 | Zhikang Peng |
Prominent publications by Zhikang Peng
BACKGROUND: Allergic reactions to mosquito bites are a common problem. Although IgE-mediated hypersensitivity has been reported, other immunologic mechanisms may be involved.
OBJECTIVES: To study the relationship between skin bite reactions and immunologic parameters.
METHODS: Forty-one subjects were experimentally exposed to mosquito (Aedes vexans) bites. Immediate and delayed skin reactions were traced at 20 minutes and 24 hours, respectively, after the bites. Sera were analyzed for ...
| Also Ranks for: Skin Reactions | mosquito allergy | specific ige | igg antibodies | lymphocyte proliferation |
BACKGROUND: Most people develop skin reactions to mosquito bites, however, little is known about mosquito salivary allergens and the IgE responses to them.
OBJECTIVES: We sought to identify these allergens and the specific IgE responses they elicit.
METHODS: Saliva or salivary gland extracts were prepared from 10 mosquito species, including seven species with worldwide distribution: Aedes (Ae.) aegypti, Ae. vexans, Ae. albopictus, Ae. togoi, Ae. triseriatus, Culex (Cx.) quinquefasciatus, ...
| Also Ranks for: Ige Responses | mosquito species | salivary allergens | immunoblot analysis | skin reactions |
Accurate diagnosis of mosquito allergy has been precluded by the difficulty of obtaining salivary allergens. In this study, we expressed, purified, characterized and investigated the clinical relevance of a recombinant Aedes aegypti salivary allergen, rAed a 1. Two cDNA segments were ligated together to form the full-length Aed a 1 gene. rAed a 1 was expressed using a baculovirus/insect cell system, and purified using a combination of anion-exchange and gel-filtration chromatography. The ...
| Also Ranks for: Salivary Allergen | aedes aegypti | recombinant mosquito | delayed hypersensitivity | positive raed |
All‐trans retinoic acid down‐regulates inflammatory responses by shifting the Treg/Th17 profile in human ulcerative and murine colitis
[ PUBLICATION ]
IBD is characterized by uncontrolled immune responses in inflamed mucosa, with dominance of IL-17-producing cells and deficiency of Treg cells. The aim of this study was to explore the effect and mechanisms of RA, the ligand of RARalpha, on immune responses in human and murine colitis. Colonic biopsies from patients with UC were cultured and treated with RA as the agonist of RARalpha or LE135 as the antagonist of RARalpha. Expressions of IL-17 and FOXP3 were detected by ...
| Also Ranks for: Murine Colitis | inflammatory responses | retinoic acid | patients ibd | colon tissues |
AMPK agonist downregulates innate and adaptive immune responses in TNBS-induced murine acute and relapsing colitis
[ PUBLICATION ]
AMP-activated protein kinase (AMPK), a cellular energy sensor, has been reported to participate in modulating inflammatory responses, but its role in intestinal inflammation remains unclear. IBD has been characterized by excessive innate and adaptive immune responses. Here, the roles of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), an agonist of AMPK, in regulating immune responses of experimental colitis were investigated. In vitro effects of AICAR on LPS-induced macrophage ...
| Also Ranks for: Immune Responses | inbred balb mice | experimental colitis | treatment ibd | ampk aicar |
Immune responses to mosquito saliva in 14 individuals with acute systemic allergic reactions to mosquito bites
[ PUBLICATION ]
BACKGROUND: Mosquito bite-induced acute systemic allergic reactions are an increasing clinical concern and have not been optimally characterized immunologically.
OBJECTIVE: We wanted to study the immunologic basis of these reactions.
METHODS: Sera were received from 14 individuals with a history of acute systemic allergic reactions to mosquito bites, defined as the presence of one or more of the following: urticaria, angioedema, wheezing, dyspnea, hypotension, and decrease or loss of ...
| Also Ranks for: Mosquito Bites | allergic reactions | immune responses | aedes albopictus | specific ige |
Targeting IL-12/IL-23 by Employing a p40 Peptide-Based Vaccine Ameliorates TNBS-Induced Acute and Chronic Murine Colitis
[ PUBLICATION ]
Interleukin (IL)-12 and IL-23 both share the p40 subunit and are key cytokines in the pathogenesis of Crohn’s disease. Previously, we have developed and identified three mouse p40 peptide-based and virus-like particle vaccines. Here, we evaluated the effects and immune mechanisms of the optimal vaccine in downregulating intestinal inflammation in murine acute and chronic colitis, induced by intrarectal administrations of trinitrobenzene sulfonic acid (TNBS). Mice were injected ...
| Also Ranks for: Chronic Colitis | intestinal inflammation | crohn disease | based vaccine | vaccinated mice |
Induction of IgE Responses Using a Recombinant Mosquito Salivary Allergen rAed a 2 without Adjuvant in Mice
[ PUBLICATION ]
BACKGROUND: Reactions to mosquito bites are a global problem. Several salivary proteins from Aedes (Ae.) aegypti, the most common mosquito species, have been cloned and expressed. Plasmid DNA vaccination has been shown to be effective in the downregulation of IgE responses. To investigate the in vivo antigenicity of these recombinant proteins and to study the mechanisms underlying plasmid DNA vaccination, a mouse model sensitized with a recombinant antigen has been developed.
METHODS: ...
| Also Ranks for: Ige Responses | mosquito salivary | raed 2 | recombinant proteins | immunologic aedes |
Cross-reactivity of skin and serum specific IgE responses and allergen analysis for three mosquito species with worldwide distribution
[ PUBLICATION ]
BACKGROUND: Allergic reactions to mosquito bites are a common problem worldwide. Cross-reactive immunologic responses have not been reported.
OBJECTIVE: For the three most common mosquito species, Aedes (Ae.) vexans, Ae. aegypti, and Culex (Cx.) quinquefaciatus, we investigated skin and serum specific IgE responses and analyzed salivary allergens.
METHODS: Locally, Ae. vexans is a major pest, but Ae. aegypti and Cx. quinquefaciatus are not found. We studied 41 subjects living in ...
| Also Ranks for: Mosquito Species | worldwide distribution | ige responses | skin reactions | salivary allergens |
To date, there are no ideal animal models for study of natural sensitization leading to IgE- and lymphocyte-mediated hypersensitivities. We established such a model in which four BALB/c mice were each sensitized by exposure to at least 16 mosquito Aedes aegypti bites, twice a week for 4 weeks. Four non-exposed control mice were also studied. Mosquito A. aegypti head and thorax extract, saliva, and two recombinant salivary allergens (rAed a 1 and rAed a 2) were used in vitro as antigens. ...
| Also Ranks for: Specific Ige | mosquito allergy | sensitized mice | mouse model | subclass responses |
A new recombinant mosquito salivary allergen, rAed a 2: allergenicity, clinical relevance, and cross‐reactivity
[ PUBLICATION ]
BACKGROUND: Mosquito salivary proteins cause allergic reactions in humans. The allergenicity, clinical relevance, and species cross-reactivity of a new 37-kDa recombinant mosquito (Aedes aegypti) salivary allergen, rAed a 2, were evaluated.
METHODS: rAed a 2 was expressed using a baculovirus/insect cell system and purified. Its allergenicity was examined using an enzyme-linked immunosorbent assay (ELISA), ELISA inhibition tests, immunoblots, and skin tests. Epicutaneous tests with the ...
| Also Ranks for: Salivary Allergen | mosquito saliva | skin tests | immunoglobulin insect bites | allergic reactions |
Novel Recombinant Interleukin-13 Peptide-based Vaccine Reduces Airway Allergic Inflammatory Responses in Mice
[ PUBLICATION ]
RATIONALE: Interleukin (IL)-13 plays a pivotal role in the pathogenesis of allergic asthma. Passive administration of its monoclonal antibody or soluble receptor to block overproduced IL-13 has been proven to be effective in controlling airway allergic responses in animal models, but these approaches have disadvantages of short half-lives, high costs, and possible adverse effects.
OBJECTIVES: We sought to develop a novel therapeutic strategy through constructing an IL-13 peptide-based ...
| Also Ranks for: Based Vaccine | inflammatory responses | mice mice | allergic asthma | murine model |
AMP-activated protein kinase (AMPK) is an important cellular energy sensor that is responsible for maintaining systemic and cellular energy balance. Its role in intestinal inflammation remains unclear. Recent studies indicate that AMPK activation initiated by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) participates in modulating inflammatory responses. Inflammatory bowel disease (IBD) has been characterized by sustained intestinal mucosa inflammation, caused mainly by excessive ...
| Also Ranks for: Dextran Sulfate | inflammatory responses | ampk activation | induced acute | intestinal inflammation |
Overexpression of IL-12 and IL-23, which share the p40 subunit, has been implicated in the pathogenesis of Crohn's disease. Targeting these cytokines with monoclonal antibodies has emerged as a new and effective therapy, but one with adverse reactions. In this study, we sought to develop p40 peptide-based virus-like particle vaccines that elicit autoantibodies to IL-12 and IL-23 for a long-term treatment of the disease. Three vaccines (named C, D and F) against the p40 were developed by ...
| Also Ranks for: Murine Colitis | intestinal inflammation | p40 subunit | crohns disease | inbred balb models |
PURPOSE OF REVIEW: Allergic reactions, including severe local and systemic reactions to mosquito bites, are immunological in nature, and involve immunoglobulin E, immunoglobulin G, and T-lymphocyte-mediated hypersensitivities in response to allergens in mosquito saliva. Naturally acquired desensitization to mosquito saliva may occur during childhood or during long-term exposure to mosquitoes. Due to the lack of availability of mosquito salivary preparations for use in skin tests and ...
| Also Ranks for: Mosquito Allergy | allergic reactions | skin tests | salivary proteins | insect bites |
Zhikang Peng: Influence Statistics
| Concept | World rank |
|---|---|
| interleukin33 molecules | #1 |
| vlps inflammatory cell | #1 |
| asthma extension | #1 |
| interleukins mice electron | #1 |
| ova vaccinated mice | #1 |
| naturally acquired sensitization | #1 |
| concurrent antibody responses | #1 |
| ige2 protein | #2 |
| preventative study vaccination | #2 |
| tnbs vaccinated mice | #2 |
| down‐regulates elevated | #2 |
| il17 vaccines | #2 |
| polyclonal ige ige | #2 |
| down‐regulates elevated ige | #2 |
| highly sensitive elispot | #2 |
| il17 peptide | #2 |
| serum il17specific igg | #2 |
| actual salivary antigens | #2 |
| ova therapeutic treatment | #2 |
| unexpectedly vaccinated | #2 |
| tnbs vaccinated | #2 |
| aegypti ige | #2 |
| specific ige pca | #2 |
| species reactive subjects | #2 |
| functional mdsc hsct | #2 |
| employing p40 | #2 |
| typical ige characteristics | #2 |
| antigenicity polyclonal ige | #2 |
| speciesshared allergens | #2 |
| started sensitization | #2 |
| hsct mdsc subsets | #2 |
| ige1 ige2 | #2 |
| redundant il13 effects | #2 |
| local iga airway | #2 |
| doublenegative cell numbers | #2 |
| ige ige1 | #2 |
| cellsecreted molecules interferongamma | #2 |
| mucosal immunization vaccine | #2 |
| elispot immunoglobulin interferon | #2 |
| subjects mosquitos | #2 |
| analyzed salivary allergens | #2 |
| ovalbumindriven th2 patterns | #2 |
| commercial extracts diagnosis | #2 |
| trichosanthin vaccines | #2 |
| charge ige1 | #2 |
| ige2 | #2 |
| vexans bites | #2 |
| trichosanthin specific | #2 |
| immunization il13 | #2 |
| titered long | #2 |
Key People For Mosquito Allergy
Zhikang Peng:Expert Impact
Concepts for whichZhikang Penghas direct influence:Mosquito allergy, Mosquito salivary, Specific ige, Murine colitis, Mosquito bites, Mosquito saliva, Allergic reactions, Chronic colitis.
Zhikang Peng:KOL impact
Concepts related to the work of other authors for whichfor which Zhikang Peng has influence:Intestinal fibrosis, Mosquito bites, Aedes aegypti, Inflammatory bowel disease, Culex quinquefasciatus, Retinoic acid, Salivary proteins.
Tools
Is this your profile? Claim your profile Copy URL Embed Link to your profile |