• Disease
  • Yersinia
  • Yersinia Pestis
  • Richard W Titball

    Prominent publications by Richard W Titball

    KOL Index score: 16453

    Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and F. tularensis subsp. novicida. We developed a DNA microarray based on 1,832 clones from a shotgun library used for sequencing of the highly ...

    Also Ranks for: Tularensis Subsp |  microarray analysis |  highly virulent |  genome bacterial |  mediasiatica novicida
    KOL Index score: 13120

    We have determined the sequence of the gene cluster encoding the O antigen in Francisella novicida and compared it to the previously reported O-antigen cluster in Francisella tularensis subsp. tularensis. Immunization with purified lipopolysaccharide (LPS) from F. tularensis subsp. tularensis or F. novicida protected against challenge with Francisella tularensis subsp. holarctica and F. novicida, respectively. The LPS from F. tularensis subsp. tularensis did not confer protection against ...

    Also Ranks for: Tularensis Subsp |  francisella novicida |  mice mice |  virulence determinants |  intracellular survival
    KOL Index score: 13060

    The caf operon from Yersinia pestis encoding the structural subunit (caf1), the molecular chaperone (caf1M), the outer membrane anchor (caf1A), and the regulatory protein (caf1R) was cloned into Salmonella typhimurium SL3261 aroA. The recombinant Salmonella organisms were encapsulated when cultured at 37 degrees C but not when cultured at 28 degrees C. Oral inoculation of mice with the recombinant Salmonella induced predominantly an immunoglobulin G2a response to F1 antigen, and isolated ...

    Also Ranks for: F1 Antigen |  yersinia pestis |  salmonella typhimurium |  aroa mutant |  mice recombinant
    KOL Index score: 12050

    Yersinia pestis, the causative agent of plague, diverged from Yersinia pseudotuberculosis, an enteric pathogen, an estimated 1500-20,000 years ago. Genetic characterization of these closely related organisms represents a useful model to study the rapid emergence of bacterial pathogens that threaten mankind. To this end, we undertook genome-wide DNA microarray analysis of 22 strains of Y. pestis and 10 strains of Y. pseudotuberculosis of diverse origin. Eleven Y. pestis DNA loci were ...

    Also Ranks for: Yersinia Pseudotuberculosis |  dna microarrays |  strains pestis |  bacterial evolution |  causative agent
    KOL Index score: 12025

    Bubonic and pneumonic plague are caused by the bacterium Yersinia pestis. The V antigen of Y. pestis is a protective antigen against plague. In this study, an aroA attenuated strain of Salmonella enterica serovar Typhimurium (SL3261) has been used to deliver the Y. pestis V antigen as a candidate oral plague vaccine. SL3261 was transformed with the expression plasmid pTrc-LcrV, containing the lcrV gene encoding V antigen. Immunoblot analysis showed V antigen expression in SL3261 in vitro ...

    Also Ranks for: Salmonella Enterica |  yersinia pestis |  bacterial antigens |  oral animals antibodies |  mice plague
    KOL Index score: 11909

    Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infectious disease for which there is no vaccine. B. pseudomallei is listed as a tier 1 select agent, and as current therapeutic options are limited due to its natural resistance to most antibiotics, the development of new antimicrobial therapies is imperative. To identify drug targets and better understand the complex B. pseudomallei genome, we sought a genome-wide approach to identify lethal gene targets. ...

    Also Ranks for: Essential Genes |  burkholderia pseudomallei |  antimicrobial development |  insertional sequence analysis |  genome bacterial
    KOL Index score: 11594

    The Gram-negative bacterium Yersinia pestis is the causative agent of the systemic invasive infectious disease classically referred to as plague1, and has been responsible for three human pandemics: the Justinian plague (sixth to eighth centuries), the Black Death (fourteenth to nineteenth centuries) and modern plague (nineteenth century to the present day). The recent identification of strains resistant to multiple drugs2 and the potential use of Y. pestis as an agent of biological ...

    Also Ranks for: Causative Agent |  yersinia pestis |  genome sequence |  bacterial humans |  gene transfer
    KOL Index score: 11573

    BACKGROUND: Burkholderia pseudomallei is the causative agent of melioidosis, a tropical disease of humans with a variable and often fatal outcome. In murine models of infection, different strains exhibit varying degrees of virulence. In contrast, two related species, B. thailandensis and B. oklahomensis, are highly attenuated in mice. Our aim was to determine whether virulence in mice is reflected in macrophage or wax moth larvae (Galleria mellonella) infection models.

    RESULTS: B. ...

    Also Ranks for: Galleria Mellonella |  infection models |  burkholderia pseudomallei |  macrophages mice |  causative agent
    KOL Index score: 11236

    V antigen of Yersinia pestis is a multifunctional protein that has been implicated as a protective antigen, a virulence factor, and a regulatory protein. A series of V-antigen truncates expressed as glutathione S-transferase (GST) fusion proteins (GST-V truncates) have been cloned and purified to support immunogenicity and functionality studies of V antigen. Immunization studies with GST-V truncates have identified two regions of V antigen that confer protection against Y. pestis 9B (a ...

    Also Ranks for: Yersinia Pestis |  monoclonal antigens |  amino acids |  protection plague |  protective antigen
    KOL Index score: 11099

    The lack of novel antibiotics for more than a decade has placed increased pressure on existing therapies to combat the emergence of multidrug-resistant (MDR) bacterial pathogens. This study evaluated the Galleria mellonella insect model in determining the efficacy of available antibiotics against planktonic and biofilm infections of MDR Pseudomonas aeruginosa and Klebsiella pneumoniae strains in comparison with in vitro minimum inhibitory concentration (MIC) determination. In general, in ...

    Also Ranks for: Antibiotic Efficacy |  galleria mellonella |  pseudomonas aeruginosa |  klebsiella pneumoniae |  animal drug resistance
    KOL Index score: 11032

    A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of Francisella tularensis subsp. tularensis Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria. Ten pairs of overlapping primers were designed to amplify the O antigen biosynthetic cluster by PCR from nine strains of F. tularensis. Although the gene ...

    Also Ranks for: Tularensis Subsp |  schu s4 |  structural analysis |  gene cluster |  molecular dna
    KOL Index score: 10890

    Bacillus anthracis is the causative agent of anthrax, a disease that affects wildlife, livestock, and humans. Protection against anthrax is primarily afforded by immunity to the B. anthracis protective antigen (PA), particularly PA domains 4 and 1. To further the development of an orally delivered human vaccine for mass vaccination against anthrax, we produced Salmonella enterica serovar Typhimurium expressing full-length PA, PA domains 1 and 4, or PA domain 4 using codon-optimized PA ...

    Also Ranks for: Salmonella Enterica |  oral administration |  protective antigen |  based vaccine |  bacillus anthracis
    KOL Index score: 10716

    In this study, the protection afforded against aerosolized Yersinia pestis by injection of an alhydrogel-adsorbed sub-unit vaccine has been compared with that given by an existing killed whole cell vaccine licensed for human use. The sub-unit vaccine protected mice against exposure to > 10(4) colony-forming units (c.f.u.) of virulent plague organisms (100 LD50 doses), whereas the whole cell vaccine provided only 50% protection against 1.8 x 10(3) c.f.u. In sub-unit vaccinees, IgG to each ...

    Also Ranks for: Unit Vaccine |  pneumonic plague |  bacterial antigens |  immunized animals |  spleen cells
    KOL Index score: 10529

    Melioidosis is a severe infectious disease of animals and humans caused by the Gram-negative intracellular pathogen Burkholderia pseudomallei. An Inv/Mxi-Spa-like type III protein secretion apparatus, encoded by the B. pseudomallei bsa locus, facilitates bacterial invasion of epithelial cells, escape from endocytic vesicles and intracellular survival. This study investigated the role of the Bsa type III secretion system in the pathogenesis of melioidosis in murine models. B. pseudomallei ...

    Also Ranks for: Type Iii |  murine models |  protective efficacy |  balb mice |  burkholderia pseudomallei
    KOL Index score: 10496

    To investigate Yersinia pathogenicity and the evolutionary divergence of the genus, the effect of pathogenic yersiniae on the model organism Caenorhabditis elegans was studied. Three strains of Yersinia pestis, including a strain lacking pMT1, caused blockage and death of C. elegans; one strain, lacking the haemin storage (hms) locus, caused no effect. Similarly, 15 strains of Yersinia enterocolitica caused no effect. Strains of Yersinia pseudotuberculosis showed different levels of ...

    Also Ranks for: Biofilm Formation |  yersinia pseudotuberculosis |  biotic surface |  caenorhabditis elegans |  infections animals


    Richard W Titball: Influence Statistics

    Sample of concepts for which Richard W Titball is among the top experts in the world.
    Concept World rank
    elegans 37 degrees #1
    biodefence #1
    subspecies holarctica hn63 #1
    yersinia pestis feeding #1
    copurified lipopolysaccharide #1
    responses lvs vaccine #1
    tocpamrna levels #1
    multidomain fold #1
    gastrointestinal diseases piglets #1
    diagnosis pneumonic tularemia #1
    betatoxin clostridium perfringens #1
    melioidosis vaccine #1
    cellular sensitivity cpplc #1
    virulence mips #1
    dangerous pathogens category #1
    systems tularensis #1
    crystal structure flgkbp #1
    hexasaccharide glycoconjugate #1
    attenuated vaccines plague #1
    oantigen biosynthetic operon #1
    pila pilin #1
    necrotic enteritis▿ #1
    protein proposed role #1
    carriers heterologous antigens #1
    tularensis intracellular survival #1
    cpplc cellular sensitivity #1
    unconjugated f1antigen pbs #1
    subsequent challenge pseudomallei #1
    βtoxin chapter #1
    antibiotics burkholderia #1
    models pseudomallei #1
    mallei antimicrobials #1
    bpss1823 #1
    expression plc2 gene #1
    mice adjuvanted pa #1
    alphatoxin fold #1
    cer89l43 #1
    secondary solubility toxins #1
    differences αtoxin production #1
    tularensis protection #1
    mutant francisella novicida #1
    strain tularensis subsp #1
    elegans pa14 #1
    virulent pestis protection #1
    production major toxins #1
    sequence homology alphatoxin #1
    hica toxin #1
    pcr f1 fusions #1
    antimicrobial development #1
    k41h #1

    Key People For Yersinia Pestis

    Top KOLs in the world
    Robert D Perry
    yersinia pestis biofilm formation bacterial genes
    Robert R Brubaker
    yersinia pestis 37 degrees bacterial genes
    Jacqueline D Fetherston
    yersinia pestis bacterial genes biofilm formation
    Elisabeth Carniel
    yersinia pestis pathogenicity island bubonic plague
    Richard W Titball
    yersinia pestis clostridium perfringens francisella tularensis
    Susan C Straley
    yersinia pestis yop secretion 37 degrees

    Richard W Titball:Expert Impact

    Concepts for whichRichard W Titballhas direct influence:Yersinia pestis,  Clostridium perfringens,  Francisella tularensis,  Burkholderia pseudomallei,  Tularensis subsp,  Galleria mellonella,  Bacterial antigens,  Yersinia pseudotuberculosis.

    Richard W Titball:KOL impact

    Concepts related to the work of other authors for whichfor which Richard W Titball has influence:Francisella tularensis,  Yersinia pestis,  Clostridium perfringens,  Burkholderia pseudomallei,  Necrotic enteritis,  Bacillus anthracis,  Escherichia coli.



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    College of Life and Environmental Sciences - Biosciences, University of Exeter, Exeter, Devon, EX4 4QD, UK | Department of Biosciences, College of Life and Environmental Sciences—Biosciences, University of Exeter, Exeter EX4 4QD, UK;, C.Mueller@exete