Prominent publications by Debra A Crumrine

KOL Index score: 14631

Members of the superfamily of nuclear hormone receptors which are obligate heterodimeric partners of the retinoid X receptor may be important in epidermal development. Here, we examined the effects of activators of the receptors for vitamin D3 and retinoids, and of the peroxisome proliferator activated receptors (PPARs) and the farnesoid X-activated receptor (FXR), on the development of the fetal epidermal barrier in vitro. Skin explants from gestational day 17 rats (term is 22 d) are ...

Kjent for Nuclear Hormone |  barrier development |  fetal epidermal |  activators pparalpha |  mature lamellar membranes
KOL Index score: 12695

Previously, we demonstrated that topical applications of peroxisome proliferator-activated receptors (PPARs) and liver X receptor (LXR) activators improve permeability barrier homeostasis. We showed further that stimulation of epidermal differentiation provides one mechanism that could account for such improvement. Here, we studied the effects of these agents on the lipid matrix of the stratum corneum. Hairless mice were treated topically with activators of PPARalpha (WY14643), PPARdelta ...

Kjent for Barrier Homeostasis |  lipid synthesis |  lxr activators |  lamellar body |  hairless mice
KOL Index score: 12243

Prolonged exposure of human epidermis to excess endogenous or exogenous glucocorticoids can result in well-recognized cutaneous abnormalities. Here, we determined whether short-term glucocorticoid treatment would also display adverse effects, specifically on two key epidermal functions, permeability barrier homeostasis and stratum corneum integrity and cohesion, and the basis for such changes. In humans 3 d of treatment with a potent, commonly employed topical glucocorticoid ...

Kjent for Stratum Corneum Integrity |  permeability barrier |  epidermal lipid |  topical glucocorticoid |  hairless microscopy
KOL Index score: 11880

Ceramides in mammalian stratum corneum comprise a heterogeneous mixture of molecular species that subserve the epidermal permeability barrier, an essential function for survival in a terrestrial environment. In addition to a variation of sphingol species, hydroxylation of the amide-linked fatty acids contributes to the diversity of epidermal ceramides. Fatty acid 2-hydroxylase, encoded by the gene FA2H, the mammalian homologue of FAH1 in yeast, catalyzes the synthesis of 2-hydroxy fatty ...

Kjent for Fatty Acid |  keratinocyte differentiation |  fa2h expression |  epidermal ceramides |  2 hydroxylase
KOL Index score: 11647

A barrier to water loss is vital to maintaining life on dry land. Formation of the mammalian skin barrier requires both the essential fatty acid linoleate and the two lipoxygenases 12R-lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3), although their roles are poorly understood. Linoleate occurs in O-linoleoyl-ω-hydroxyceramide, which, after hydrolysis of the linoleate moiety, is covalently attached to protein via the free ω-hydroxyl of the ceramide, forming the corneocyte ...

Kjent for Corneocyte Lipid Envelope |  essential fatty |  skin barrier |  12rlox elox3 |  water loss
KOL Index score: 10152

Nuclear receptors which interact with the retinoid X receptor are involved in the regulation of epidermal differentiation and development. We have recently shown that activators of the peroxisome proliferator-activated receptor and of the farnesoid X-activated receptor accelerate epidermal barrier maturation in fetal rat skin in vitro. In this study we asked whether cutaneous development in utero was affected by peroxisome proliferator-activated receptor or farnesoid X-activated receptor ...

Kjent for Epidermal Differentiation |  barrier development |  receptor activators |  stratum corneum |  nuclear hormone
KOL Index score: 9520

BACKGROUND: Chronologically aged skin exhibits delayed recovery rates after defined barrier insults, with decreased epidermal lipid synthesis, and particularly a reduction in cholesterol synthesis. Prior studies in young mice (< 10 weeks) and humans (20 to 30 years of age) have shown that application of a mixture of cholesterol, ceramides, and essential/nonessential free fatty acids (FFAs) in an equimolar ratio allows normal barrier recovery, whereas any 3:1:1:1 ratio of these four ...

Kjent for Barrier Recovery |  aged skin |  stratum corneum |  young mice |  cholesterol synthesis
KOL Index score: 9239

In this study we investigated whether hyaluronan (HA)-CD44 interaction influences epidermal structure and function. Our data show that CD44 deficiency is accompanied by reduction in HA staining in CD44 knockout (k/o) mouse skin leading to a marked thinning of epidermis versus wild-type mouse skin. A significant delay in the early barrier recovery (following acute barrier disruption) occurs in CD44 k/o versus wild-type mouse skin. To assess the basis for these alterations in CD44 k/o ...

Kjent for Permeability Barrier |  cd44 mouse skin |  hyaluronan receptors |  keratinocyte differentiation |  mouse epidermis
KOL Index score: 9177

We have recently reported that human keratinocytes express both the full-length calcium sensing receptor (CaR) and an alternatively spliced form lacking exon 5, which were suggested to be involved in calcium induced keratinocyte differentiation. To understand further the role of these CaRs, we analyzed the structure of mouse CaRs, and investigated their role using a mouse model in which only the full-length CaR was disrupted. Our results show that both the full-length and the ...

Kjent for Epidermal Differentiation |  calcium sensing receptor |  alternatively spliced |  fulllength car |  mice mice
KOL Index score: 9053

BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory dermatosis now increasingly linked to mutations that alter the structure and function of the stratum corneum. Activators of peroxisome proliferator-activated receptors (PPARs) alpha, beta/delta, and gamma and liver X receptor (LXR) regulate epidermal protein and lipid production, leading to superior barrier function. Additionally, some of these activators exhibit potent antihyperplastic and anti-inflammatory activity in ...

Kjent for Atopic Dermatitis |  peroxisome proliferator |  hairless mice |  ppar lxr |  stratum corneum
KOL Index score: 9022

Mutations in the SPINK5 gene encoding the serine protease (SP) inhibitor, lymphoepithelial-Kazal-type 5 inhibitor (LEKTI), cause Netherton syndrome (NS), a life-threatening disease, owing to proteolysis of the stratum corneum (SC). We assessed here the basis for phenotypic variations in nine patients with "mild", "moderate", and "severe" NS. The magnitude of SP activation correlated with both the barrier defect and clinical severity, and inversely with residual LEKTI expression. LEKTI ...

Kjent for Serine Protease |  netherton syndrome |  lekti expression |  stratum corneum |  spink5 gene
KOL Index score: 8864

Epidermolytic hyperkeratosis is a dominantly inherited ichthyosis, frequently associated with mutations in keratin 1 or 10 that result in disruption of the keratin filament cytoskeleton leading to keratinocyte fragility. In addition to blistering and a severe disorder of cornification, patients typically display an abnormality in permeability barrier function. The nature and pathogenesis of the barrier abnormality in epidermolytic hyperkeratosis are unknown, however. We assessed here, ...

Kjent for Barrier Abnormality |  epidermolytic hyperkeratosis |  cornified envelope |  electron microscopy |  lamellar body
KOL Index score: 8852

Evidence is growing that protease-activated receptor-2 (PAR-2) plays a key role in epithelial inflammation. We hypothesized here that PAR-2 plays a central role in epidermal permeability barrier homeostasis by mediating signaling from serine proteases (SP) in the stratum corneum (SC). Since the SC contains tryptic- and chymotryptic-like activity, we assessed the influence of SP activation/inhibition on barrier function. Acute barrier disruption increases SP activity and blockade by ...

Kjent for Permeability Barrier |  serine protease |  hairless mice |  par2 activation |  stratum corneum
KOL Index score: 8847

Keratinocytes express high levels of 25OHD 1alpha-hydroxylase (1OHase). The product of this enzyme, 1,25-dihydroxyvitamin D (1,25(OH)(2)D), promotes the differentiation of keratinocytes in vitro suggesting an important role for this enzyme in epidermal differentiation. To test whether 1OHase activity is essential for keratinocyte differentiation in vivo we examined the differentiation process in mice null for the expression of the 1alphaOHase gene (1alphaOHase(-/-)). Heterozygotes for ...

Kjent for Permeability Barrier |  25 hydroxyvitamin |  epidermal differentiation |  hydroxylase animals |  epidermis homeostasis
KOL Index score: 8833

The vitamin D receptor (VDR) is a nuclear hormone receptor that controls transcription of target genes. It exerts its biological effects through transcriptional coactivators. Previously, we identified two distinct classes of VDR coactivators, VDR-interacting protein (DRIP) and steroid receptor coactivator (SRC) at different stages of keratinocyte differentiation. Here, we determined the functions of VDR and coactivators in lipid production and permeability barrier formation. Silencing of ...

Kjent for Barrier Formation |  vdr coactivators |  vitamin receptor |  mice mice |  keratinocyte differentiation

Nøkkelpersoner for Stratum Corneum

Toppkols i verden
Peter * *****
stratum corneum barrier function atopic dermatitis
Joke * ********
stratum corneum human skin lipid organization
Howard *** *******
contact dermatitis percutaneous absorption human skin
Richard * * ***
stratum corneum reverse iontophoresis drug delivery
Philip * *****
stratum corneum oral mucosa fatty acids
Donald ****** *******
fatty acids stratum corneum sebum secretion

Debra A Crumrine:Ekspertvirkning

Konsepter somDebra A Crumrinehar direkte innflytelse:Stratum corneum,  Permeability barrier,  Barrier recovery,  Calcium gradient,  Barrier function,  Barrier abnormality,  Permeability barrier homeostasis,  Atopic dermatitis.

Debra A Crumrine:Kol Impact

Konsepter relatert til arbeidet til andre forfattere somfor which Debra A Crumrine har innflytelse:Atopic dermatitis,  Stratum corneum,  Skin barrier,  Wound healing,  Keratinocyte differentiation,  Netherton syndrome,  Hand eczema.



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Department of Dermatology, University of California San Francisco, San Francisco, California. | San Francisco Veterans Affairs Medical Center, Dermatology Service, San Francisco, CA, USA; Department of Dermatology, University of California San Franci