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The draft genome of the carcinogenic human liver fluke Clonorchis sinensis: Influence Statistics

Expert Impact

Concepts for which they have has direct influence: Liver fluke , Draft genome , Clonorchis sinensis , Fluke clonorchis , Human liver , Fatty acid , Key genes .

Key People For Liver Fluke

Top KOLs in the world
#1
Paul James Brindley
schistosoma mansoni opisthorchis viverrini liver fluke
#2
Banchob Sripa
opisthorchis viverrini liver fluke helicobacter pylori
#3
Paiboon Sithithaworn
opisthorchis viverrini lao pdr northeast thailand
#4
Ian Fairweather
fasciola hepatica liver fluke northern ireland
#5
Thewarach Laha
opisthorchis viverrini liver fluke lao pdr
#6
Alex C Loukas
hookworm infection opisthorchis viverrini schistosoma mansoni

The draft genome of the carcinogenic human liver fluke Clonorchis sinensis

Abstract

. BACKGROUND: Clonorchis sinensis is a carcinogenic human liver fluke that is widespread in Asian countries. Increasing infection rates of this neglected tropical disease are leading to negative economic and public health consequences in affected regions. Experimental and epidemiological studies have shown a strong association between the incidence of cholangiocarcinoma and the infection rate of C. sinensis. To aid research into this organism, we have sequenced its genome. RESULTS: We combined de novo sequencing with computational techniques to provide new information about the biology of this liver fluke. The assembled genome has a total size of 516 Mb with a scaffold N50 length of 42 kb. Approximately 16,000 reliable protein-coding gene models were predicted. Genes for the complete pathways for glycolysis, the Krebs cycle and fatty acid metabolism were found, but key genes involved in fatty acid biosynthesis are missing from the genome, reflecting the parasitic lifestyle of a liver fluke that receives lipids from the bile of its host. We also identified pathogenic molecules that may contribute to liver fluke-induced hepatobiliary diseases. Large proteins such as multifunctional secreted proteases and tegumental proteins were identified as potential targets for the development of drugs and vaccines. CONCLUSIONS: This study provides valuable genomic information about the human liver fluke C. sinensis and adds to our knowledge on the biology of the parasite. The draft genome will serve as a platform to develop new strategies for parasite control.