 |
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Prominent publications by Indra K Vasil
Effects of application in vivo of glyphosate, fluridone, and paclobutrazol to glasshouse-grown donor plants of Pennisetum purpureum Schum. on endogenous levels of abscisic acid (ABA) and indole-3-acetic acid (IAA) in young leaves and on somatic embryogenesis in cultured leaf explants were studied. Treatment of plants with glyphosate (100 milligrams per liter) resulted in elevated levels of endogenous ABA and IAA in young leaves. In contrast, paclobutrazol (50% active ingredient; 200 ...
| Known for Somatic Embryogenesis | Leaf Explants | Abscisic Acid | Pennisetum Purpureum | Aba Iaa |
The unique breadmaking properties of wheat are closely related to the quality and quantity of high molecular weight (HMW) glutenins present in wheat flour. We have produced several transgenic wheat lines expressing the high molecular weight glutenin subunit (HMW-GS) gene 1Ax1. They were analyzed for stability of gene expression and the effect of over-expressed 1Ax1 protein on protein composition, agronomic traits and flour functionality in R4 seeds obtained from plants grown in the ...
| Known for Molecular Weight | Transgenic Wheat | Protein Composition | Expression 1ax1 | Field Grown |
Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos
[ PUBLICATION ]
We have developed an improved protocol for the rapid and efficient production of transgenic wheat. Three plasmids, each containing the selectable bar gene for resistance to the herbicide Basta and the β-glucuronidase (GUS) reporter gene, were delivered via particle bombardment, directly into immature embryos of two spring and one winter cultivar of wheat four days to two months after culture. Resistant calli were selected on phosphinothricin (PPT) media and screened for histochemical GUS ...
| Known for Immature Embryos | Transgenic Wheat | Rapid Production | Bar Gene | R0 Plants |
Localization and interaction of the cis-acting elements for abscisic acid, VIVIPAROUS1, and light activation of the C1 gene of maize.
[ PUBLICATION ]
The C1 regulatory gene of the maize anthocyanin pathway is regulated by a combination of developmental and environmental signals that include the Viviparous1 (Vp1) gene, abscisic acid (ABA), and light. Using protoplast electroporation and particle bombardment assays, we have defined c/s-acting elements that are necessary and sufficient for the activation of C1 by ABA, VP1, and light, respectively. The sequence from positions -142 to -132 (CGTCCATGCAT) is essential for VP1 activation, ...
| Known for Abscisic Acid | Plant Genes | Acting Elements | Vp1 Activation | Expression Regulation |
Plant regeneration from cultured immature embryos and inflorescences ofTriticum aestivum L. (wheat): Evidence for somatic embryogenesis
[ PUBLICATION ]
Tissue cultures ofTriticum aestivum L. (wheat) initiated from young inflorescences and immature embryos possessed the potential for regeneration of whole plants. Both a friable and a compact type of callus were produced on Murashige and Skoog's medium with 2 mg/l 2,4-dichlorophenoxyacetic acid. The friable callus contained meristematic centers in which the peripheral cells ceased dividing, elongated, and could be easily separated. Roots were frequently formed in this type of callus. The ...
| Known for Immature Embryo | Plant Regeneration | Compact Callus | Oftriticum Aestivum | Smooth Surface |
Somatic hybridization in the Gramineae: Saccharum officinarum L. (sugarcane) and Pennisetum americanum (L.) K. Schum. (pearl millet)
[ PUBLICATION ]
Somatic hybrid cell lines with embryogenic capacity were obtained by fusion of protoplasts isolated from an embryogenic cell line of sugarcane with inactivated protoplasts of an S-(2-aminoethyl)-L-cysteine (Aet-Cys)-resistant cell line of pearl millet. Initial selection for putative hybrids was performed by plating protoplast-derived microcolonies onto medium containing 300 mug of Aet-Cys per ml. Calli growing in the presence of Aet-Cys were screened with respect to their electrophoretic ...
| Known for Pearl Millet | Somatic Hybrid | Pennisetum Americanum | Saccharum Officinarum | Cell Lines |
Regeneration of tobacco and petunia plants from protoplasts an culture of corn protoplasts
[ PUBLICATION ]
Protoplasts isolated enzymatically from leaves of tobacco (Nicotiana tabacum), stem callus of petunia (Petunia hybrida) and roots of corn (Zea mays) seedings were grown aseptically in microchambers, liquid droplets, or in stationary suspension cultures. Cell wall regenration occurred within 2 days in petunia, 6 to 7 days in tobacco, and 10 days in corn protoplasts. In petunia protoplasts, cell divisions ensued immediately after cell wall formation, and a colony of 30 to 40 cells was ...
| Known for Tobacco Protoplasts | Cell Wall | Plants Derived | Petunia Hybrida | Plant Development |
Endogenous Growth Regulators in Leaves and Tissue Cultures of Pennisetum purpureum Schum.
[ PUBLICATION ]
Endogenous levels of major plant growth regulators were measured in leaves and tissue cultures of Napiergrass (Pennisetum purpureum Schum.) either by high-performance liquid chromatography or radioimmunoassay. The developmentally young, basal region of the tightly furled innermost four young leaves are highly embryogenic (45% of explants produce embryogenic callus) as compared to the more mature middle (28%) and distal (5%) parts of the same leaves. The basal region contained, on a ...
| Known for Embryogenic Callus | Tissue Cultures | Growth Regulators | Endogenous Levels | Young Leaves |
The relationship between promoter sequences that mediate Viviparous1 (VP1) transactivation and regulation by abscisic acid (ABA) in the wheat Em promoter was investigated using deletion analysis and directed mutagenesis. The Em1a G-box is strongly coupled to VP1 transactivation as well as to ABA regulation; however, the Em promoter includes additional components that can support VP1 transactivation without ABA responsiveness or synergism. Oligonucleotide tetramers of several G-box ...
| Known for Abscisic Acid | Vp1 Transactivation | Aba Regulation | Promoter Sequences | Response Elements |
Somatic hybridization in the gramineae: Pennisetum americanum (L.) K. Schum. (Pearl millet) +Panicum maximum Jacq. (Guinea grass)
[ PUBLICATION ]
Protoplasts from Pennisetum americanum resistant to S-2-amino-ethyl-l-cysteine (AEC) were fused with protoplasts of Panicum maximum utilizing polyethylene glycol-dimethylsulfoxide after inactivation of the Pennisetum protoplasts with 1 mM iodoacetic acid. The iodoacetate treatment prevented division of Pennisetum protoplasts; therefore, only Panicum protoplasts and heterokaryons potentially could give rise to colonies. A second level of selection was imposed by plating 3–4-week-old ...
| Known for Pennisetum Americanum | Somatic Hybridization | Pearl Millet | Panicum Maximum | Hybrid Lines |
Integration and expression of the high-molecular-weight glutenin subunit 1Ax1 gene into wheat
[ PUBLICATION ]
The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cultivars depends on the number and composition of the HMW-GS present. We have introduced the HMW-GS 1Ax1 gene, known to be associated with good bread-making quality, into the ...
| Known for Quality Wheat | Weight Glutenin | Transgenic Lines | Gluten Proteins | Genetic Triticum |
Enhanced GUS gene expression in cereal/grass cell suspensions and immature embryos using the maize uhiquitin-based plasmid pAHC25
[ PUBLICATION ]
Transient GUS (β-glucuronidase) expression was visualized in cell suspensions of Triticum aestivum, Zea mays, Pennisetum glaucum, Saccharum officinarum, Pennisetum purpureum and Panicum maximum after microprojectile bombardment with pBARGUS and pAHC25 plasmid DNAs. pBARGUS contains the GUS (UidA) gene coding region driven by the Adh1 promoter and the Adh1 intron 1, as well as the BAR gene coding region driven by the CaMV 35S promoter and the Adh1 intron 1. pAHC25 contains the GUS and BAR ...
| Known for Gene Expression | Immature Embryos | Triticum Aestivum | Cell Suspension | Pennisetum Glaucum |
