Sergei Nikolaevich Shchelkunov

Sergei Nikolaevich Shchelkunov


Prominent publications by Sergei Nikolaevich Shchelkunov

KOL Index score: 10823

A screening assay for real-time LightCycler (Roche Applied Science, Mannheim, Germany) PCR identification of smallpox virus DNA was developed and compiled in a kit system under good manufacturing practice conditions with standardized reagents. In search of a sequence region unique to smallpox virus, the nucleotide sequence of the 14-kDa fusion protein gene of each of 14 variola virus isolates of the Russian World Health Organization smallpox virus repository was determined and compared ...

Also Ranks for: Smallpox Virus |  viral genes |  time pcr |  strains variola |  simultaneous identification
KOL Index score: 10409

Vaccinia virus complement control protein (VCP) is encoded by vaccinia virus, with its homolog encoded by other pathogenic poxviruses including variola virus. Since rodents are the primary reservoir hosts of cowpox virus (CPV) and since CPV encodes a highly conserved functional homolog of VCP, termed here the inflammation modulatory protein (IMP), the effects of injection of CPV into the footpads of mice was determined in order to study the precise in vivo effects of IMP. Macroscopic ...

Also Ranks for: Cowpox Virus |  cpv imp |  complement control |  modulatory protein |  sequence homology
KOL Index score: 10101

Vaccinia virus complement control protein (VCP) has been shown to possess the ability to inhibit both classical and alternative complement pathway activation. The newly found ability of this protein to bind to heparin has been shown in previous studies to result in uptake by mast cells, possibly promoting tissue persistence. It has also been shown to reduce chemotactic migration of leukocytes by blocking chemokine binding. In addition, this study shows that VCP-through its ability to ...

Also Ranks for: Heparin Binding |  host defense |  molecular interactions |  complement control |  vcp ability
KOL Index score: 10077

Sequencing and computer analysis of the left (52,283 bp) and right (49,649 bp) variable DNA regions of the cowpox virus strain GRI-90 (CPV-GRI) has revealed 51 and 37 potential open reading frames (ORFs), respectively. Comparison of the structure-function organization of these DNA regions of CPV-GRI with those previously published for corresponding regions of genomes of vaccinia virus, strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR); and variola major virus, strains India-1967 ...

Also Ranks for: Cowpox Virus |  sequence analysis |  host range |  amino acid |  viral proteins
KOL Index score: 9647

Tumor necrosis factor (TNF), a potent proinflammatory and antiviral cytokine, is a critical extracellular immune regulator targeted by poxviruses through the activity of virus-encoded family of TNF-binding proteins (CrmB, CrmC, CrmD, and CrmE). The only TNF-binding protein from variola virus (VARV), the causative agent of smallpox, infecting exclusively humans, is CrmB. Here we have aligned the amino acid sequences of CrmB proteins from 10 VARV, 14 cowpox virus (CPXV), and 22 monkeypox ...

Also Ranks for: Cowpox Virus |  binding proteins |  recombinant tnf |  varv crmb |  amino acid
KOL Index score: 8550

Analysis of variola virus nucleotide sequence revealed proteins belonging to several families which provide the virus with the possibility of overcoming the barriers of specific and non-specific host defence against viral infection. The complement-binding proteins, lymphokine-binding proteins, and serine protease inhibitors can be assigned to this type, as can the proteins providing the orthopoxviruses with resistance to interferon. The revealed differences between the genes (proteins) ...

Also Ranks for: Vaccinia Viruses |  variola virus |  sequence homology |  interferon receptors |  viral proteins
KOL Index score: 7865

The data on the structure of the M genome segment of CCHF virus strains from Russia and Central Asia (Tajikistan) are presented. Data obtained have been compared with other available published sequences of the middle segment of strains from China, Nigeria, and Pakistan. It has been found that all the known strains can be divided into four genetic groups, based on the nucleotide sequence of the M genome segment and an amino acid sequence of the glycoprotein precursor it encodes, whereas ...

Also Ranks for: Rna Segment |  genetic characterization |  virus strains |  congo hemorrhagic |  genome viral
KOL Index score: 7842

Sequencing and computer analysis of a variola major virus strain India-1967 (VAR-IND) genome segment (53,018 bp) from the right terminal region has been carried out. Fifty-nine potential open reading frames (ORFs) of over 60 amino acid residues were identified. Structure-function organization of the VAR-IND DNA segment was compared with the previously reported sequences from the analogous genomic regions of vaccinia virus strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR) and ...

Also Ranks for: Viral Genome |  nucleotide sequence |  virus strain |  variola major |  amino acid
KOL Index score: 7624

Although polyomavirus JC (JCV) is the proven pathogen of progressive multifocal leukoencephalopathy, the fatal demyelinating disease, this virus is ubiquitous as a usually harmless symbiote among human beings. JCV propagates in the adult kidney and excretes its progeny in urine, from which JCV DNA can readily be recovered. The main mode of transmission of JCV is from parents to children through long cohabitation. In this study, we collected a substantial number of urine samples from ...

Also Ranks for: Virus Dna |  viral emigration |  africa asia |  subtypes jcv |  immigration genetics
KOL Index score: 7523

The nucleotide sequence of the 3913 base pair XhoI O fragment located in an evolutionary variable region adjacent to the right end of the genome of ectromelia virus (EMV) was determined. The sequence contains two long open reading frames coding for putative proteins of 559 amino acid residues (p65) and 344 amino acid residues (p39). Amino acid database searches showed that p39 is closely related to vaccinia virus (VV), strain WR, B22R gene product (C12L gene product of strain ...

Also Ranks for: Vaccinia Virus |  amino acid |  putative proteins |  sequence dna |  serine protease inhibitors
KOL Index score: 7476

Summary.Cowpox virus (CPXV) strain GRI-90 contains six genes encoding kelch-like proteins. All six proteins contain both, the N-terminal BTB domain and the C-terminal kelch domain. We constructed mutant variants of a CPXV strain with targeted deletions of one to four genes of the kelch family, namely D11L, C18L, G3L, and A57R. As kelch genes are located in terminal variable regions of the CPXV genome, we studied the relationship of these genes with integral biological characteristics ...

Also Ranks for: Cowpox Virus |  viral humans |  kelch genes |  mice mice |  cpxv strain
KOL Index score: 6945

DNA of the variola major virus strain India-1967 in the region of HindIII M, L, I fragments has been sequenced. Analysis of this sequence of 18029 bp revealed 19 potential open reading frames (ORFs). Four proposed proteins (L2R, H9R, L5L, L6R) contain metal-binding domains. Comparison of the variola virus (VAR) and vaccinia virus strain Copenhagen (COP) sequences show that the main differences are between proteins L1R and I5R. L1R contains 6 additional amino acid residues on the ...

Also Ranks for: Variola Virus |  nucleotide sequence |  viral genome |  amino acid |  analysis dna
KOL Index score: 6832

The nucleotide sequences of genome DNAs and the deduced amino acid sequences of proteins from potential open reading frames (ORFs) of variola smallpox viruses from outbreaks in India in 1967 and in Bangladesh in 1975 have been compared and the analyses of the sequences are updated. Alignment of the DNAs revealed 99.3% base sequence identity. Of the 200 potential encoded proteins of each virus, 122 were identical, 42 showed substitution of a single amino acid, 11 showed two residues ...

Also Ranks for: Viral Genome |  variola virus |  dna sequences |  sequence homology |  reading frames
KOL Index score: 6717

Computer analysis of full coding sequences of variola major virus strain India-1967 genome and vaccinia virus strain Copenhagen genome have been carried out. A wide set of proteins containing ankyrin-like repeats have been identified for both viruses. Only three proteins of this family of the studied viruses are highly homologous. The rest of the proteins are different. The possible role of such proteins in determination of virus tissue tropism is discussed.

Also Ranks for: Vaccinia Virus |  viral proteins |  coding sequences |  copenhagen genome |  ankyrinlike repeats
KOL Index score: 6686

Alastrim variola minor virus, which causes mild smallpox, was first recognized in Florida and South America in the late 19th century. Genome linear double-stranded DNA sequences (186,986 bp) of the alastrim virus Garcia-1966, a laboratory reference strain from an outbreak associated with 0.8% case fatalities in Brazil in 1966, were determined except for a 530-bp fragment of hairpin-loop sequences at each terminus. The DNA sequences (EMBL Accession No. Y16780) showed 206 potential open ...

Also Ranks for: Virus Genome |  viral proteins |  variola minor |  amino acid |  reading frames

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Sergei Nikolaevich Shchelkunov:Expert Impact

Concepts for whichSergei Nikolaevich Shchelkunovhas direct influence:Variola virus,  Cowpox virus,  Vaccinia virus,  Viral genome,  Nucleotide sequence,  Viral genes,  Smallpox virus,  Amino acid.

Sergei Nikolaevich Shchelkunov:KOL impact

Concepts related to the work of other authors for whichfor which Sergei Nikolaevich Shchelkunov has influence:Vaccinia virus,  Viral proteins,  Smallpox vaccine,  Human monkeypox,  Amino acid,  Complement activation,  Infected cells.



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Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Street, Novosibirsk 630090, Russia;, | State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia. | State Research